E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3) [PDF]
Microbial Cell Factories, 2018 Background Escherichia coli is one of the most widely used hosts for recombinant protein production in academia and industry. Strain BL21(DE3) is frequently employed due to its advantageous feature of lacking proteases which avoids degradation of target ...
Johanna Hausjell +4 more
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A comparative study on the genomes, transcriptomes, and metabolic properties of Escherichia coli strains Nissle 1917, BL21(DE3), and MG1655 [PDF]
Engineering Microbiology, 2022 Escherichia coli is the most well-studied model prokaryote and has become an indispensable host for the biotechnological production of proteins and biochemicals. In particular, the probiotic status of one E. coli strain, E.
Linlin Zhao +5 more
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PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP [PDF]
Journal of the Indonesian Tropical Animal Agriculture, 2014 This research was aimed at producing a crude intracellular phytase characterized from recombinantbacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+)+pEAS1 into competent E. coli BL21 and E.
A.M.P. Nuhriawangsa +3 more
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The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica [PDF]
Pathogens, 2021 Biological nematicides have been widely used to lower the losses generated by phytoparasitic nematodes. The purpose of this study was to evaluate the nematicidal effects of Escherichia coli BL21(DE3) against Meloidogyne javanica and to identify ...
Yanfei Xia +7 more
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Cascaded processing enables continuous upstream processing with E. coli BL21(DE3). [PDF]
Sci Rep, 2021 AbstractIn many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated.
Kittler S +8 more
europepmc +5 more sources
Efficient anaerobic consumption of D-xylose by E. coli BL21(DE3) via xylR adaptive mutation [PDF]
BMC Microbiology, 2021 Background Microorganisms can prioritize the uptake of different sugars depending on their metabolic needs and preferences. When both D-glucose and D-xylose are present in growth media, E.
Jung Min Heo, Hyun Ju Kim, Sang Jun Lee
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Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production [PDF]
Microbial Cell Factories, 2021 Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (PlacUV5 ...
Fei Du +6 more
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Reduced Background Expression and Improved Plasmid Stability with pET Vectors in BL21 (DE3) [PDF]
BioTechniques, 2000 The T7 polymerase-based pET System™ is one of the most powerful and widely used prokaryotic expression systems available today. Expression of even slightly toxic gene products in BL21 (DE3), however, has been problematic due to basal expression, which ...
Shi-hao Pan, Bruce A. Malcolm
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A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3). [PDF]
Biotechnol Biofuels, 2018 Styrene is a versatile commodity petrochemical used as a monomer building-block for the synthesis of many useful polymers. Although achievements have been made on styrene biosynthesis in microorganisms, several bottleneck problems limit factors for further improvement in styrene production.A two-step styrene biosynthesis pathway was developed and ...
Liu C +11 more
europepmc +5 more sources
Genomic and transcriptomic landscape of Escherichia coli BL21(DE3). [PDF]
Nucleic Acids Res, 2017 Escherichia coli BL21(DE3) has long served as a model organism for scientific research, as well as a workhorse for biotechnology. Here we present the most current genome annotation of E. coli BL21(DE3) based on the transcriptome structure of the strain that was determined for the first time.
Kim S +5 more
europepmc +5 more sources