Results 41 to 50 of about 101,082 (154)

بهینه‏سازی کدونی و مقایسه‏ی بیان فاکتور رشد عصبی بتا انسانی (β-NGF) بصورت نوترکیب در میزبان‏های باکتریایی( BL21(DE3 و BL21(DE3)pLysS [PDF]

زیست‌شناسی کاربردی, 2017
فاکتور رشد عصبی بتا (β-NGF) برای اولین بار بدلیل نقش حیاتی‌اش در رشد و بقای سلول‏های عصبی مورد مطالعه قرار گرفت. این پروتئین که به خانواده‌ی نوروتروفین‌ها تعلق دارد در درمان بیماری‌های تخریب عصبی نظیر آلزایمر دارای نقش قابل ملاحظه‏ای می‏باشد. بدین منظور،
معصومه رجبی بذل, زهرا حاجی حسن, مونا سهرابی   +2 more
doaj   +1 more source

To Construct an Engineered (S)-Equol Resistant E. coli for in Vitro (S)-Equol Production

Frontiers in Microbiology, 2018
(S)-equol is one of the major metabolites of daidzein that is produced by human and animal gut bacteria. Most of the physiological functions of soybean isoflavones, such as anti-oxidative activity, anti-cancer activity, and cardiovascular protection have
Hailiang Li, Hailiang Li, Hailiang Li, Shaoming Mao, Huahai Chen, Huahai Chen, Liying Zhu, Wei Liu, Xin Wang, Yeshi Yin, Yeshi Yin   +10 more
doaj   +1 more source

Cloning and expression of Plasmodium falciparum lactate dehydrogenase (PfLDH) in Escherichia coli BL21(DE3)

Acta Biochimica Indonesiana, 2019
Background: Immediate and accurate diagnosis of malaria is essential for effective control of this disease. Immunochromatographic based rapid diagnostic tests (RDTs)  are economical, simple to perform, and provide results in a relative short time, can be
Fifi Fitriyah Masduki, Yeni Hotimah, Rosalia Rani, Arsyam Mawardi, Euniche R.P.F. Ramandey, Azzania Fibriani, Sony Suhandono   +6 more
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Optimizing Recombinant Cas9 Expression: Insights from E. coli BL21(DE3) Strains for Enhanced Protein Purification and Genome Editing

Biomedicines
The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, while
Shilpi Agrawal, Made Harumi Padmaswari, Abbey L. Stokes, Daniel Maxenberger, Morgan Reese, Adila Khalil, Christopher E. Nelson   +6 more
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Comparative expression of soluble, active human kinases in specialized bacterial strains.

PLoS ONE, 2022
Kinases act as molecular switches for cellular functions and are involved in multiple human pathogeneses, most notably cancer. There is a continuous need for soluble and active kinases for in-vitro drug discovery and structural biology purposes.
Allison Sunderhaus, Ramsha Imran, Elanzou Enoh, Adesola Adedeji, Taiye Obafemi, May H Abdel Aziz   +5 more
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Mechanistic platform knowledge of concomitant sugar uptake in Escherichia coli BL21(DE3) strains [PDF]

Scientific Reports, 2017
AbstractWhen producing recombinant proteins, the use of Escherichia coli strain BL21(DE3) in combination with the T7-based pET-expression system is often the method of choice. In a recent study we introduced a mechanistic model describing the correlation of the specific glucose uptake rate (qs,glu) and the corresponding maximum specific lactose uptake ...
David J. Wurm, Johanna Hausjell, Sophia Ulonska, Christoph Herwig, Oliver Spadiut   +4 more
openaire   +2 more sources

Construction of probiotic Escherichia coli Nissle 1917 as a next-generation expression platform and its application in therapeutic protein production

Journal of Biological Engineering
Biologics produced in Escherichia coli BL21(DE3) require rigorous removal of lipopolysaccharide (LPS), since even traces can trigger severe immune responses and potentially fatal sequelae in humans.
Chenglin Wang, Lun Luo, Ziqiao Dai, Wantong Zhang, Yi Ma, Jufang Wang   +5 more
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Characterization of Escherichia coli Strains for Novel Production of Plasmodium ovale Lactate Dehydrogenase

Microorganisms
Malaria is one of the most prevalent diseases worldwide with high incidence and mortality. Among the five species that can infect humans, Plasmodium ovale morphologically resembles Plasmodium vivax, resulting in misidentification and confusion in ...
Jae-Won Choi, Sang-Oh Ha, Yeon-Jun Kim, Jun-Seop Shin, Min-Ji Choi, Si-Eun Yu, Junghun Han, Eun-Ji Park, Kyoung Sik Park, Jung Hoon Kang   +9 more
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Protein Expression Using BL21(DE3) (C2527) v2

, 2020
This protocol explains methods for Protein Expression Using BL21(DE3) Competent E. coli (C2527). You can find the protocol for a large-scale Protein Expression here.
openaire   +1 more source

Relationship Between Opacity of Transformed E. coli Colonies and Over-Expression of the Recombinant Transcript

BioTechniques, 1997
Following transformation with recombinant plasmid clones, E. coli BL21(DE3) often produced extremely opaque colonies on a standard semisolid agar plate, as compared with the translucent colonies produced by normal, untransformed bacteria. A standard BL21(
Sailen Barik
doaj   +1 more source