Results 11 to 20 of about 143,695 (288)

Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3). [PDF]

PLoS ONE, 2011
The Escherichia coli B strain BL21(DE3) has had a profound impact on biotechnology through its use in the production of recombinant proteins. Little is understood, however, regarding the physiology of this important E. coli strain. We show here that BL21(
Constanze Pinske, Markus Bönn, Sara Krüger, Ute Lindenstrauss, R Gary Sawers   +4 more
doaj   +6 more sources

بهینه‏سازی کدونی و مقایسه‏ی بیان فاکتور رشد عصبی بتا انسانی (β-NGF) بصورت نوترکیب در میزبان‏های باکتریایی( BL21(DE3 و BL21(DE3)pLysS [PDF]

زیست‌شناسی کاربردی, 2017
فاکتور رشد عصبی بتا (β-NGF) برای اولین بار بدلیل نقش حیاتی‌اش در رشد و بقای سلول‏های عصبی مورد مطالعه قرار گرفت. این پروتئین که به خانواده‌ی نوروتروفین‌ها تعلق دارد در درمان بیماری‌های تخریب عصبی نظیر آلزایمر دارای نقش قابل ملاحظه‏ای می‏باشد. بدین منظور،
معصومه رجبی بذل, زهرا حاجی حسن, مونا سهرابی   +2 more
doaj   +2 more sources

Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3). [PDF]

PLoS ONE, 2018
Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins.
Hanseol Kim, Sinyeon Kim, Sung Ho Yoon
doaj   +2 more sources

A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3). [PDF]

Microb Cell Fact, 2020
Abstract Escherichia coli BL21 is arguably the most popular host for industrial production of proteins, and industrial fermentations are often plagued by phage infections. The CRISPR/Cas system is guided by a gRNA to cleave a specific DNA cassette, which can be developed into a highly efficient programable phage defense system.
Liu L, Zhao D, Ye L, Zhan T, Xiong B, Hu M, Bi C, Zhang X.   +7 more
europepmc   +5 more sources

Tailoring Escherichia coli BL21 (DE3) for preferential xylose utilization via metabolic and regulatory engineering. [PDF]

Appl Microbiol Biotechnol
Abstract Xylose is the most abundant pentose in nature. However, it is usually obtained in mixtures with glucose, leading to carbon catabolite repression in many microorganisms. Among E. coli lineages, significant metabolic and regulatory differences exist, requiring distinct metabolic engineering strategies to develop a xylose-
Molina-Vázquez ER, Caspeta L, Gosset G, Martínez A.   +3 more
europepmc   +3 more sources

Characterisation of the bacterial biosensor GMG in E-coli BL21 (DE3)

Engineering Biology, 2018
In this work, a gold biosensor has been constructed and characterised in Escherichia coli (E. coli). Though gold biosensors have been previously reported, a NOT gate gold biosensing genetic circuit was constructed using an orthogonal inverter system as ...
Rashmi Rajasabhai, Maybelle Kho Go, Yew Wen Shan   +2 more
doaj   +2 more sources

Cloning and Expression of Recombinant Human Interleukin 1 Receptor Antagonist in Escherichia Coli Strains, BL21 (DE3), Rosetta (DE3), and Origami (DE3)

مجله دانشکده پزشکی اصفهان, 2019
Background: Recombinant human interleukin-1 receptor antagonist (IL-1RA) is a protein with 153 amino acids and molecular weight of 16.83 kDa. This drug protein is known as Anakinra, and has an effective application in the treatment of rheumatoid ...
Hanieh Fardgoli, Seyed Nezameddin Hoseini, Setareh Haghighat   +2 more
doaj   +3 more sources

Production of Recombinant Polyepitopic HPV16-E6 Antigen: the Effects of various Strains and Expression Conditions [PDF]

Biological Journal of Microorganism, 2022
Introduction: Cervical cancer is the leading cause of morbidity and cancer mortality in women throughout the world and persistent infection with human papillomavirus (HPV) is the main etiology for the development of this cancer.
Bahareh Bahmani, Zahra Aminibayat, Mohammad Mehdi Ranjbar, Nahid Bakhtiari, Amir‑Hassan Zarnani   +4 more
doaj   +1 more source

Interleukin gene cloning and expression in E. coli

Annals of Medicine, 2021
Introduction Cytokines are a large category of protins; they play various functions from inflammation response to cancer development and sepsis. Over-expression or problems in production control of this small peptides can lead to the development of ...
Filipa Oliveira, Fernando Cardoso
doaj   +1 more source

Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3) [PDF]

, 2017
Membrane protein production is usually toxic to E. coli. However, using genetic screens strains can be isolated in which the toxicity of membrane protein production is reduced, thereby improving production yields. Best known examples are the C41(DE3) and
Baumgarten, Thomas, Bonde, Ida, de Gier, Jan-Willem, Eriksson, Jonas, Heipieper, Hermann J., Herrgard, Markus, Löw, Mirjam, Nørholm, Morten, Schlegel, Susan, Slotboom, Dirk Jan, Wagner, Samuel   +10 more
core   +9 more sources