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Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3)
Biotechnology Letters, 2011Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70%. In this study we investigate the metabolic consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO(2) yields and metabolic fluxes could be observed between the ...
Hendrik, Waegeman +4 more
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2019
The E. coli strain BL21 (DE3) is widely applied for the synthesis of recombinant products using the bacteriophage T7 polymerase-base expression systems. The level of target protein production in E. coli cells has been recently shown to depend on the type of carbon source in the growth media and the producer metabolic properties, especially by acetate ...
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The E. coli strain BL21 (DE3) is widely applied for the synthesis of recombinant products using the bacteriophage T7 polymerase-base expression systems. The level of target protein production in E. coli cells has been recently shown to depend on the type of carbon source in the growth media and the producer metabolic properties, especially by acetate ...
openaire +1 more source
[Fusion expression and identification of angiostatin and endostatin in E. coli BL21(DE3)].
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 2007Angiostatin(AS) and endostatin(ES) are both potent endogenous angiogenesis inhibitors, and the combination of AS and ES has been shown to have synergistic antiangiogenic effects. Here we report the fusion protein AS-ES expressed in E. coli which has antiangiogenic effects.
Xiaoyan, Mao +4 more
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[Expression and assembly of chimeric flagellins in Escherichia coli BL21(DE3) and Salmonella].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2018Flagellin can be expressed in monomeric or polymeric form based on assembly. The difference of these two forms of flagellin is less studied. In this experiment, recombinant plasmid pET-fliC/M2e2 was transferred into Escherichia coli BL21(DE3) and Salmonella SL5928 to express chimeric flagellin, mfliC/M and pfliC/M, respectively, and then their assembly
Maozhi, Hu +6 more
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Overexpression of Keratinase Candidate from Bacillus velezensis LPL061 in Escherichia coli BL21(DE3)
Enzyme and Microbial TechnologyProteases have diverse industrial applications including keratinase, which degrade keratin-rich substrates, such as hair, nails, feathers, and skin. This study aimed to express a protease of Bacillus velezensis LPL061 (WP_003155195.1) in Escherichia coli BL21(DE3) and to evaluate its keratinolytic as a new source of keratinase. Two gene constructs were
Desi Sagita +6 more
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Gezielte Optimierung von Escherichia coli BL21(DE3)
BIOspektrum, 2013Stephan Noll +7 more
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