Results 151 to 160 of about 165,447 (318)

Cryptic Kultarr: Integrative Taxonomy Reveals Unrecognized Species of Carnivorous Marsupial (Dasyuridae: Antechinomys) in Arid Australia

open access: yesEcology and Evolution, Volume 15, Issue 7, July 2025.
Conservation is impossible if we have not described all of the species we aim to conserve. This paper reviews the taxonomy of the kultarr (Antechinomys laniger) a small arid‐zone specialist native to Australia. We describe a new species, A. auritus sp. nov., and resurrect a formerly recognized species, A. spenceri. ABSTRACT Globally, mammal species are
Cameron S. Dodd   +5 more
wiley   +1 more source

The dual‐coding gene SLC35A4 protects against oxidative stress

open access: yesProtein Science, Volume 34, Issue 7, July 2025.
Abstract Alternative proteins (AltProts) represent a newly recognized class of biologically active proteins encoded from alternative open reading frames (AltORFs) within already annotated genes. This study focuses on the SLC35A4 gene, which encodes both the reference protein SLC35A4 and the alternative protein AltSLC35A4.
Ikram Ajala   +7 more
wiley   +1 more source

Scaling‐up RADseq methods for large datasets of non‐invasive samples: Lessons for library construction and data preprocessing

open access: yesMolecular Ecology Resources, Volume 25, Issue 5, July 2025.
Abstract Genetic non‐invasive sampling (gNIS) is a critical tool for population genetics studies, supporting conservation efforts while imposing minimal impacts on wildlife. However, gNIS often presents variable levels of DNA degradation and non‐endogenous contamination, which can incur considerable processing costs. Furthermore, the use of restriction‐
Larissa S. Arantes   +6 more
wiley   +1 more source

人 resistin 基因原核表达载体构建和表达

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2004
【目的】构建人resistin基因原核表达载体并观察其表达,为开展对resistin的研究打下实验基础。【方法】酚氯仿一步法从一位2型糖尿病合并胆管癌患者腹壁皮下脂肪组织抽取总RNA,RT-PCR法扩增出re-sistin基因cDNA,经测序后,PCR产物亚克隆入T载体,用EcoRI和KpnI分别酶切重组T质粒和原核表达载体pGENKS,然后resistin基因片段和表达载体通过T4DNA连接酶进行定向连接,用核酸内切酶EcoRI、KpnI酶切和测序方法鉴定出重组表达质粒 ...
doaj  

Evaluating restriction enzyme selection for reduced representation sequencing in conservation genomics

open access: yesMolecular Ecology Resources, Volume 25, Issue 5, July 2025.
Abstract Conservation genomic studies in non‐model organisms generally rely on reduced representation sequencing techniques based on restriction enzymes to identify population structure as well as candidate loci for local adaptation. While the expectation is that the reduced representation of the genome is randomly distributed, the proportion of the ...
Ainhoa López   +3 more
wiley   +1 more source

Optimizing ddRAD sequencing for population genomic studies with ddgRADer

open access: yesMolecular Ecology Resources, Volume 25, Issue 5, July 2025.
Abstract Double‐digest Restriction‐site Associated DNA sequencing (ddRADseq) is widely used to generate genomic data for non‐model organisms in evolutionary and ecological studies. Along with affordable paired‐end sequencing, this method makes population genomic analyses more accessible.
Aparna Lajmi, Felix Glinka, Eyal Privman
wiley   +1 more source

Stereoselective interaction with chiral phosphorothioates at the central DNA kink of the EcoRI endonuclease-GAATTC complex.

open access: hybrid, 1992
David Lesser   +5 more
openalex   +1 more source

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