Results 91 to 100 of about 143,695 (288)
A functional RNase P protein subunit of bacterial origin in some eukaryotes [PDF]
, 2011 RNase P catalyzes 5′-maturation of tRNAs. While bacterial RNase P comprises an RNA catalyst and a protein cofactor, the eukaryotic (nuclear) variant contains an RNA and up to ten proteins, all unrelated to the bacterial protein.
Bernal Bayard, Pilar +5 more
core +1 more source
Protein Expression Using BL21(DE3), Large Scale (C2527) v2
, 2020 This is the protocol for a large-scale Protein Expression Using BL21(DE3) Competent E. coli cells (C2527).
openaire +1 more source
Advanced Science, EarlyView.An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Yushi Liu +4 more
wiley +1 more source
Activation of the Glutamic Acid-Dependent Acid Resistance System in Escherichia coli BL21(DE3) Leads to Increase of the Fatty Acid Biotransformation Activity. [PDF]
PLoS ONE, 2016 The biosynthesis of carboxylic acids including fatty acids from biomass is central in envisaged biorefinery concepts. The productivities are often, however, low due to product toxicity that hamper whole-cell biocatalyst performance.
Ji-Min Woo +4 more
doaj +1 more source
Protein Expression Using BL21(DE3) (C2527) v1
, 2014 This is the protocol for Protein Expression Using BL21(DE3) Competent E. coli (C2527). For large scale, please follow this protocol variant.
openaire +1 more source
Advanced Science, EarlyView.NPM1 selectively sorts specific mRNAs into extracellular vesicles (EVs) by recognizing RNA motifs and forming phase‐separated condensates. These mRNA cargos—including EGFR—are then loaded via multivesicular bodies. This active sorting pathway, validated in EVs derived from both lung cancer cells and patient serum, reveals a specific mechanism for ...
Kaixiang Zhang +8 more
wiley +1 more source
Function of the Signal Peptide and N- and C-terminal Propeptides in the Leucine Aminopeptidase from \u3cem\u3eAeromonas proteolytica\u3c/em\u3e [PDF]
, 2004 The leucine aminopeptidase from Aeromonas proteolytica (also known as Vibrio proteolyticus) (AAP) is a metalloenzyme with broad substrate specificity. The open reading frame (ORF) for AAP encodes a 54 kDa enzyme, however, the extracellular enzyme has a ...
Bzymek, Krzysztof P. +5 more
core +1 more source
Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation [PDF]
, 2015 Figure S1. Physiology of recombinant E. coli BL21(DE3)(pLysS) strains bearing pET-24a. Panel A and B show biomass formation (circles), glucose consumption (squares), acetate formation (triangles), and proline consumption (diamonds) during batch ...
Andreas Schmid +3 more
core +3 more sources
Advanced Science, EarlyView.We identify a Lepidoptera‐conserved testis‐specific arylalkylamine N‐acetyltransferase (LTNAT) that governs male moth fertility via a novel mechanism. LTNAT loss disrupts eupyrene sperm mitochondrial derivatives and impairs apyrene sperm motility, offering a safe molecular target for innovative pesticides and genetic pest control.
Hao Sun +5 more
wiley +1 more source
TRANSFORMASI PLASMID YANG MENGANDUNG GEN merB PADA Escherichia coli BL21(DE3)
PHARMACON, 2019 ABSTRACTDNA transformation is one of the methods for inserting DNA into bacterial cells. The current transformation method is widely used to transfer plasmids containing genetic material. This study aims to evaluate the results of plasmid transformation containing merB gene in Escherichia coli BL21(DE3) bacteria.
Bernadus, Zefanya G +2 more
openaire +3 more sources