Results 121 to 130 of about 101,082 (154)

Expression and Purification of Tetanus Toxin Fragment C in Escherichia coli BL21(DE3)

Protein & Peptide Letters, 2020
Background: Tetanus is an infectious disease caused by Clostridium secreting tetanus toxin in anaerobic environment. The fragment C of Tetanus toxin (TTc) has been widely studied as a candidate vaccine to replace the existing tetanus toxoid vaccine. Objective: In this study, we established a simple method to purify recombinant protein TTc with ion ...
Pengdi, Chai, Xiuying, Pu, Jianqiang, Li, Xiaoyu, Xia, Jun, Ge, Amiao, Luo, Hui, Su, Weijie, Zhang, Jianzhong, Ma   +8 more
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Genome Sequences of Escherichia coli B strains REL606 and BL21(DE3)

Journal of Molecular Biology, 2009
Escherichia coli K-12 and B have been the subjects of classical experiments from which much of our understanding of molecular genetics has emerged. We present here complete genome sequences of two E. coli B strains, REL606, used in a long-term evolution experiment, and BL21(DE3), widely used to express recombinant proteins.
Jeong, H Jeong, Haeyoung, Barbe, V Barbe, Valerie, Lee, CH Lee, Choong Hoon, Vallenet, D Vallenet, David, Yu, DS Yu, Dong Su, Choi, SH Choi, Sang-Haeng, Couloux, A Couloux, Arnaud, Lee, SW Lee, Seung-Won, Yoon, SH Yoon, Sung Ho, Cattolico, L Cattolico, Laurence, Hur, CG Hur, Cheol-Goo, Park, HS Park, Hong-Seog, Segurens, B Segurens, Beatrice, Kim, SC Kim, Sun-Chang, Oh, TK Oh, Tae Kwang, Lenski, RE Lenski, Richard E., Studier, FW Studier, F. William, Daegelen, P Daegelen, Patrick, Kim, JF Kim, Jihyun F.   +18 more
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A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)

2021
Continuous processing allows to maximize space-time yields and is implemented in many industrial branches. However, in manufacturing of value added compounds produced with microbial hosts, continuous processing is not state-of-the-art yet. This is because fluctuating productivity causes unwanted process deviations.
Julian, Kopp, Oliver, Spadiut
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Discovery of novel feruloyl esterase activity of BioH in Escherichia coli BL21(DE3)

Biotechnology Letters, 2016
To characterize a novel feruloyl esterase from Escherichia coli BL21 DE3.The gene encoding BioH was cloned and overexpressed in E. coli. The protein was purified and its catalytic activity was assessed. BioH exhibited feruloyl esterase activity toward a broad range of substrates, and the corresponding kinetic constants for the methyl ferulate, ethyl ...
Le, Kang, Yajun, Bai, Yujie, Cai, Xiaohui, Zheng   +3 more
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Multiple Stressor-Induced Proteome Responses of Escherichia coli BL21(DE3)

Journal of Proteome Research, 2008
Through 2-DE based quantitative proteomics, the dynamic characteristics of overall proteome profiles of Escherichia coli BL21(DE3) were systematically analyzed in the presence of four different stressors. Dithiothreitol and 2-hydroxyethyl disulfide are a reducing and an oxidizing agent, respectively, which disturb the redox balance in cytoplasm, while ...
Kyung-Yeon, Han, Jin-Seung, Park, Hyuk-Seong, Seo, Keum-Young, Ahn, Jeewon, Lee   +4 more
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Expression and characterization of soybean seed coat peroxidase inEscherichia coliBL21(DE3)

Preparative Biochemistry & Biotechnology, 2017
Soybean seed coat peroxidase (SBP) is a valuable enzyme having a broad variety of applications in analytical chemistry, biochemistry, and food processing. In the present study, the sscp gene (Gene ID: 548068) was optimized based on the preferred codon usage of Escherichia coli, synthesized, and expressed in E. coli BL21(DE3).
Changqing, Liu, Kai, Zheng, Ying, Xu, Lacmata Tamekou, Stephen, Jiming, Wang, Hongwei, Zhao, Tongqing, Yue, Rui, Nian, Haibo, Zhang, Mo, Xian, Huizhou, Liu   +10 more
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[Cloning and expression of caiDE genes in E. coli BL21(DE3)].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2004
The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2.
J, Jiang, S, Duan, H, Zhang
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Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): ammonia effects

Applied Microbiology and Biotechnology, 2009
In shake flasks, good oxygen supply tended to decrease rtPA expression in media containing only yeast extract and tryptone, while oxygen limitation would increase rtPA synthesis in the same medium. Our data showed that though the drop of rtPA expression in the 20-ml cultures of LBG or 2YTG was accompanied with a severe acetate accumulation, it was ...
Hengwei, Wang, Fengqing, Wang, Dongzhi, Wei   +2 more
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Metabolic characteristics of an isocitrate dehydrogenase defective derivative of escherichia coli BL21(DE3)

Biotechnology and Bioengineering, 2003
AbstractA 7.8 kb fragment containing isocitrate dehydrogenase (ICDH) gene and its flanking regions was cloned from Escherichia coli BL21(DE3) and sequenced. Unlike the case of the K‐12 strain, the e14 element was not found. The nucleotide divergence between these two strains was about 2%. Using the cloned fragment, ICDH defective mutant strain, MA1935,
Miho, Aoshima, Masaharu, Ishii, Akihiko, Yamagishi, Tairo, Oshima, Yasuo, Igarashi   +4 more
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Escherichia coli BL21(DE3) chromosome contains a group II capsular gene cluster

Gene, 2006
During our study of de novo synthesis of Escherichia coli K1 capsular polysaccharides, we found that E. coli BL21(DE3) has a capsular gene cluster, similar to those of group II capsular E. coli strains. Analysis of the nucleotide sequence of the E. coli BL21(DE3) gene cluster showed homologues to all group II regions 1 and 3 genes and the presence of ...
Ekaterina N, Andreishcheva, Willie F, Vann   +1 more
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