The pleiotropic transcriptional regulator NlpR contributes to the modulation of nitrogen metabolism, lipogenesis and triacylglycerol accumulation in oleaginous rhodococci [PDF]
, 2017 The regulatory mechanisms involved in lipogenesis and triacylglycerol (TAG) accumulation are largely unknown in oleaginous rhodococci. In this study a regulatory protein (here called NlpR: Nitrogen lipid Regulator), which contributes to the modulation of
Alvarez, Hector Manuel +4 more
core +1 more source
Identifying Cytokine Motif‐Containing, Immunomodulatory Bacterial Proteins in Human Gut Microbiome
Advanced Science, EarlyView.By building and constructing HMM (Upper left, blue), the authors identify CMCPs in bacteria genomes and CRC related metagenomes and enriched CRC‐related CMCPs (Upper right, blue). They analyze sequence and structural similarity of hits (Lower left, green), test function with engineered EcN delivered to tumors in a mouse tumor model (Lower right, pink ...
Ziyu Wang +12 more
wiley +1 more source
Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions [PDF]
, 2017 This work was supported in part by the European Union, Area NMP.2013.1.1–2: Self-assembly of naturally occurring nanosystems: CellulosomePlus Project number: 604530, and by the EU Seventh Framework Programme (FP7 2007–2013) under the WallTraC project ...
Alves, Victor D +17 more
core +1 more source
Advanced Science, EarlyView.In this study, the orange‐muscle giant abalone (Haliotis gigantea) is used as a model to identify a non‐coding SNP that disrupts the interaction between ITGA8 pre‐mRNA and the splicing factor ILF2, leading to altered ITGA8 splicing. These splicing changes promote carotenoid accumulation in abalone muscle through the regulation of tissue remodeling ...
Xiaohui Wei +17 more
wiley +1 more source
Novel frataxin isoforms may contribute to the pathological mechanism of friedreich ataxia [PDF]
, 2012 This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
Di Zhou +11 more
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Transformation Protocol for BL21(DE3) Competent Cells (C2527H) v1
, 2015 This transformation protocol is to be performed directly in the C2527H tubes. (For the C2527I protocol, see here.)
openaire +1 more source
Nanoscale Spatial Organization of ARC High‐ and Low‐Order Assemblies at Excitatory Synapses
Advanced Science, EarlyView.ARC (Activity‐Regulated Cytoskeleton‐Associated protein) mediates synaptic plasticity by forming nanoscale assemblies in neurons. Using super‐resolution microscopy and time‐resolved anisotropy with targeted tagging, the study reveals low‐order ARC assemblies at synapses colocalizing with AMPARs, semi‐circular structures at endocytic zones, and 60–80 nm
Martina Damenti +13 more
wiley +1 more source
Bioengineering bacterial outer membrane vesicles as delivery system for RNA therapeutics targeted to lung epithelial cytosols [PDF]
, 2020 Intact epithelia lining the airways and alveoli in the lung are essential to maintain lung function. Structural or functional damage of epithelial cells leads in severe diseases, including COPD/emphysema, ibrosis or ALI/ARDS.
Brown, Nicola +5 more
core
CadC-mediated activation of the cadBA promoter in Escherichia coli [PDF]
, 2005 The transcriptional activator CadC in Escherichia coli, a member of the ToxR-like proteins, activates transcription of the cadBA operon encoding the lysine decarboxylase CadA and the lysine-cadaverine antiporter CadB. cadBA is induced under conditions of
Jung, K., Kuper, C.
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Protein Expression Using BL21(DE3), Large Scale (C2527) v1
, 2014 This is the protocol for a large-scale Protein Expression Using BL21(DE3) Competent E. coli cells(C2527).
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