Results 21 to 30 of about 101,082 (154)

Enhanced expression of recombinant beta toxin of Clostridium perfringens type B using a commercially available Escherichia coli strain

open access: yesOnderstepoort Journal of Veterinary Research, 2016
Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C.
Fatemah Bakhshi   +2 more
doaj   +1 more source

Site‐directed mutagenesis improves the practical application of L‐glutamic acid decarboxylase in Escherichia coli

open access: yesEngineering in Life Sciences, 2023
γ‐Aminobutyric acid (GABA) is a kind of non‐proteinogenic amino acid which is highly soluble in water and widely used in the food and pharmaceutical industries.
Liu Fengmin   +5 more
doaj   +1 more source

Cloning and expression of native streptokinase in E. coli BL21(DE3)

open access: yesInternational journal of health sciences, 2022
Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria. Streptokinase is one of the most important thrombolytic agents that was frequently employed treatment of myocardial infarction. In this study, we amplified the SK gene isolated from Streptococcus pyogenes. The sequence of SK was recorded in
Almuthana K. Hameed   +2 more
openaire   +1 more source

Modification and Expression of Beta-1,4-Endoglucanase encoding sequences of fungal origin in Escherichia coli BL21.

open access: yesRevista Colombiana de Biotecnología, 2020
Lignocellulose is the main and most abundant component of biomass. Annually, 200 million tons are generated in the world. Colombia has a high production of lignocellulosic residues that can be used in many industrial processes such as bioethanol ...
Natalia Gutierrez Calle   +2 more
doaj   +1 more source

Transformation Protocol for BL21(DE3) Competent Cells (C2527I) v2 [PDF]

open access: yes, 2020
This transformation protocol is for the C2527I cells. (For the C2527H protocol, see here.)
openaire   +1 more source

Technical Data of Heterologous Expression and Purification of SARS-CoV-2 Proteases Using Escherichia coli System

open access: yesData, 2021
The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the ...
Rafida Razali   +2 more
doaj   +1 more source

Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3) [PDF]

open access: yesGenome Announcements, 2017
ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21 ...
Goffin, Philippe, Dehottay, Philippe
openaire   +2 more sources

PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP

open access: yesJournal of the Indonesian Tropical Animal Agriculture, 2014
This research was aimed at producing a crude intracellular phytase characterized from recombinantbacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+)+pEAS1 into competent E. coli BL21 and E.
A.M.P. Nuhriawangsa   +3 more
doaj   +1 more source

High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. [PDF]

open access: yesPLoS ONE, 2013
Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.
Yao Nie   +6 more
doaj   +1 more source

Effect of Desaturase Gene Overexpression on Fatty Acid Synthesis in Escherichia coli [PDF]

open access: yesShipin Kexue
In order to obtain efficient engineered strains for oil production, the desaturase gene from Bacillus subtilis HB1310, an endophytic bacterium isolated from walnut, was expressed in Escherichia coli BL21 (DE3), the single-gene-expression strains BL21(DE3)
YE Jing, XU Siyuan, ZHANG Qin, QIAN Cheng, CAO Juanjuan, ZHAO Pei
doaj   +1 more source

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