Results 151 to 160 of about 223,024 (272)
MicrobiologyOpen, Volume 15, Issue 2, April 2026.Tn‐seq analysis of Thermus thermophilus genome reveals insertions in almost all genes, this is, there is no clear discrimination of essential genes. A similar result has not been reported in other Tn‐seq analysis of bacterial genomes so far. The polyploidy of the Thermus genome could explain the apparent non‐essentiality of key genes.
Cristina L. Gómez‐Campo +6 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】构建人resistin基因原核表达载体并观察其表达,为开展对resistin的研究打下实验基础。【方法】酚氯仿一步法从一位2型糖尿病合并胆管癌患者腹壁皮下脂肪组织抽取总RNA,RT-PCR法扩增出re-sistin基因cDNA,经测序后,PCR产物亚克隆入T载体,用EcoRI和KpnI分别酶切重组T质粒和原核表达载体pGENKS,然后resistin基因片段和表达载体通过T4DNA连接酶进行定向连接,用核酸内切酶EcoRI、KpnI酶切和测序方法鉴定出重组表达质粒 ...
doaj
Evolving resistance patterns in Tetranychus urticae and Bemisia tabaci in Greece
Pest Management Science, Volume 82, Issue 4, Page 3544-3551, April 2026.Greek populations of T. urticae and B. tabaci exhibit evolving multi‐resistance, confirmed by bioassays and molecular markers, highlighting the urgent need for evidence‐based resistance monitoring in IPM. Abstract BACKGROUND Pesticide resistance in agricultural pests remains a major barrier to effective and sustainable crop protection.
Anastasia Kampouraki +8 more
wiley +1 more source
Revista do Instituto de Medicina Tropical de São Paulo, 1997 Forty isolates of adenovirus type 7 were analized by restriction enzyme digestion with BamHI, SmaI, EcoRI and HindIII. These isolates were obtained from acute respiratory disease patients during the years 1980 to 1991.
MORAES Marcia T.B. +5 more
doaj
A New Breeding Technique for F1 Hybrid Production From Self‐Incompatible Species
Plant Biotechnology Journal, EarlyView.
Rowan P. Herridge +4 more
wiley +1 more source
Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways
Microbial Biotechnology, Volume 19, Issue 4, April 2026.Engineered Saccharomyces cerevisiae reveals how cell wall remodelling and membrane trafficking coordinate extracellular vesicle biogenesis. Interferon overexpression and chs3 deletion enhance EV secretion and reshape SNARE‐, Rab‐ and ESCRT‐associated pathways, establishing yeast as a versatile platform for EV engineering and therapeutic cargo loading ...
Yueyan Li +11 more
wiley +1 more source
The essential role of 2,4-dienoyl-CoA reductase for the degradation of complex fatty acid mixtures. [PDF]
mBioSchiaffi V +5 more
europepmc +1 more source
Microbial Biotechnology, Volume 19, Issue 4, April 2026.This study demonstrated that SACE_Lrp binds to the ilvE promoter in a BCAA‐dependent manner, and BCAAs addition results in a higher increase of ilvE transcription and erythromycin production in mutant ΔSACE_Lrp than in the original strain S. erythraea A226.
Xinlu Cai +9 more
wiley +1 more source
Short-Term Epigenetic Responses of <i>Pinus brutia</i> to Fire Stress: Insights from a Prescribed Burning in Greece. [PDF]
Genes (Basel)Avramidou EV +3 more
europepmc +1 more source
SanA Plays a Role in Peptidoglycan Integrity in Escherichia coli
Molecular Microbiology, Volume 125, Issue 4, Page 343-357, April 2026.SanA is a novel regulator of peptidoglycan synthesis, potentially through functional interplay with PBP1B‐dependent pathways. ABSTRACT Peptidoglycan synthesis and degradation are both essential for bacterial growth, and damaged peptidoglycan must be continuously repaired. In Escherichia coli, peptidoglycan required for cell elongation is synthesized by
Honoka Yamaguchi +6 more
wiley +1 more source