Results 51 to 60 of about 101,082 (154)

Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. [PDF]

PLoS ONE, 2014
Excess acetate has long been an issue for the production of recombinant proteins in E. coli cells. Recently, improvements in acetate tolerance have been achieved through the use of genetic strategies and medium supplementation with certain amino acids ...
Hengwei Wang, Fengqing Wang, Wei Wang, Xueling Yao, Dongzhi Wei, Hairong Cheng, Zixin Deng   +6 more
doaj   +1 more source

The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

Microbial Cell Factories, 2017
Background To optimize the production of membrane and secretory proteins in Escherichia coli, it is critical to harmonize the expression rates of the genes encoding these proteins with the capacity of their biogenesis machineries.
Grietje Kuipers, Alexandros Karyolaimos, Zhe Zhang, Nurzian Ismail, Gianluca Trinco, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier   +7 more
doaj   +1 more source

Activation of the Glutamic Acid-Dependent Acid Resistance System in Escherichia coli BL21(DE3) Leads to Increase of the Fatty Acid Biotransformation Activity. [PDF]

PLoS ONE, 2016
The biosynthesis of carboxylic acids including fatty acids from biomass is central in envisaged biorefinery concepts. The productivities are often, however, low due to product toxicity that hamper whole-cell biocatalyst performance.
Ji-Min Woo, Ji-Won Kim, Ji-Won Song, Lars M Blank, Jin-Byung Park   +4 more
doaj   +1 more source

Recombinant Cystobasidium minutum Aminopeptidase DmpA Efficiently Catalyzes the Production of L-Carnosine

Shipin gongye ke-ji
This experiment investigated the ability of an aminopeptidase gene dmpA from microcyst basidiomycete MCA 4210 (Cystobasidium minutum MCA 4210) to catalyze the synthesis of L-carnosine, and constructed a genetically engineered strain to catalyze the ...
Xiaoxuan SUN, Ruijie ZHANG, Wen SUN, Lingyu CHEN, Xianfeng ZHU, Baoguo ZHANG   +5 more
doaj   +1 more source

Protein Expression Using BL21(DE3) (C2527) v1

, 2014
This is the protocol for Protein Expression Using BL21(DE3) Competent E. coli (C2527). For large scale, please follow this protocol variant.
openaire   +1 more source

High Expression Achievement of Active and Robust Anti-β2 microglobulin Nanobodies via E.coli Hosts Selection

Molecules, 2019
Nanobodies (VHHs) overcome many of the drawbacks of conventional antibodies, and the related technologies represent state-of-the-art and advanced applications in scientific research, pharmaceuticals, and therapies.
Da Li, Fangling Ji, Chundong Huang, Lingyun Jia   +3 more
doaj   +1 more source

The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Biotechnology Reports, 2017
Rare arginine codons AGA and AGG affect the heterologous expression of proteins in Eschericha coli. The tRNAs necessary for protein synthesis are scarce in E. coli strain BL21(DE3) pLysS and plentiful in strain BL21(DE3) CodonPlus −RIL.
Beatriz Aguirre-López, Nallely Cabrera, Marietta Tuena de Gómez-Puyou, Ruy Perez-Montfort, Armando Gómez-Puyou   +4 more
doaj   +1 more source

Expression of Recombinant Sugarcane Streak Mosaic Virus Coat Protein Gene in Escherichia coli

Hayati Journal of Biosciences, 2016
Sugarcane streak mosaic virus (SCSMV) is an important virus causing mosaic disease in sugarcane and transmitted through the cutting cane. Commercial antiserum to detect SCSMV and to monitor the disease development is not available.
Hamdayanty Hamdayanty, Sri Hendrastuti Hidayat, Tri Asmira Damayanti   +2 more
doaj   +1 more source

Metabolic Modification of Escherichia coli BL21 (DE3) to Produce Shinorine

Shinorine, a naturally occurring UV-absorbing compound belonging to the class of mycosporine-like amino acids (MAAs), has attracted considerable attention for its applications in pharmaceuticals, cosmetics, and biomaterials. However, conventional production methods based on extraction from marine organisms are constrained by low yield, limited ...
Wanci Wang, Lingyu Hou, Jiajia Zhao, Yanyu Qiao, Kuncan Wei, Ding Li, Xiyao Liu, Yongguang Jiang   +7 more
openaire   +1 more source

Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3). [PDF]

Microb Cell Fact, 2023
Han P, Fan H, Tong Y.
europepmc   +1 more source