Results 11 to 20 of about 1,377,203 (295)

Genetic analysis of the E2 transactivation domain dimerization interface from bovine papillomavirus type 1 [PDF]

Virology, 2013
The bovine papillomavirus type 1 (BPV1) E2 protein binds as a dimer to the viral genome to promote its transcription, replication and maintenance in keratinocytes. Although BPV1 E2 dimerizes primarily through its DNA-binding domain, it was shown previously that its transactivation domain (TAD) can also dimerize in vitro through formation of a disulfide
Gagnon, David, Sénéchal, Hélène, D’Abramo, Claudia M., Alvarez, Jennifer, McBride, Alison A., Archambault, Jacques   +5 more
openaire   +3 more sources

Versatile vector tools for efficient protein screening across multiple expression systems. [PDF]

FEBS Open Bio
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhu Z, Liu Y, Qin L, Yu F, Wang Q, Liu Y.   +5 more
europepmc   +2 more sources

The UBE2/E2 ubiquitin-conjugating enzyme family at the interface of tumor biology and antitumor immunity: mechanisms, biomarkers, and therapeutic opportunities

Frontiers in Immunology
While the catalytic role of ubiquitin-conjugating (UBE2/E2) enzymes within the ubiquitin–proteasome system is well established, their substrate-specifying functions in cancer, particularly at the tumor–immune interface, remain comparatively underexplored.
Hai Zhao, Jiaxin Yang, Fan Yang
doaj   +2 more sources

Inhibitors of apoptosis: expression and regulation in the endometrium during the estrous cycle and at the maternal-conceptus interface during pregnancy in pigs [PDF]

Animal Bioscience, 2022
Objective Caspase-mediated apoptosis plays a crucial role in the regulation of endometrial and placental function in females. Caspase activity is tightly controlled by members of the inhibitors of apoptosis proteins (IAPs) family. However, the expression
Inkyu Yoo, Wonchul Jung, Soohyung Lee, Yugyeong Cheon, Hakhyun Ka   +4 more
doaj   +1 more source

The Binding Interface between an E2 (UBC9) and a Ubiquitin Homologue (UBL1) [PDF]

Journal of Biological Chemistry, 1999
Human UBC9 is a member of the E2 (ubiquitin conjugation enzyme) family of proteins. Instead of conjugating to ubiquitin, it conjugates with a ubiquitin homologue UBL1 (also known as SUMO-1, GMP1, SMTP3, PIC1, and sentrin). UBC9 has been shown to be involved in cell cycle regulation, DNA repair, and p53-dependent processes. The binding interfaces of the
Q, Liu, C, Jin, X, Liao, Z, Shen, D J, Chen, Y, Chen   +5 more
openaire   +2 more sources

Estrogen-sensitive activation of SGK1 induces M2 macrophages with anti-inflammatory properties and a Th2 response at the maternal–fetal interface

Reproductive Biology and Endocrinology, 2023
Background Decidual macrophages participate in immune regulation at the maternal–fetal interface. Abnormal M1/M2 polarization of decidual macrophages might predispose immune maladaptation in recurrent pregnancy loss (RPL).
Yiyun Lou, Zhujing Fu, Ye Tian, Minhao Hu, Qijing Wang, Yuanyuan Zhou, Ning Wang, Qin Zhang, Fan Jin   +8 more
doaj   +1 more source

Crystal Structure of a Ube2S-Ubiquitin Conjugate. [PDF]

PLoS ONE, 2016
Protein ubiquitination occurs through the sequential formation and reorganization of specific protein-protein interfaces. Ubiquitin-conjugating (E2) enzymes, such as Ube2S, catalyze the formation of an isopeptide linkage between the C-terminus of a ...
Sonja Lorenz, Moitrayee Bhattacharyya, Christian Feiler, Michael Rape, John Kuriyan   +4 more
doaj   +1 more source

Loop 7 of E2 enzymes: an ancestral conserved functional motif involved in the E2-mediated steps of the ubiquitination cascade. [PDF]

PLoS ONE, 2012
The ubiquitin (Ub) system controls almost every aspect of eukaryotic cell biology. Protein ubiquitination depends on the sequential action of three classes of enzymes (E1, E2 and E3).
Elena Papaleo, Nicola Casiraghi, Alberto Arrigoni, Marco Vanoni, Paola Coccetti, Luca De Gioia   +5 more
doaj   +1 more source

Probing the antigenicity of hepatitis C virus envelope glycoprotein complex by high-throughput mutagenesis. [PDF]

PLoS Pathogens, 2017
The hepatitis C virus (HCV) envelope glycoproteins E1 and E2 form a non-covalently linked heterodimer on the viral surface that mediates viral entry. E1, E2 and the heterodimer complex E1E2 are candidate vaccine antigens, but are technically challenging ...
Radhika Gopal, Kelli Jackson, Netanel Tzarum, Leopold Kong, Andrew Ettenger, Johnathan Guest, Jennifer M Pfaff, Trevor Barnes, Andrew Honda, Erick Giang, Edgar Davidson, Ian A Wilson, Benjamin J Doranz, Mansun Law   +13 more
doaj   +1 more source

Human Proteome-scale Structural Modeling of E2–E3 Interactions Exploiting Interface Motifs [PDF]

Journal of Proteome Research, 2012
Ubiquitination is crucial for many cellular processes such as protein degradation, DNA repair, transcription regulation, and cell signaling. Ubiquitin attachment takes place via a sequential enzymatic cascade involving ubiquitin activation (by E1 enzymes), ubiquitin conjugation (by E2 enzymes), and ubiquitin substrate tagging (by E3 enzymes).
Kar, Gozde, Keskin, Ozlem, Nussinov, Ruth, Gursoy, Attila   +3 more
openaire   +3 more sources