Results 1 to 10 of about 357,883 (98)
Proceedings of the National Academy of Sciences of the United States of America, 1989 We developed mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms. This method follows digestion of genomic DNA with restriction endonucleases, denaturation in alkaline solution, and ...
Masato Orita +4 more
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Definitions, 2020 Gel electrophoresis is a method of separating large molecules (such as DNA fragments or proteins) from a mixture of similar molecules by electrophoretic filtration through a gelatinous matrix.
Phil Vercoe, Agarose Gel Well
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Electrophoresis, 2018 Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables ...
Alexander Stolz +5 more
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Cold Spring Harbor Protocols, 2019 This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
Michael R Green, J. Sambrook
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Capillary Electrophoresis: Trends and Recent Advances
Analytical Chemistry, 2018 Robert L. C. Voeten,†,‡ Iro K. Ventouri,‡,§ Rob Haselberg,*,† and Govert W. Somsen† †Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, The ...
Robert L C Voeten +3 more
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Electrophoresis, 2018 One of the most cited limitations of capillary and microchip electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of online/in ...
M. Breadmore +15 more
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Capillary Electrophoresis Separations of Glycans
Chemical Reviews, 2018 Capillary electrophoresis has emerged as a powerful approach for carbohydrate analyses since 2014. The method provides high resolution capable of separating carbohydrates by charge-to-size ratio.
Grace Lu +4 more
semanticscholar +1 more source
Nucleic Acids Research, 1981 We describe the use of gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions. The method, which we call protein distribution analysis, is simple, sensitive and yields thermodynamically rigorous
M. Fried, D. Crothers
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Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.
Proceedings of the National Academy of Sciences of the United States of America, 2000 Proteome analysis is most commonly accomplished by a combination of two-dimensional gel electrophoresis (2DE) to separate and visualize proteins and mass spectrometry (MS) for protein identification.
S. Gygi +4 more
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Nucleic Acids Res., 1981 The use of gel electrophoresis for quantitative studies of DNA-protein interactions is described. This rapid and simple technique involves separation of free DNA from DNA-protein complexes based on differences in their electrophoretic mobilities in ...
M. Garner, A. Revzin
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