Results 81 to 90 of about 5,372,457 (283)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Finite Element Analysis of Dynamic Meshing Characteristic of Skew Bevel Gear with Equivocal Modification

open access: yesJixie chuandong, 2022
On the basis of the principal of gear generating machining method,the gear flank mathematical model and gear tooth flank contact line equation of skew bevel gears with equivocal modification are derived,the contact finite element dynamic analysis model ...
Xiaoping Ran, Liyao Gu, Jianfan Wang
doaj  

An explicit solution to a three-dimensional wedge problem considering two edges effect

open access: yesFriction, 2019
The paper presents an explicit matrix algorithm to solve the problem of an elastic wedge with three loaded surfaces. The algorithm makes use of a recently published concept of transformation matrix, by which the original surface loads are converted to ...
L. Guo   +4 more
doaj   +1 more source

Classical diffusion of N interacting particles in one dimension: General results and asymptotic laws

open access: yes, 1999
I consider the coupled one-dimensional diffusion of a cluster of N classical particles with contact repulsion. General expressions are given for the probability distributions, allowing to obtain the transport coefficients.
Bouchaud J.-Ph.   +5 more
core   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

open access: yesFEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui   +10 more
wiley   +1 more source

The effects of disordered edge and vanishing friction in microscale structural superlubric graphite contact

open access: yesNature Communications
Structural superlubricity (SSL), a state of ultralow friction and no wear between two solid surfaces in contact, offers a fundamental solution for reducing friction and wear. Recent studies find that the edge pinning of SSL contact dominates the friction.
Hengqian Hu   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

Effect of Contact Geometry on the Contact Stresses in a Flat with Round Edge Contact

open access: yesFracture and Structural Integrity, 2019
The blade-disc dovetail interface in an aero-engine compressor is characterized by a non-uniform pressure distribution which can be obtained by an equivalent flat with round edge-on-plate configuration.
Pankaj Dhaka, Raghu Vasu Prakash
doaj  

Nonequilibrium noise correlations in a point contact of helical edge states

open access: yes, 2013
We investigate theoretically the nonequilibrium finite-frequency current noise in a four-terminal quantum point contact of interacting helical edge states at a finite bias voltage.
A. O. Gogolin   +6 more
core   +1 more source

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