Results 21 to 30 of about 1,204,990 (209)

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

Molecular Ecology, 2022
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and numerous studies have investigated the consistency of this technique with traditional morpho‐taxonomic approaches.
F. Keck, Rosetta C. Blackman, Raphael Bossart, Jeanine Brantschen, Marjorie Couton, Samuel Hürlemann, D. Kirschner, Nadine Locher, Heng Zhang, F. Altermatt   +9 more
semanticscholar   +1 more source

Comparative environmental RNA and DNA metabarcoding analysis of river algae and arthropods for ecological surveys and water quality assessment

Scientific Reports, 2022
Environmental DNA (eDNA) metabarcoding is widely used for species analysis, while the use of environmental RNA (eRNA) metabarcoding is more limited. We conducted comparative eDNA/eRNA metabarcoding of the algae and arthropods (aquatic insects) in water ...
Kaede Miyata, Yasuaki Inoue, Yuto Amano, Tohru Nishioka, Tomohisa Nagaike, Takamitsu Kawaguchi, Osamu Morita, Masayuki Yamane, Hiroshi Honda   +8 more
doaj   +1 more source

eDNA metabarcoding of log hollow sediments and soils highlights the importance of substrate type, frequency of sampling and animal size, for vertebrate species detection

Environmental DNA, 2022
Fauna monitoring often relies on visual monitoring techniques such as camera trapping, which have biases leading to underestimates of vertebrate species diversity.
Ethan Ryan, Philip Bateman, Kristen Fernandes, Mieke van derHeyde, Paul Nevill   +4 more
doaj   +1 more source

Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna [PDF]

Molecular Ecology Resources.
ABSTRACT Subterranean ecosystems host a diverse range of ancient fauna, but studying these ecosystems is challenging due to significant sampling difficulties. Environmental DNA (eDNA) metabarcoding offers a promising approach for monitoring subterranean biodiversity, yet issues such as primer bias and non‐target amplification can complicate its ...
van der Heyde M, Curran M, Floeckner S, Nevill P, White N, Austin A, Guzik M.   +6 more
europepmc   +2 more sources

Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global eDNA

Global Ecology and Conservation, 2019
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity wherein samples are taken from the environment via water, sediment or air from which DNA is extracted, and then amplified using general or universal primers in polymerase ...
Krista M. Ruppert, Richard J. Kline, Md Saydur Rahman   +2 more
semanticscholar   +1 more source

Calibrating Environmental DNA Metabarcoding to Conventional Surveys for Measuring Fish Species Richness

Frontiers in Ecology and Evolution, 2020
The ability to properly identify species present in a landscape is foundational to ecology and essential for natural resource management and conservation.
Mary E. McElroy, Terra L. Dressler, Georgia C. Titcomb, Emily A. Wilson, Kristy Deiner, Tom L. Dudley, Erika J. Eliason, Nathan T. Evans, Steven D. Gaines, Kevin D. Lafferty, Kevin D. Lafferty, Gary A. Lamberti, Yiyuan Li, David M. Lodge, Milton S. Love, Andrew R. Mahon, Michael E. Pfrender, Mark A. Renshaw, Kimberly A. Selkoe, Christopher L. Jerde   +19 more
doaj   +1 more source

Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient [PDF]

Metabarcoding and Metagenomics, 2018
Monitoring aquatic biodiversity through DNA extracted from environmental samples (eDNA) combined with high-throughput sequencing, commonly referred to as eDNA metabarcoding, is increasing in popularity within the scientific community.
Jonas Bylemans, Dianne M. Gleeson, Mark Lintermans, Christopher M. Hardy, Matthew Beitzel, Dean M. Gilligan, Elise M. Furlan   +6 more
doaj   +2 more sources

Environmental DNA metabarcoding for fish community analysis in backwater lakes: A comparison of capture methods.

PLoS ONE, 2019
The use of environmental DNA (eDNA) methods for community analysis has recently been developed. High-throughput parallel DNA sequencing (HTS), called eDNA metabarcoding, has been increasingly used in eDNA studies to examine multiple species.
Kazuya Fujii, Hideyuki Doi, Shunsuke Matsuoka, Mariko Nagano, Hirotoshi Sato, Hiroki Yamanaka   +5 more
doaj   +1 more source

An efficient early‐pooling protocol for environmental DNA metabarcoding

Environmental DNA, 2022
Environmental DNA (eDNA) metabarcoding, a method that applies high‐throughput sequencing and universal primer sets to eDNA analysis, has been a promising approach for efficient, comprehensive biodiversity monitoring.
Masayuki Ushio, Saori Furukawa, Hiroaki Murakami, Reiji Masuda, Atsushi J. Nagano   +4 more
doaj   +1 more source

Quantitative environmental DNA metabarcoding shows high potential as a novel approach to quantitatively assess fish community

Scientific Reports, 2022
The simultaneous conservation of species richness and evenness is important to effectively reduce biodiversity loss and keep ecosystem health. Environmental DNA (eDNA) metabarcoding has been used as a powerful tool for identifying community composition ...
Satsuki Tsuji, Ryutei Inui, Ryohei Nakao, Seiji Miyazono, Minoru Saito, Takanori Kono, Yoshihisa Akamatsu   +6 more
doaj   +1 more source