Results 21 to 30 of about 143,695 (288)

Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies

open access: yesBiotechnology for Biofuels, 2020
Background Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source.
Tong Wu   +8 more
doaj   +1 more source

A Dual-Bacterial Coupled Fermentation Strategy for Nicotinamide Mononucleotide Synthesis [PDF]

open access: yesShipin Kexue, 2023
In this study, a dual-bacterial coupled fermentation system containing nicotinamide nucleoside kinase (NRK) and polyphosphatase (PPK) was constructed, and the application of PPK-based ATP regeneration system in NMN production was achieved.
SUN Ting, ZHANG Hongtao, YANG Feng, CHAI Wengang, XUE Haoyang, TAN Shuyin
doaj   +1 more source

Efficient Expression and Enzymatic Properties of L-Threonine Dehydrogenase from Escherichia coli [PDF]

open access: yesShipin Kexue, 2023
Our aim was to improve the catalytic efficiency of L-threonine dehydrogenase (L-TDH) on L-threonine dehydrogenation to synthesize ethyl L-2-aminoacetate. An L-TDH gene from Escherichia coli was mined and solubly expressed in E.
LIU Xinxin, WANG Yao, SHI Hongling, YAO Lunguang, WANG Xian, TANG Cunduo
doaj   +1 more source

Exploring the differences between the three pyruvate kinase isozymes from Vibrio cholerae in a heterologous expression system

open access: yesBMC Research Notes, 2018
Objective The genome of Vibrio cholerae has three paralog genes encoding for distinct pyruvate kinases. We were interested in elucidating whether they were expressed, and contributed to the pyruvate kinase activity of V. cholerae.
Zoe Alba-Martínez   +2 more
doaj   +1 more source

Engineered Escherichia coli strains as platforms for biological production of isoprene

open access: yesFEBS Open Bio, 2020
Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21 ...
Hyeok‐Won Lee   +7 more
doaj   +1 more source

Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3) [PDF]

open access: yesGenome Announcements, 2017
ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21 ...
Goffin, Philippe, Dehottay, Philippe
openaire   +2 more sources

Microbial Cell Factories / Evaluation of three industrial Escherichia coli strains in fed-batch cultivations during high-level SOD protein production [PDF]

open access: yes, 2013
Background: In the biopharmaceutical industry, Escherichia coli (E. coli) strains are among the most frequently used bacterial hosts for producing recombinant proteins because they allow a simple process set-up and they are Food and Drug Administration ...
Bayer, Karl   +4 more
core   +2 more sources

Site‐directed mutagenesis improves the practical application of L‐glutamic acid decarboxylase in Escherichia coli

open access: yesEngineering in Life Sciences, 2023
γ‐Aminobutyric acid (GABA) is a kind of non‐proteinogenic amino acid which is highly soluble in water and widely used in the food and pharmaceutical industries.
Liu Fengmin   +5 more
doaj   +1 more source

Enhanced expression of recombinant beta toxin of Clostridium perfringens type B using a commercially available Escherichia coli strain

open access: yesOnderstepoort Journal of Veterinary Research, 2016
Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C.
Fatemah Bakhshi   +2 more
doaj   +1 more source

Modification and Expression of Beta-1,4-Endoglucanase encoding sequences of fungal origin in Escherichia coli BL21.

open access: yesRevista Colombiana de Biotecnología, 2020
Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle   +2 more
doaj   +1 more source

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