Results 131 to 140 of about 143,695 (288)

Bacillus subtilis regulatory protein GerE [PDF]

, 1998
GerE is the latest-acting of a series of factors which regulate gene expression in the mother cell during sporulation in Bacillus. The gene encoding GerE has been cloned from B. subtilis and overexpressed in Escherichia coli.
Brannigan, J A, Ducros, V M A, Lewis, R J, Wilkinson, A J   +3 more
core   +2 more sources

G4‐Ligand‐Directed PROTACs Unveil DR1 as a Novel Ligand‐Co‐Binding G4‐Protein and Reshape G4‐Dependent Transcription

Advanced Science, EarlyView.
G4‐binding proteins (G4BPs) that specifically co‐bind G4s in the presence of small‐molecule ligands represent a critical but unexplored class of proteins. We introduce G4‐Ligand‐Directed PROTACs (G4L‐TACs), a chemical platform that couples G4 ligands (PDS) to E3 recruiters to selectively degrade these ligand‐co‐binding G4BPs.
Mao‐Lin Li, Shu‐Min Xu, Xin‐Chen Jiang, Le‐Tian Dai, Yu‐Tao Hu, Kai‐Bo Wang, Jia‐Heng Tan, Zhi‐Shu Huang, Shuo‐Bin Chen   +8 more
wiley   +1 more source

Structural analysis of Salmonella enterica effector protein SopD [PDF]

, 2004
Salmonella outer protein D (SopD) is a type III secreted virulence effector protein from Salmonella enterica. Full-length SopD and SopD lacking 16 amino acids at the N-terminus (SopDDeltaN) have been expressed as fusions with GST in Escherichia coli ...
Bagby, S, Galyov, E E, Gill, A C, Philippe, D L, Upadhyay, A, van den Elsen, J M H, Williams, C, Wood, M W   +7 more
core   +1 more source

Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)

RSC Advances, 2017
Cadmium resistance and adsorption genecapBfromEscherichia coliBL21 (DE3).
Weitong Qin, Xiaoqing Liu, Xiaoxia Yu, Xiaoyu Chu, Jian Tian, Ningfeng Wu   +5 more
openaire   +2 more sources

Introgressed Variation in TaMYB7‐A1 Drives Graded Dormancy and Climate‐Adaptive Pre‐Harvest Sprouting Resistance in Wheat

Advanced Science, EarlyView.
TaMYB7‐A1 directly activates TaABI5 to enhance ABA signaling and regulate ABA‐GA homeostasis, enforcing seed dormancy. Its superior allele, derived from wild einkorn introgression, harbors a MITE insertion that elevates expression and two amino acid substitutions that enhance transcriptional activity, collectively generating graded PHS resistance for ...
Hao Wang, Dongzhi Wang, Yafei Guo, Qi Zheng, Min Zhang, James Simmonds, Jian Hou, Xuemei Liu, Xuelei Lin, Xiaomin Bie, Xiansheng Zhang, Xiaohui Li, Yuan Chen, Xueyong Zhang, Cristobal Uauy, Fei Lu, Chengcai Chu, Zhixi Tian, Jun Xiao   +18 more
wiley   +1 more source

Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3). [PDF]

Microb Cell Fact, 2023
Han P, Fan H, Tong Y.
europepmc   +1 more source

Biochemical characterization of the POM-1 metallo-β-lactamase from Pseudomonas otitidis [PDF]

, 2014
The POM-1 metallo--lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and ...
Borgianni, L, Chong, Y, De Luca, F, Docquier, J, Rossolini, G M, Thaller, MC   +5 more
core   +1 more source

CRISPR/Cas13a: Compensatory Target Activation Mechanism

Advanced Science, EarlyView.
CRISPR/Cas13a‐CTAM as a novel compensatory target activation mechanism that enables synergistic activation of Cas13a via two independently editable short RNA effectors. This dual‐effector system maintains enzymatic activity comparable to traditional single‐effector while significantly enhancing flexibility, sensitivity, and application scope.
Bowen Jiang, Tenghua Zhang, Yao Lu, Shujun Zhou, Wenjing Xiao, Shuming Pan, Nan Shi, Yan Sheng, Jiaming Hu   +8 more
wiley   +1 more source

Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy. [PDF]

Front Pharmacol, 2023
Li L, Yu B, Lai Y, Shen S, Yan Y, Dong G, Gao X, Cao Y, Ge C, Zhu L, Liu H, Tao S, Yao Z, Li S, Wang X, Hui Q.   +15 more
europepmc   +1 more source

Engineering an Optogenetic pH‐Modulator in Bacteria

Advanced Science, EarlyView.
ABSTRACT Cells in many naturally occurring organisms routinely cooperate to control their extracellular pH in a dynamic and reversible manner, but this capability has been underexplored in synthetic biology. Here, we sought to engineer a microbial system that switches between two states —high and low extracellular pH— with minimal human intervention ...
Jenevieve Kuang, Olivia J. Armendarez, Wei‐Ting Chang, Matthew M. Hausladen, Shanna Bonanno, Daniel J. Wilson, Neel S. Joshi, Leila F. Deravi   +7 more
wiley   +1 more source