Results 81 to 90 of about 143,695 (288)

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

Relationship Between Opacity of Transformed E. coli Colonies and Over-Expression of the Recombinant Transcript

BioTechniques, 1997
Following transformation with recombinant plasmid clones, E. coli BL21(DE3) often produced extremely opaque colonies on a standard semisolid agar plate, as compared with the translucent colonies produced by normal, untransformed bacteria. A standard BL21(
Sailen Barik
doaj   +1 more source

Expansion of Thaumarchaeota habitat range is correlated with horizontal transfer of ATPase operons. [PDF]

, 2019
Thaumarchaeota are responsible for a significant fraction of ammonia oxidation in the oceans and in soils that range from alkaline to acidic. However, the adaptive mechanisms underpinning their habitat expansion remain poorly understood.
Bartlett, Douglas H, Eloe-Fadrosh, Emiley A, Hall, Richard, Han, Ping, Heiner, Cheryl, Herbold, Craig W, Huang, Li, Ingalls, Anitra E, Ji, Junbin, Jia, Zhongjun, Jung, Man-Young, Li, Meng, Liu, Linmeng, Liu, Yang, Lu, Lu, Martens-Habbena, Willm, Qin, Wei, Ren, Yi, Rhee, Sung-Keun, Stahl, David A, Wagner, Michael, Wang, Baozhan, Yan, Xin, Zheng, Yue, Zhou, Xue   +24 more
core   +2 more sources

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source

The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

Microbial Cell Factories, 2017
Background To optimize the production of membrane and secretory proteins in Escherichia coli, it is critical to harmonize the expression rates of the genes encoding these proteins with the capacity of their biogenesis machineries.
Grietje Kuipers, Alexandros Karyolaimos, Zhe Zhang, Nurzian Ismail, Gianluca Trinco, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier   +7 more
doaj   +1 more source

In Vitro Stability of Phytase from Recombinant Bacteria E. Coli BL21 (DE3) EAS1-AMP [PDF]

, 2011
The objective of the research was to inquire the Km, Vm, activity, intracellular phytase stability exposed to pH variation, temperature variation and protease (pepsin and pancreas) in vitro.
Bachruddin, Z., Nuhriawangsa, A. M. P., SAJIDAN,, Wibowo, A.   +3 more
core  

Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways [PDF]

, 2019
Background The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow, especially at low salinity ...
Argandoña Bertrán, Montserrat, Bernal, Vicente, Borges, Nuno, Castaño Cerezo, Sara, Csonka, Laszlo N., Cánovas, Manuel, Gonçalves, Luís G., Goodner, Bradley W., Nieto Gutiérrez, Joaquín José, Pagán, Juan P., Pastor, José M., Reynolds, Kathryn A., Vargas Macías, Carmen   +12 more
core   +1 more source

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source

Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl‐tRNA Synthetase Boosts Genetic Code Expansion in E. coli

Advanced Science, EarlyView.
ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch, Peter Goettig, Michael A. Nash, Juri Rappsilber, Nediljko Budisa   +4 more
wiley   +1 more source

Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. [PDF]

PLoS ONE, 2014
Excess acetate has long been an issue for the production of recombinant proteins in E. coli cells. Recently, improvements in acetate tolerance have been achieved through the use of genetic strategies and medium supplementation with certain amino acids ...
Hengwei Wang, Fengqing Wang, Wei Wang, Xueling Yao, Dongzhi Wei, Hairong Cheng, Zixin Deng   +6 more
doaj   +1 more source