Results 1 to 10 of about 560,319 (158)
Electrophoresis of positioned nucleosomes [PDF]
We present in this paper an original approach to compute the electrophoretic mobility of rigid nucleo-protein complexes like nucleosomes. This model allows to address theoretically the influence of complex position along DNA, as well as wrapped length of DNA on the electrophoretic mobility of the complex. The predictions of the model are in qualitative
Allison+35 more
arxiv +12 more sources
The repton model of gel electrophoresis [PDF]
We discuss the repton model of agarose gel electrophoresis of DNA. We review previous results, both analytic and numerical, as well as presenting a new numerical algorithm for the efficient simulation of the model, and suggesting a new approach to the model's analytic solution.
Barkema+17 more
arxiv +3 more sources
Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with different proteins, while most protein chemistry methods work mainly, if not only, in water-based ...
Adessi+103 more
arxiv +6 more sources
Magnetophoresis of Tagged Polymers [PDF]
We present quantitative results for the drift velocity of a polymer in a gel if a force (e.g. through an electric or magnetic field) acts on a tag, attached to one of its ends. This is done by introducing a modification of the Rubinstein-Duke model for electrophoresis of DNA.
Barkema, G. T., Schütz, G. M.
arxiv +4 more sources
Electrophoresis of pepsin [PDF]
Arne Tiselius+2 more
openalex +4 more sources
Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő+3 more
core +1 more source
Microfluidic free-flow electrophoresis for proteomics-on-a-chip [PDF]
A new free-flow electrophoresis microchip with integrated permeable membranes was developed, and different substances were separated by free-flow zone electrophoresis, free-flow isoelectric focusing and free-flow field step electrophoresis.
Kohlheyer, Dietrich
core +8 more sources
Microchip electrophoresis bioanalytical applications [PDF]
Microchip electrophoresis (MCE) is a novel analytical technique resulting from miniaturization of capillary electrophoresis (CE) to a planar microfabricated separation device.
Vlcková, Markéta
core +1 more source
Variations on a theme: Changes to electrophoretic separations that can make a difference [PDF]
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
arxiv +1 more source
Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]
Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv +1 more source