Different vectors used to transform and clone of nonstructural NS1 gene of Influenza B in Escherichia coli [PDF]
Iraqi Journal of Veterinary Sciences, 2019 Flu is a highly contagious and common illness caused by influenza A, B, and C viruses. The aim of the present study was to investigate the transformation and cloning of NS1B gene with pET-32a, pET-32b and pQE-81L in Escherichia coli BL21(DE3) and DH5α ...
Ali Dawood
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Genetic incorporation of D-Lysine into diketoreductase in Escherichia coli cells [PDF]
, 2012 D-Lysine has been genetically introduced into diketoreductase in E. coli cells by utilization of an orthogonal Ph tRNA /Lysyl-tRNA synthetase pair.
Denghuan Yi +4 more
core +2 more sources
Cell Reports, 2015 The well-established E. coli protein production strain C41(DE3) was isolated from the T7 RNA polymerase-based BL21(DE3) strain for its ability to produce difficult recombinant proteins, and it acquired multiple mutations during its isolation.
Susan Schlegel +2 more
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Efflux mediated chlorpyrifos tolerance inEscherichia coliBL21(DE3) [PDF]
, 2020 AbstractBacteria are continually challenged with variety of synthetic chemicals/xenobiotics in their immediate surroundings, including pesticides. Chlorpyrifos is one of the most commonly used organophosphate pesticides in the world. The non-environmental strain ofEscherichia coli,BL21 (DE3) displayed high tolerance to chlorpyrifos but with no ...
Aswathi Aswathi +2 more
openaire +1 more source
High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization [PDF]
, 2015 The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is ...
Fernández Lahore, Marcelo +5 more
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β-Glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus [PDF]
, 2020 A clone encoding carboxymethyl cellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host.
Alessi, Anna M +11 more
core +3 more sources
Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis
Frontiers in Microbiology, 2019 Bacteria-based biotechnology processes are constantly under threat from bacteriophage infection, with phage contamination being a non-neglectable problem for microbial fermentation.
Ping Li +7 more
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Crystallization of the regulatory and effector domains of the key sporulation response regulator Spo0A [PDF]
, 1999 The key response-regulator gene of sporulation, spo0A, has been cloned from Bacillus stearothermophilus and the encoded protein purified. The DNA-binding and phospho-acceptor domains of Spo0A have been prepared by tryptic digestion of the intact protein ...
Barak, I +7 more
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Microbial Cell Factories, 2022 Background Escherichia coli is one of the most important hosts for production of recombinant proteins in biopharmaceutical industry. However, when selecting a suitable production strain, it is often not considered that a lot of different sub-species ...
Florian Mayer +7 more
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Squalene Biosynthesis in Engineered Escherichia Coli
Pharmaceutical Fronts, 2023 Squalene is a natural triterpenoid that is important in medicine, and daily-use chemical and nutraceutical industries. The demand for squalene remains high and constant.
Wei Zhang +3 more
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