Results 121 to 130 of about 1,358 (148)
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The modulation of ecto-nucleotidase activities by glutamate in cultured cerebellar granule cells
NeuroReport, 2000Several enzymes hydrolyze ATP, producing ADP which is hydrolyzed to AMP. Ecto-5'-nucleotidase produces adenosine from AMP. Glutamate (Glu) is an excitatory neurotransmitter and increases extracellular adenosine levels, which is considered an important inhibitory neuromodulator. Here we show that Glu activates ADP and AMP hydrolysis.
C R, Boeck +4 more
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BIOCHEMISTRY, LOCALIZATION AND FUNCTIONAL ROLES OF ECTO-NUCLEOTIDASES IN THE NERVOUS SYSTEM
Progress in Neurobiology, 1996Nucleotides such as ATP, ADP, UTP or the diadenosine polyphosphates and possibly even NAD+ are extracellular signaling substances in the brain and in other tissues. Enzymes located on the cell surface catalyze the hydrolysis of these compounds and thus limit their spatio-temporal activity.
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Extracellular nucleotides regulate the expression and activity of ecto-nucleotidases by osteoblasts
Bone, 2010Previous work has shown that extracellular nucleotides (>1 μM ATP/UTP) prevent bone nodule formation in vitro by blocking mineralisation of the collagenous matrix. The ratio of phosphate (Pi) to pyrophosphate (PPi) in the bone microenvironment is a fundamental regulator of mineralisation.
Isabel Orriss, Timothy Arnett
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Habituation to an open field alters ecto-nucleotidase activities in rat hippocampal synaptosomes
Neuroscience Letters, 2007ATP and adenosine may play a role in the mechanisms of synaptic plasticity and memory formation. Previous studies have shown that ecto-nucleotidase activities are altered during memory consolidation of an aversive task named step-down inhibitory avoidance. Here we investigate ecto-nucleotidase activities in hippocampal synaptosomes of rats submitted to
Eduardo Luiz, Pedrazza +4 more
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Reactive red 2: a P2y-selective purinoceptor antagonist and an inhibitor of ecto-nucleotidase
Naunyn-Schmiedeberg's Archives of Pharmacology, 1995Effects of reactive red 2 and its parent compound acid red 33 were studied in rat vas deferens and guinea-pig taenia coli. In rat vas deferens, reactive red 2(1 to 10 microM) shifted the concentration-response curve for the P2X-purinoceptor-mediated contraction effect of alpha, beta-methylene ATP slightly to the right and progressively decreased the ...
R, Bültmann, K, Starke
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Ecto-nucleotidase activity in pancreatic islet cells
2007Comm. 43rd Annual Meeting of the European Association for the Study of Diabetes – Amsterdam (The Netherlands), 17.09 ...
Giroix, Marie-Hélène +8 more
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Thyroid hormone stimulates 5′-ecto-nucleotidase of neonatal rat ventricular myocytes
Molecular and Cellular Biochemistry, 2004Degradation of adenine nucleotides in myocardial cells has important physiological implications associated with the regulation of the high-energy phosphate precursor pool and the production of adenosine. Adenosine may be released as from cells or, following adenine nucleotides release, they may be metabolized and rapidly converted to adenosine via the ...
Marcela Sorelli, Carneiro-Ramos +5 more
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Microcalorymetric measurement of ecto-nucleotidase activity of endothelial cells
1999Heat flux (HF) reflecting the metabolic activity of the perfused pig aortic endothelial cells (PAEC) grown on microcarrier beads was measured microcalorimetrically (LKB thermal monitor) and found to be 231.0 ą65.5 ěW/mg cell protein or 46.34 ą 8.48 pW per single cell.
Čulić, Ognjen +3 more
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Roles of Asp54 and Asp213 in Ca2+ utilization by soluble human CD39/ecto-nucleotidase
Archives of Biochemistry and Biophysics, 2002Soluble human CD39 (solCD39) rapidly metabolizes nucleotides, especially ADP released from activated platelets, thereby inhibiting further platelet activation and recruitment. Using alanine substitution mutagenesis, we established a functional role for aspartates D54 and D213 in solCD39.
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Ecto-nucleotidases terminate purinergic signalling in the cochlear endolymphatic compartment.
Neuroreport, 1998There is strong evidence for a purinergic signalling system in the inner ear which regulates auditory sensitivity. This study describes the terminating mechanism for purinergic signalling in the cochlear endolymphatic compartment via ecto-nucleotidases. Exogenous ATP was introduced into the scala media (SM) of the isolated, perfused guinea-pig cochlea,
S M, Vlajkovic +4 more
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