Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone [PDF]
, 2013 The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of
Altschul +30 more
core +1 more source
Non-conventional gas phase remediation of volatile halogenated compounds by dehydrated bacteria [PDF]
, 2009 Traditional biological removal processes are limited by the low solubility of halogenated compounds in aqueous media. A new technology appears very suitable for the remediation of these volatile organic compounds (VOCs). Solid/gas bio-catalysis applied
Erable, Benjamin +3 more
core +2 more sources
Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability [PDF]
, 2006 Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9 ...
Erable, Benjamin +4 more
core +4 more sources
High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator. [PDF]
PLoS ONE, 2013 Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry.
Yao Nie +6 more
doaj +1 more source
Flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications [PDF]
, 2014 External flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. These enzymes are involved in a great
Bianchi, Dario Alejandro +2 more
core +2 more sources
Enhanced Expression of Recombinant Activin A in Escherichia coli by Optimization of Induction Parameters [PDF]
Journal of Sciences, Islamic Republic of Iran, 2018 Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector.
Z. Hajihassan, N. Biroonro
doaj +1 more source
Data, 2021 The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the ...
Rafida Razali +2 more
doaj +1 more source
In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6 [PDF]
, 2009 Enzymes of the AlkB and CYP153 families catalyze the first step in the catabolism of medium-chain-length alkanes, selective oxidation of the alkane to the 1-alkanol, and enable their host organisms to utilize alkanes as carbon sources.
Arnold, Frances H. +3 more
core +3 more sources
Transformation Protocol for BL21(DE3) Competent Cells (C2527I) v2 [PDF]
, 2020 This transformation protocol is for the C2527I cells. (For the C2527H protocol, see here.)
openaire +1 more source
Effect of Desaturase Gene Overexpression on Fatty Acid Synthesis in Escherichia coli [PDF]
Shipin KexueIn order to obtain efficient engineered strains for oil production, the desaturase gene from Bacillus subtilis HB1310, an endophytic bacterium isolated from walnut, was expressed in Escherichia coli BL21 (DE3), the single-gene-expression strains BL21(DE3)
YE Jing, XU Siyuan, ZHANG Qin, QIAN Cheng, CAO Juanjuan, ZHAO Pei
doaj +1 more source