Results 101 to 110 of about 2,174,249 (299)

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Genomic analysis of Salmonella isolated from surface water and animal sources in Chile reveals new T6SS effector protein candidates

Frontiers in Microbiology
Type VI Secretion Systems (T6SS), widely distributed in Gram-negative bacteria, contribute to interbacterial competition and pathogenesis through the translocation of effector proteins to target cells.
Fernando A. Amaya, Carlos J. Blondel, Felipe Reyes-Méndez, Dácil Rivera, Andrea Moreno-Switt, Magaly Toro, Magaly Toro, Consuelo Badilla, Carlos A. Santiviago, David Pezoa, David Pezoa   +10 more
doaj   +1 more source

Divergence of Legionella Effectors Reversing Conventional and Unconventional Ubiquitination

Frontiers in Cellular and Infection Microbiology, 2020
The intracellular bacterial pathogen Legionella pneumophila employs bacteria-derived effector proteins in a variety of functions to exploit host cellular systems.
Tomoe Kitao, Hiroki Nagai, Hiroki Nagai, Tomoko Kubori, Tomoko Kubori   +4 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Effector-Binding-Directed Dimerization and Dynamic Communication between Allosteric Sites of Ribonucleotide Reductase

, 2019
Proteins forming dimers or larger complexes can be strongly influenced by their effector-binding status. We investigated how the effector-binding event is coupled with interface formation via computer simulations, and we quantified the correlation of two
Tongye Shen (203925), Richard J. Lindsay (1649947), Bill Pham (6181139)   +2 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Lines of evidence that have been used for predicting fungal effector proteins and examples for fungal effector prediction pipelines.

, 2015
Lines of evidence that have been used for predicting fungal effector proteins and examples for fungal effector prediction pipelines.
Jennifer M. Taylor (29844), Donald M. Gardiner (134515), Jana Sperschneider (561354), Peter N. Dodds (116560), Karam B. Singh (228088), John M. Manners (134529)   +5 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Analysis of Rho GTPase signalling pathways regulating epithelial morphogenesis [PDF]

, 2010
Rho GTPases are molecular switches controlling many aspects of cell behaviour, including cell cycle progression, cell division, migration and morphology. The activity of Rho proteins is regulated by conformational changes induced by binding of guanine
Wallace, S.
core