E. coli bl21 de3 - Open Access .click

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Transferring [NiFe] hydrogenase gene from Rhodopeseudomonas palustris into E. coli BL21(DE3) for improving hydrogen production

International Journal of Hydrogen Energy, 2015
Abstract A recombinant plasmid pETD-SL was constructed to analyze the effect of hydrogen production by [NiFe] hydrogenase gene isolated from Rhodopeseudomonas palustris . A two-promoter vector pETDuet-1 was used to construct pETD-SL which contains hup S and hup L gene, and then pETD-SL was transferred into recombinant Escherichia coli BL21(DE3 ...
Peng Zhou +4 more
openaire +1 more source

Predictor–Response Analysis of Fiber Optic Enzymatic Biosensors Constructed with Nonmodified E. coli BL21 (DE3) pGELAF+ Sensing 1,2-Dichloroethane

Applied Biochemistry and Biotechnology, 2015
Biosensor technology can lack methods to iteratively validate system outputs (i.e., signals) concomitantly with the development of mathematical models. We evaluated a nonmodified fiber optic enzymatic biosensor (FOEB-Escherichia coli BL21 (DE3) pGELAF+) sensing dichloroethane with a predictor-response statistical form.
Cory D, Jensen, Cord, Müeller
openaire +2 more sources

Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3)

Materials Today: Proceedings, 2019
Abstract Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E.
Rafidah Mohamed Ali Hanapiah +4 more
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Cloning and expression of staphylokinase-streptokinase recombinant protein in E. coli BL21(DE3)