Results 61 to 70 of about 143,695 (288)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Cloning and expression of Plasmodium falciparum lactate dehydrogenase (PfLDH) in Escherichia coli BL21(DE3)

Acta Biochimica Indonesiana, 2019
Background: Immediate and accurate diagnosis of malaria is essential for effective control of this disease. Immunochromatographic based rapid diagnostic tests (RDTs)  are economical, simple to perform, and provide results in a relative short time, can be
Fifi Fitriyah Masduki, Yeni Hotimah, Rosalia Rani, Arsyam Mawardi, Euniche R.P.F. Ramandey, Azzania Fibriani, Sony Suhandono   +6 more
doaj   +1 more source

A eubacterial origin for the human tRNA nucleotidyltransferase? [PDF]

, 2001
tRNA CCA-termini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily.
Aebi M., Altschul S.F., Chen J.Y., Cudny H., Deutscher M.P., Deutscher M.P., Deutscher M.P., Deutscher M.P., Martin G., Raynal L.C., Saitou N., Yue D., Zhu L.   +12 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Optimizing Recombinant Cas9 Expression: Insights from E. coli BL21(DE3) Strains for Enhanced Protein Purification and Genome Editing

Biomedicines
The CRISPR-Cas9 system is a revolutionary tool in genetic engineering, offering unprecedented precision and efficiency in genome editing. Cas9, an enzyme derived from bacteria, is guided by RNA to edit DNA sequences within cells precisely. However, while
Shilpi Agrawal, Made Harumi Padmaswari, Abbey L. Stokes, Daniel Maxenberger, Morgan Reese, Adila Khalil, Christopher E. Nelson   +6 more
doaj   +1 more source

Decrease in gyrase A protein expression in _E. coli_ cells inhibited by antisense ribozymes [PDF]

, 2008
RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. Nucleic acid-based gene interference technologies represent promising strategies for specific inhibition of
Malathi Raghunathan, Shilpakala Sainath Rao   +1 more
core   +1 more source

The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 protein [PDF]

, 2007
Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777.
Abella, M, Barbe, J, Campoy, S, Paytubi, S, Rodriquez, S, White, Malcolm Frederick   +5 more
core   +1 more source

Protein Expression Using BL21(DE3) (C2527) v2

, 2020
This protocol explains methods for Protein Expression Using BL21(DE3) Competent E. coli (C2527). You can find the protocol for a large-scale Protein Expression here.
openaire   +1 more source

Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain [PDF]

ACS Synthetic Biology, 2015
Synthetic auxotrophs are organisms engineered to require the presence of a particular molecule for viability. They have potential applications in biocontainment and enzyme engineering. We show that these organisms can be generated by engineering ligand-dependence into essential genes.
Gabriel, Lopez, J Christopher, Anderson
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source