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ABSTRACT: The effect of pulsed electric fields (PEF) on polygalacturonase (PG) activity in an aqueous solution of a commercial enzyme preparation was studied. Monopolar square wave pulses of 4 μs were delivered to the solution, which circulated through tubular chambers with 2 stainless steel electrodes in ...
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AbstractChromatographically purified endopolygalacturonase (PG) from Aspergillus niger was deglycosylated with N‐glycosidase F (PNGase F) and characterized by means of sodium dodecyl sulfate (SDS)‐electrophoresis, polyacrylamide gel electrophoresis (PAGE) without denaturing agents, isoelectric focusing (IEF) and lectin affino‐blotting. The results show
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