Results 141 to 150 of about 143,695 (288)

Genetic Engineering of Escherichia coli BL21 (DE3) with a codon-optimized insecticidal toxin complex gene tccZ. [PDF]

Access Microbiol, 2023
Malomane MT, Kondiah K, Serepa-Dlamini MH.   +2 more
europepmc   +1 more source

Programmable Semi‐Interpenetrating Living Materials With Robust Stability for Versatile Bioremediation and Biotherapeutics

Advanced Science, EarlyView.
A semi‐interpenetrating engineered living material (sIHSELM), fabricated via thermosensitive hydroxybutyl chitosan and covalent protein self‐assembly, demonstrates exceptional mechanical properties and environmental stability. Shielding bacteria from harsh conditions, sIHSELM exhibits remarkable efficacy in ulcerative colitis treatment and pollutants ...
Zixian Bao, Shengfeng Yang, Dandan Hu, Jiezheng Liu, Bo Jiang, Xinyue Sui, Qingsheng Qi, Lai Li, Guang Zhao   +8 more
wiley   +1 more source

Plant‐Derived Melatonin Inhibits Bacterial Virulence via CpxA/R Two‐Component System

Advanced Science, EarlyView.
Plant‐derived melatonin is sensed by CpxAE48/T51, which inhibits the phosphorylation cascade transmission from CpxAH240 to CpxRD52, resulting in the inhibition of DNA‐binding capacity of CpxR and subsequent T3SS genes expression in Pst DC3000. ABSTRACT In defending against pathogens, plants deploy diverse secondary metabolites and signaling molecules ...
Jin‐Wei Wei, Wei Liu, Bili Cao, Dan Zhao, Chengqiang Wang, Xin Hou, Tangyuan Ning, Biao Gong   +7 more
wiley   +1 more source

Expression of Influenza M2e-NP Recombinant Fusion Protein in Escherichia coli BL21 (DE3) and Its Binding to Antibodies. [PDF]

Vaccines (Basel), 2022
Tan MP, Mohamed Alitheen NB, Tan WS, Yap WB.   +3 more
europepmc   +1 more source

A Novel CsYABBY3‐CsAS1 Feedback Loop Coordinates Trichome Differentiation and Cannabinoid Biosynthesis in Cannabis sativa L.

Advanced Science, EarlyView.
A novel transcriptional module involving CsYABBY3 and CsAS1 is identified to regulate cannabinoid biosynthesis and trichome development. These factors mutually activate each other and form a protein complex via a conserved residue, acting synergistically to amplify metabolic flux through a coordinate feed‐forward mechanism.
Xuewen Zhu, Yaolei Mi, Xue Cao, Weiqiang Chen, Pucheng Fan, Jing Wang, Yiming Zhang, Wei Yang, Huihua Wan, Shanshan Chen, Xiangxiao Meng, Jun Li, Shuo Shen, Mingkun Huang, Xiaoyu Zhang, Ming Luo, Shilin Chen, Zhichao Xu, Wei Sun   +18 more
wiley   +1 more source

Outer membrane protein N expressed in Gram-negative bacterial strain of Escherichia coli BL21 (DE3) Omp8 Rosetta strains under osmoregulation by salts, sugars, and pHs. [PDF]

PLoS One, 2023
Chumjan W, Sangchalee A, Somwang C, Mookda N, Yaikeaw S, Somsakeesit LO.   +5 more
europepmc   +1 more source

Enzymatic Basis for the Oxidative Branch of Aromatic Amino Acid Fermentation Leading to p‐cresol Formation

Advanced Science, EarlyView.
In anaerobic bacteria, tyrosine serves as an electron donor in the oxidative Stickland fermentation branch, generating p‐hydroxyphenylacetate, ATP and reduced ferredoxin for decarboxylation into p‐cresol. ABSTRACT The phenolic metabolite p‐cresol is a byproduct of tyrosine fermentation by certain strictly anaerobic bacteria, including the human gut ...
Li Jiang, Yifeng Wei, Xumei Liu, Dazhi Liu, Zhenyu Liu, Yang Tong, Jinyu Yin, Ankanahalli N. Nanjaraj Urs, Meining Xing, Mark A. Harrison, Chuyuan Zhang, Sheng Yang, Yunzi Luo, Ee Lui Ang, Huimin Zhao, Yan Zhang   +15 more
wiley   +1 more source

Merging Biocatalysis and Chemocatalysis in Flow: State‐of‐the‐Art and Future Directions for Sustainable Synthesis

Angewandte Chemie, EarlyView.
This review highlights recent advances in integrating biocatalysis and chemocatalysis in continuous flow to create streamlined, sustainable processes. It examines chemo‐enzymatic cascades combining at least one enzymatic and one chemical step, discusses challenges such as enzyme immobilization, leaching, and reactor clogging, and presents solutions ...
Petros Siasiaridis, Matteo Damian, Francesco G. Mutti   +2 more
wiley   +2 more sources

Increased cytoplasmic expression of PETase enzymes in E. coli

Microbial Cell Factories
Background Depolymerizing polyethylene terephthalate (PET) plastics using enzymes, such as PETase, offers a sustainable chemical recycling route. To enhance degradation, many groups have sought to engineer PETase for faster catalysis on PET and elevated ...
Luke M. Carter, Chris E. MacFarlane, Samuel P. Karlock, Tridwip Sen, Joel L. Kaar, Jason A. Berberich, Jason T. Boock   +6 more
doaj   +1 more source

Corrigendum: Large-scale preparation of highly stable recombinant human acidic fibroblast growth factor in Escherichia coli BL21(DE3) plysS strain. [PDF]

Front Bioeng Biotechnol, 2022
Yu B, Sun W, Huang Z, Sun G, Li L, Gu J, Zheng M, Li X, Chun C, Hui Q, Wang X.   +10 more
europepmc   +1 more source