Results 1 to 10 of about 1,204,816 (197)

Release of protein N-glycans by effectors of a Hofmann carboxamide rearrangement

open access: yesFrontiers in Molecular Biosciences, 2022
Background: Chemical methods for glycan release have gained traction because of their cost efficiency, accelerated reaction time and ability to release glycans not amenable to enzymatic cleavage.
Mumtaz Kasim   +5 more
doaj   +1 more source

Seamless Coupling of Chemical Glycan Release and Labeling for an Accelerated Protein N-Glycan Sample Preparation Workflow

open access: yesBioengineering, 2023
Analytical methods fr direct quantitative N-glycan analysis require a sequence of sample preparation and clean-up steps that result in reduced glycan recovery. Therefore, we aimed to combine glycan release and labeling steps. Based on the hypothesis that
Mumtaz Kasim   +8 more
doaj   +1 more source

Reporting two hemoglobin J Iran cases, molecular follow-up, or is it insignificant? [PDF]

open access: yesCaspian Journal of Internal Medicine, 2023
Background: Hemoglobin J is one of the fast hemoglobin that has a more negative charge due to β77His→Asp substitution. Acquisition of this hemoglobin is not associated with any specific clinical sign, but the combination of this hemoglobinopathy with ...
Amin Solouki   +3 more
doaj  

Comparing protocols of DNA extraction from Escherichia coli: Analysis of purity and concentration by gel electrophoresis

open access: yesBaghdad Journal of Biochemistry and Applied Biological Sciences, 2022
Background and objectives: Given the difficulty in establishing an ideal method that can successfully extract bacterial deoxyribonucleic acid (DNA) in Gram-negative bacteria, the objective of this work was to compare protocols for the extraction of ...
Oslaene Alves de Brito   +8 more
doaj   +1 more source

High resolution two-dimensional electrophoresis of proteins.

open access: yesJournal of Biological Chemistry, 1975
A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Due to its resolution and sensitivity, this technique is a powerful tool for the analysis and detection of proteins from complex ...
P. O’Farrell
semanticscholar   +1 more source

Bisalbuminemia in a Hypothyroid Patient with Diabetes: A Case Report [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2015
Bisalbuminemia is a rarely encountered anomaly characterized by presence of bifid albumin bands or a single widened albumin band in electrophoretogram. Inherited bisalbuminemia is quite rare and inherited as an autosomal dominant form. The acquired form
Jeevan K. Shetty   +3 more
doaj   +1 more source

Molecular genetic techniques in current biomedical research. Part I: Theoretical basis of PCR -diagnostics

open access: yesФундаментальная и клиническая медицина, 2020
Molecular genetic techniques represent an essential part of current biomedical research. Among them, polymerase chain reaction (PCR) is the most widespread. This approach reproduces the natural mechanism of nucleic acid replication in vitro, specifically
A. N. Volkov, L. V Nacheva
doaj   +1 more source

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

open access: yesJournal of Biological Chemistry, 1969
Forty proteins with polypeptide chains of well characterized molecular weights have been studied by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate following the procedure of Shapiro, Vinuela, and Maizel (Biochem.
K. Weber, M. Osborn
semanticscholar   +1 more source

The Evolution of the Sickle Cell Anaemia in the Region of Kinshasa: a Mathematic Modelling

open access: yesHipotenusa, 2022
We attempt to study the evolution of the sickle cell anaemia in the region of Kinshasa by proposing three models based on Markov processes.  The first two models namely, the idealistic and the quasi- idealistic models fail to completely describe the data
R.Gilles Bokolo   +3 more
doaj   +1 more source

Identification of short-stem gene Rht-B1 in the winter soft wheat samples

open access: yesЗерновое хозяйство России, 2020
The current paper has presented the estimation results of the collection material of winter soft wheat according to the presence of alleles of short-stem gene Rht-B1.
N. T. Kupreyshvily   +3 more
doaj   +1 more source

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