Results 61 to 70 of about 101,082 (154)

Transformation Protocol for BL21(DE3) Competent Cells (C2527I) v1

, 2015
This transformation protocol is for the C2527I cells. (For the C2527H protocol, see here.)
openaire   +1 more source

Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy. [PDF]

Front Pharmacol, 2023
Li L, Yu B, Lai Y, Shen S, Yan Y, Dong G, Gao X, Cao Y, Ge C, Zhu L, Liu H, Tao S, Yao Z, Li S, Wang X, Hui Q.   +15 more
europepmc   +1 more source

Transformation Protocol for BL21(DE3) Competent Cells (C2527H) v1

, 2015
This transformation protocol is to be performed directly in the C2527H tubes. (For the C2527I protocol, see here.)
openaire   +1 more source

Genetic Engineering of Escherichia coli BL21 (DE3) with a codon-optimized insecticidal toxin complex gene tccZ. [PDF]

Access Microbiol, 2023
Malomane MT, Kondiah K, Serepa-Dlamini MH.   +2 more
europepmc   +1 more source

Expression of Influenza M2e-NP Recombinant Fusion Protein in Escherichia coli BL21 (DE3) and Its Binding to Antibodies. [PDF]

Vaccines (Basel), 2022
Tan MP, Mohamed Alitheen NB, Tan WS, Yap WB.   +3 more
europepmc   +1 more source

Outer membrane protein N expressed in Gram-negative bacterial strain of Escherichia coli BL21 (DE3) Omp8 Rosetta strains under osmoregulation by salts, sugars, and pHs. [PDF]

PLoS One, 2023
Chumjan W, Sangchalee A, Somwang C, Mookda N, Yaikeaw S, Somsakeesit LO.   +5 more
europepmc   +1 more source

Increased cytoplasmic expression of PETase enzymes in E. coli

Microbial Cell Factories
Background Depolymerizing polyethylene terephthalate (PET) plastics using enzymes, such as PETase, offers a sustainable chemical recycling route. To enhance degradation, many groups have sought to engineer PETase for faster catalysis on PET and elevated ...
Luke M. Carter, Chris E. MacFarlane, Samuel P. Karlock, Tridwip Sen, Joel L. Kaar, Jason A. Berberich, Jason T. Boock   +6 more
doaj   +1 more source

Corrigendum: Large-scale preparation of highly stable recombinant human acidic fibroblast growth factor in Escherichia coli BL21(DE3) plysS strain. [PDF]

Front Bioeng Biotechnol, 2022
Yu B, Sun W, Huang Z, Sun G, Li L, Gu J, Zheng M, Li X, Chun C, Hui Q, Wang X.   +10 more
europepmc   +1 more source

Protein Expression Using BL21(DE3), Large Scale (C2527) v2

, 2020
This is the protocol for a large-scale Protein Expression Using BL21(DE3) Competent E. coli cells (C2527).
openaire   +1 more source

Cloning, Expression, and In Vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli [PDF]

Cell Journal, 2013
Objective: The aim of present study was cloning and expression of phiC31 integrase cDNA in a bacterial expression vector. Thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein.Materials and Methods: In this ...
Mohammad Hadi Sekhavati, Mojtaba Tahmoorespur, Kamran Ghaedi, Kianoush Dormiani, Pharm, Mohammad Reza Nassiri, Yahya Khazaie, Mahboubeh Foruzanfar, Morteza Hosseini, Mohammad Hossein Nasr Esfahani   +8 more
doaj