Results 71 to 80 of about 143,695 (288)

Comparative expression of soluble, active human kinases in specialized bacterial strains.

PLoS ONE, 2022
Kinases act as molecular switches for cellular functions and are involved in multiple human pathogeneses, most notably cancer. There is a continuous need for soluble and active kinases for in-vitro drug discovery and structural biology purposes.
Allison Sunderhaus, Ramsha Imran, Elanzou Enoh, Adesola Adedeji, Taiye Obafemi, May H Abdel Aziz   +5 more
doaj   +1 more source

High energy-charged cell factory for heterologous protein synthesis [PDF]

, 2009
Overexpression of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration and, consequently, to up-regulate genes for amino acid and nucleotide ...
Hye Jung Kim, Pil Kim, Sang Yup Lee, Yeong Deok Kwon   +3 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

ADP-dependent Phosphofructokinases in Mesophilic and Thermophilic Methanogenic Archaea [PDF]

, 2001
Phosphofructokinase (PFK) is a key enzyme of the glycolytic pathway in all domains of life. Two related PFKs, ATP-dependent and PPi-dependent PFK, have been distinguished in bacteria and eucarya, as well as in some archaea.
Verhees, C.H., Tuininga, J.E., Kengen, S.W.M., Stams, A.J.M., Oost, J., van der, Vos, W.M., de   +5 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Characterization of Escherichia coli Strains for Novel Production of Plasmodium ovale Lactate Dehydrogenase

Microorganisms
Malaria is one of the most prevalent diseases worldwide with high incidence and mortality. Among the five species that can infect humans, Plasmodium ovale morphologically resembles Plasmodium vivax, resulting in misidentification and confusion in ...
Jae-Won Choi, Sang-Oh Ha, Yeon-Jun Kim, Jun-Seop Shin, Min-Ji Choi, Si-Eun Yu, Junghun Han, Eun-Ji Park, Kyoung Sik Park, Jung Hoon Kang   +9 more
doaj   +1 more source

Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum [PDF]

, 1921
Conjugated bile salt hydrolase (BSH) catalyses the hydrolysis of the amide bond that conjugates bile acids to glycine and to taurine. The BSH enzyme from Bifidobacterium longum was overexpressed in Escherichia coli BL21(DE3), purified and crystallized ...
Kumar, R S, Brannigan, J A, Pundle, A, Prabhune, A, Dodson, G G, Suresh, C G   +5 more
core   +2 more sources

Coacervates Made of Elastin‐Like Polypeptides Fused with Melanocyte‐Stimulating Hormone and Monocyte Chemoattractant Protein Enhance Skin Wound Healing in Spinal Cord‐Injured Mice

Advanced Healthcare Materials, EarlyView.
Pressure skin wounds are frequent complications after spinal cord injury (SCI), with impaired healing due to vascular and immune deficits. Elastin‐like polypeptides (ELP) fused to α‐MSH (MSH‐ELP) or MCP‐1 (MCP‐ELP) are developed and tested on these wounds. The resulting nanoparticles are non‐toxic and bioactive, and they enhance macrophage recruitment,
Suneel Kumar, Dhruv Patel, Greeshma Manjunath, Shashank Madhavan, Ashley Mathew, Dnyaneshwari Rananavare, Jahnavi Shah, Francois Berthiaume   +7 more
wiley   +1 more source

Construction of probiotic Escherichia coli Nissle 1917 as a next-generation expression platform and its application in therapeutic protein production

Journal of Biological Engineering
Biologics produced in Escherichia coli BL21(DE3) require rigorous removal of lipopolysaccharide (LPS), since even traces can trigger severe immune responses and potentially fatal sequelae in humans.
Chenglin Wang, Lun Luo, Ziqiao Dai, Wantong Zhang, Yi Ma, Jufang Wang   +5 more
doaj   +1 more source

Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane [PDF]

, 2015
Lipopolysaccharide (LPS) is a main component of the outer membrane of Gram-negative bacteria, which is essential for the vitality of most Gram-negative bacteria and plays a critical role for drug resistance.
AX Tran, B van den Berg, C Dong, C Whitfield, CC Liu, DJ Sherman, E Freinkman, E Freinkman, EM Hearn, G Malojcic, G Zhang, H Dong, H Hong, H Liu, H Mori, JC Kauer, JC Whitney, JW Chin, KM Schultz, M Grabowicz, MA Khan, MD Suits, N Ruiz, NN Nickerson, P Sperandeo, P Sperandeo, QJ Xiang, R Villa, RE Bishop, RE Bishop, S Okuda, S Okuda, S Qiao, TS Young, Y Gu, Z Wang   +35 more
core   +1 more source