Results 11 to 20 of about 101,082 (154)
Characterisation of the bacterial biosensor GMG in E-coli BL21 (DE3)
Engineering Biology, 2018 In this work, a gold biosensor has been constructed and characterised in Escherichia coli (E. coli). Though gold biosensors have been previously reported, a NOT gate gold biosensing genetic circuit was constructed using an orthogonal inverter system as ...
Rashmi Rajasabhai +2 more
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مجله دانشکده پزشکی اصفهان, 2019 Background: Recombinant human interleukin-1 receptor antagonist (IL-1RA) is a protein with 153 amino acids and molecular weight of 16.83 kDa. This drug protein is known as Anakinra, and has an effective application in the treatment of rheumatoid ...
Hanieh Fardgoli +2 more
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Production of Recombinant Polyepitopic HPV16-E6 Antigen: the Effects of various Strains and Expression Conditions [PDF]
Biological Journal of Microorganism, 2022 Introduction: Cervical cancer is the leading cause of morbidity and cancer mortality in women throughout the world and persistent infection with human papillomavirus (HPV) is the main etiology for the development of this cancer.
Bahareh Bahmani +4 more
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Interleukin gene cloning and expression in E. coli
Annals of Medicine, 2021 Introduction Cytokines are a large category of protins; they play various functions from inflammation response to cancer development and sepsis. Over-expression or problems in production control of this small peptides can lead to the development of ...
Filipa Oliveira, Fernando Cardoso
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Biotechnology for Biofuels, 2020 Background Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source.
Tong Wu +8 more
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A Dual-Bacterial Coupled Fermentation Strategy for Nicotinamide Mononucleotide Synthesis [PDF]
Shipin Kexue, 2023 In this study, a dual-bacterial coupled fermentation system containing nicotinamide nucleoside kinase (NRK) and polyphosphatase (PPK) was constructed, and the application of PPK-based ATP regeneration system in NMN production was achieved.
SUN Ting, ZHANG Hongtao, YANG Feng, CHAI Wengang, XUE Haoyang, TAN Shuyin
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BMC Research Notes, 2018 Objective The genome of Vibrio cholerae has three paralog genes encoding for distinct pyruvate kinases. We were interested in elucidating whether they were expressed, and contributed to the pyruvate kinase activity of V. cholerae.
Zoe Alba-Martínez +2 more
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Efficient Expression and Enzymatic Properties of L-Threonine Dehydrogenase from Escherichia coli [PDF]
Shipin Kexue, 2023 Our aim was to improve the catalytic efficiency of L-threonine dehydrogenase (L-TDH) on L-threonine dehydrogenation to synthesize ethyl L-2-aminoacetate. An L-TDH gene from Escherichia coli was mined and solubly expressed in E.
LIU Xinxin, WANG Yao, SHI Hongling, YAO Lunguang, WANG Xian, TANG Cunduo
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Engineered Escherichia coli strains as platforms for biological production of isoprene
FEBS Open Bio, 2020 Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21 ...
Hyeok‐Won Lee +7 more
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Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3). [PDF]
PLoS ONE, 2011 The Escherichia coli B strain BL21(DE3) has had a profound impact on biotechnology through its use in the production of recombinant proteins. Little is understood, however, regarding the physiology of this important E. coli strain. We show here that BL21(
Constanze Pinske +4 more
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