Results 121 to 130 of about 4,853,088 (263)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Enhancing bovine immune, antioxidant and anti-inflammatory responses with vitamins, rumen-protected amino acids, and trace minerals to prevent periparturient mastitis

Frontiers in Immunology
Mastitis, the inflammatory condition of mammary glands, has been closely associated with immune suppression and imbalances between antioxidants and free radicals in cattle.
Muhammad Zahoor Khan, Bingjian Huang, Bingjian Huang, Xiyan Kou, Yinghui Chen, Huili Liang, Qudrat Ullah, Ibrar Muhammad Khan, Adnan Khan, Wenqiong Chai, Changfa Wang   +10 more
doaj   +1 more source

Programmed Interfacial Phase Separation for Spatiotemporal Fungicide Delivery via Tunable Micro/Nano Architectures

Advanced Science, EarlyView.
ABSTRACT Translating dynamic liquid‐liquid phase separation into robust solid‐state architectures remains a challenge in materials engineering. Here, we report a composition‐driven interfacial assembly strategy that couples supramolecular coacervation with metal‐phenolic coordination to engineer tunable fungicide carriers.
Rui Zhang, Tongfang Jing, Leiming He, Kaidi Cui, Yuying Song, Xuewei Mao, Lidong Cao, Lin Zhou   +7 more
wiley   +1 more source

Establishment and evaluation of hematological and serum biochemical reference intervals in Chinese local donkey populations

BMC Veterinary Research
Background Reliable interpretation of hematological and serum biochemical test results in donkeys benefits from the use of population- and management-system-adapted reference intervals (RIs). However, standardized and CLSI-compliant RIs for Chinese local
Qifei Zhu, Muhammad Zahoor Khan, Yadi Jing, Xiaotong Liu, Wenting Chen, Mingyang Geng, Bing Liu, Yunliang Zhang, Shishuai Xing, Yongdong Peng, Changfa wang   +10 more
doaj   +1 more source

The Donkey Genome: From Evolutionary Insights to Sustainable Breeding Strategies

Animals
Donkeys (Equus asinus) are economically and ecologically important livestock species whose genetic potential remains underexplored. This review synthesizes recent advances in donkey genomics, tracing their evolutionary history while evaluating current ...
Qifei Zhu, Muhammad Zahoor Khan, Yadi Jing, Mingyang Geng, Xuemin Zhang, Yunfan Zheng, Xianggang Cao, Yongdong Peng, Changfa Wang   +8 more
doaj   +1 more source

Multi‐Omics Integration Identifies a CDH3‐Associated Malignant Epithelial State and Immunosuppressive Niche to Predict Prognosis in Thymic Epithelial Tumors

Advanced Science, EarlyView.
Single‐cell, spatial, molecular, and pathology analyses identify a CDH3‐associated malignant epithelial state in thymic epithelial tumors. This state links stem‐like and EMT programs to M2 macrophage–rich immunosuppressive niches, genomic instability, poor survival, and drug vulnerability.
Yuntao Feng, Jingyu Chen, Lang Xia, Tao Wang, Yuzhou Wang, Lei Zhang, Guofang Zhao, Long Xu, Juemin Yu, Yunlang She, Junqi Wu, Yue Zhao, Chang Chen, Deping Zhao   +13 more
wiley   +1 more source

Deep Learning‐Driven Discovery and Engineering of an Efficient PETase for Depolymerization and Detoxification of PET Microplastics Under Physiological Conditions

Advanced Science, EarlyView.
A deep learning–driven pipeline mining 246 million protein sequences uncovers AhPETase, an evolutionarily distinct PET hydrolase. Engineered variant AhPETaseM1 degrades post‐consumer PET microplastics under physiological conditions and reverses microplasticinduced cytotoxicity in human lung and colon cells, establishing enzymatic microplastic ...
Yuxuan Wang, Shijie He, Yuheng Chang, Sheng Mao, Jianan Canal Li, Binbin Chen, Hongxun Gao, Mingchun Xu, Chenxu Liu, Yajie Wang   +9 more
wiley   +1 more source

Evaluation of Celastrol Antiviral Activity Against Equid Alphaherpesvirus Type 8 Infection

Viruses
Equid alphaherpesvirus type 8 (EHV-8) is a contagious pathogen that causes reproductive disorders, respiratory diseases, and viral encephalitis in equids, resulting in significant economic losses for the global horse and donkey industries.
Yue Yu, Jiayu Wang, Lian Ruan, Li Chen, Muhammad Zahoor Khan, Anrong You, Changfa Wang, Liangliang Li, Huiying Ren, Tongtong Wang, Wenhua Liu   +10 more
doaj   +1 more source

High Efficiency Ecological Concrete

Waste glass is cementitious in nature when it is finely ground, and especially when it is ground in a wet environment it can be finer than Portland cement.
Kara, Patricija, Korjakins, Aleksandrs
core  

Asymmetric Evolution of Antennal Cell Types Underlies a Derived Ammonia‐Sensing Logic for Ecological Adaptation in Bactrocera dorsalis

Advanced Science, EarlyView.
Comparative antennal single‐nucleus transcriptomics reveals cell‐type‐dependent transcriptomic divergence in Bactrocera dorsalis, with similar structural cells but divergent sensory neurons. This neuronal diversification is associated with a distinct ammonia‐sensing pathway, linking female attraction to bird‐dropping‐associated cues with nutritional ...
Wei Liu, Shuai Zhang, Jie Zhang, Zhen Tian, Yuanbo Xue, Ling Yang, Jun Xu, Guirong Wang   +7 more
wiley   +1 more source