Results 21 to 30 of about 101,082 (154)
Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C.
Fatemah Bakhshi +2 more
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γ‐Aminobutyric acid (GABA) is a kind of non‐proteinogenic amino acid which is highly soluble in water and widely used in the food and pharmaceutical industries.
Liu Fengmin +5 more
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Cloning and expression of native streptokinase in E. coli BL21(DE3)
Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria. Streptokinase is one of the most important thrombolytic agents that was frequently employed treatment of myocardial infarction. In this study, we amplified the SK gene isolated from Streptococcus pyogenes. The sequence of SK was recorded in
Almuthana K. Hameed +2 more
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Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle +2 more
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Transformation Protocol for BL21(DE3) Competent Cells (C2527I) v2 [PDF]
This transformation protocol is for the C2527I cells. (For the C2527H protocol, see here.)
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The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the ...
Rafida Razali +2 more
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Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3) [PDF]
ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21 ...
Goffin, Philippe, Dehottay, Philippe
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PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP
This research was aimed at producing a crude intracellular phytase characterized from recombinantbacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+)+pEAS1 into competent E. coli BL21 and E.
A.M.P. Nuhriawangsa +3 more
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High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. [PDF]
Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.
Yao Nie +6 more
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Effect of Desaturase Gene Overexpression on Fatty Acid Synthesis in Escherichia coli [PDF]
In order to obtain efficient engineered strains for oil production, the desaturase gene from Bacillus subtilis HB1310, an endophytic bacterium isolated from walnut, was expressed in Escherichia coli BL21 (DE3), the single-gene-expression strains BL21(DE3)
YE Jing, XU Siyuan, ZHANG Qin, QIAN Cheng, CAO Juanjuan, ZHAO Pei
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