Results 111 to 120 of about 101,082 (154)
Cloning and expression of Bacillus licheniformisamyS gene in E. coli BL21 (DE3)
International Journal of Biosciences (IJB), 2014 openaire +1 more source
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Protein Expression and Purification, 2004
Two mutant strains of Escherichia coli BL21(DE3), called C41(DE3) and C43(DE3) and originally described by Miroux and Walker, are frequently used to overcome the toxicity associated with overexpressing recombinant proteins using the bacteriophage T7 RNA polymerase expression system.
Laurence, Dumon-Seignovert +2 more
exaly +3 more sources
Two mutant strains of Escherichia coli BL21(DE3), called C41(DE3) and C43(DE3) and originally described by Miroux and Walker, are frequently used to overcome the toxicity associated with overexpressing recombinant proteins using the bacteriophage T7 RNA polymerase expression system.
Laurence, Dumon-Seignovert +2 more
exaly +3 more sources
Construction of a synthetic YdbK-dependent pyruvate:H2 pathway in Escherichia coli BL21(DE3)
Metabolic Engineering, 2009A synthetic pyruvate:H(2) pathway was constructed in Escherichia coli BL21(DE3) by co-expression of six proteins: E. coli YdbK, Clostridium pasteurianum [4Fe-4S]-ferredoxin, and Clostridium acetobutylicum HydF, HydE, HydG, and HydA. The effect of cofactor addition and host strain on H(2) yield and fermentation product accumulation was studied, together
Patrik Jones, M Kalim Akhtar
exaly +3 more sources
The dynamic changes of glycogen molecular structure in Escherichia coli BL21(DE3)
Carbohydrate Polymers, 2021Diurnal alteration of glycogen molecular structure has been identified in healthy mice. Recently, both fragile (disintegration in dimethyl sulfoxide) and stable (not disintegrating in DMSO) glycogen particles were found in Escherichia coli. However, how glycogen structure changes dynamically in E. coli is not clear.
Mengmeng Wang +9 more
openaire +4 more sources
On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains
Journal of Biotechnology, 2019The CRISPR-Cas9 system is a new tool that has been extensively used for genome editing. The system is composed of a Cas9 endonuclease, which has the function of cleaving DNA at a specific site, and a guide RNA (gRNA), which contains the sequence of the cleavage site that is the target of editing.
Gabriela Pannunzio, Carmignotto +1 more
openaire +2 more sources
Optimized expression and refolding of human keratoepithelin in BL21 (DE3)
Protein Expression and Purification, 2004Keratoepithelin (KE) is an extracellular protein participating in cell adhesion and differentiation. Mutations of the KE gene (on 5q31 in humans) cause deposition of abnormal proteins (amyloid and non-amyloid) in corneal stroma and lead to several corneal dystrophies in humans.
Ching, Yuan +3 more
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Overexpression of a lethal methylase, M.TneDI, in E. coli BL21(DE3)
Biotechnology Letters, 2014A pET-based vector pDH21 expressing the methylase, M.TneDI (recognizing CGCG) from Thermotoga was constructed, and transformed into E. coli BL21(DE3). Despite E. coli BL21(DE3) being McrBC positive, 30 transformants were isolated, which were suspected to be McrBC(-) mutants. The overexpression of M.TneDI was verified by SDS-PAGE analysis.
Hui, Xu, Dongmei, Han, Zhaohui, Xu
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Ethanol effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3)
Applied Microbiology and Biotechnology, 2018A novel method involving ethanol-induced increase in the heterologous recombinant protein expression in E. coli cells was commonly used in recent studies. However, the detailed mechanism of this method is still to be revealed. This work used comparative transcriptomic analysis and numerous experiments to uncover the mechanism of ethanol effects on the ...
Hongchen Zheng +9 more
openaire +2 more sources