Results 31 to 40 of about 101,082 (154)
Enhanced Expression of Recombinant Activin A in Escherichia coli by Optimization of Induction Parameters [PDF]
Journal of Sciences, Islamic Republic of Iran, 2018 Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector.
Z. Hajihassan, N. Biroonro
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Synthetic Auxotrophs with Ligand-Dependent Essential Genes for a BL21(DE3) Biosafety Strain [PDF]
ACS Synthetic Biology, 2015 Synthetic auxotrophs are organisms engineered to require the presence of a particular molecule for viability. They have potential applications in biocontainment and enzyme engineering. We show that these organisms can be generated by engineering ligand-dependence into essential genes.
Gabriel, Lopez, J Christopher, Anderson
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Cell Reports, 2015 The well-established E. coli protein production strain C41(DE3) was isolated from the T7 RNA polymerase-based BL21(DE3) strain for its ability to produce difficult recombinant proteins, and it acquired multiple mutations during its isolation.
Susan Schlegel +2 more
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Different vectors used to transform and clone of nonstructural NS1 gene of Influenza B in Escherichia coli [PDF]
Iraqi Journal of Veterinary Sciences, 2019 Flu is a highly contagious and common illness caused by influenza A, B, and C viruses. The aim of the present study was to investigate the transformation and cloning of NS1B gene with pET-32a, pET-32b and pQE-81L in Escherichia coli BL21(DE3) and DH5α ...
Ali Dawood
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Reduction of the sulfoxide in glucoraphanin and sulforaphane byE. coli VL11 and BL21 (DE3)
Songklanakarin Journal of Science and Technology (SJST), 2016 Songklanakarin Journal of Science and Technology (SJST), 38 ...
Vijitra Luang-In +1 more
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Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis
Frontiers in Microbiology, 2019 Bacteria-based biotechnology processes are constantly under threat from bacteriophage infection, with phage contamination being a non-neglectable problem for microbial fermentation.
Ping Li +7 more
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Microbial Cell Factories, 2022 Background Escherichia coli is one of the most important hosts for production of recombinant proteins in biopharmaceutical industry. However, when selecting a suitable production strain, it is often not considered that a lot of different sub-species ...
Florian Mayer +7 more
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Squalene Biosynthesis in Engineered Escherichia Coli
Pharmaceutical Fronts, 2023 Squalene is a natural triterpenoid that is important in medicine, and daily-use chemical and nutraceutical industries. The demand for squalene remains high and constant.
Wei Zhang +3 more
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Production and purification of constructed recombinant hirudin in BL21(DE3) strain
Journal of Biomedical Engineering and Informatics, 2017 Background/Objective: Hirudin, an extract from the leech, has powerful antithrombin activity affecting the blood coagulation pathway, it is the most potent natural inhibitor of thrombin, it binds thrombin with high affinity, so, the aim of this study was to build hirudin gene by overlapping extension PCR then cloning and expression in BL21(DE3) strain ...
Adnan I. Al-Badran, Sabaa Ali Al-Fadal
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Generic Model Control Applied to E. coli BL21(DE3) Fed-Batch Cultures [PDF]
Processes, 2020 This work proposes a Generic Model Control (GMC) strategy to regulate biomass growth in fed-batch cultures of Escherichia coli BL21(DE3). The control law is established using a previously validated mechanistic model based on the overflow metabolism paradigm.
Merouane Abadli +4 more
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