Results 131 to 140 of about 1,487,249 (257)

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Increased Insulin Action, Glucose Metabolism and Muscle Function in Supervillin‐Knockout and Supervillin‐Mutant Mice

Cytoskeleton, EarlyView.
ABSTRACT We here describe mouse models with complementary homozygous Svil mutations. In skeletal muscle, Svil‐Mut mice express the Svil‐encoded N‐terminus fused to the βgal‐neo gene‐trap tag and lack the highly conserved archvillin C‐terminus; Svil‐KO mice lack expression of all known Svil‐encoded proteins; and Svil‐LoxP mice contain loxP sites for ...
Tara C. Smith, Lauren A. Tauer, Randall H. Friedline, Xiaodi Hu, Duy Tran, Yangyi E. Luo, Nacima Hadjout‐Rabi, Elisabeth R. Barton, Jason K. Kim, Elizabeth J. Luna   +9 more
wiley   +1 more source

Comparison of gel and capillary electrophoresis of proteins

, 2012
Electrophoresis of serum proteins is one of the methods used in laboratory diagnosis of pathological conditions, but also for research purposes. This is a one-step method, which involves isolation of individual proteins from a mixture (mostly blood ...
Krbec, Michal
core  

Difference gel electrophoresis.

, 2018
Difference gel electrophoresis (DIGE) was invented to circumvent the inherent variability of 2-DE. This variability is a natural consequence of separating thousands of proteins over a large space, such as a 15 x 20 cm slab of polyacrylamide gel.
Kai Stühler (5063747), Jonathan Minden (3890494), Susan R. Dowd (5063108), Helmut E. Meyer (138091)   +3 more
core   +1 more source

Selective Modification of the Product Profile of Biocatalytic Hydrolyzed PET via Product‐Specific Medium Engineering

ChemSusChem, Volume 18, Issue 6, March 15, 2025.
The product profile of enzymatically hydrolyzed PET can be modified by medium engineering and thereby adapted to a desired product. TPA, MHET or BHET can be forced as the predominant product using a basic pH (blue), 25 % ethylene glycol (EG) and IsPETasewt (green) or ≥25 % EG and LCCICCG (pink), respectively.
Tobias Heinks, Katrin Hofmann, Simon Last, Igor Gamm, Luise Blach, Ren Wei, Uwe T. Bornscheuer, Christof Hamel, Jan von Langermann   +8 more
wiley   +1 more source

Purification of crude cellulase obtained from Aspergillus foetidus and its immobilization in agar xerogel: A comparative study with commercial cellulase immobilized in similar support

Food Biomacromolecules, EarlyView.
Agar xerogel was used to immobilize cellulase. Properties of partially purified cellulase from Aspergillus foetidus and commercial cellulase were compared after immobilization. Abstract This research study is a comparative study of the characteristics of partially purified cellulase produced from Aspergillus foetidus through solid‐state fermentation ...
Bhaskar Jyoti Kalita, Nandan Sit
wiley   +1 more source

Conducting Polymer Electrodes for Gel Electrophoresis

, 2013
In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that p-conjugated polymers such as poly(3,4-
Bengtsson, Katarina,, Katarina Bengtsson, Robinson, Nathaniel D,, Bengtsson, Katarina, Sara Nilsson (524287), Robinson, Nathaniel D, Nilsson, Sara, Sara Nilsson, Nathaniel D. Robinson, Nathaniel D. Robinson (524288), Katarina Bengtsson (524286), Nathaniel D Robinson, Nilsson, Sara,, Bengtsson Katarina, Nilsson Sara, Robinson Nathaniel D.   +15 more
core   +1 more source

Chemistry Underlying the Behavior of Food and Agricultural Components During Industrial Manufacturing Processes and Its Implications in Quality Assurance, Food Quality, and Food Safety Management: A Systematic Review

Food Safety and Health, EarlyView.
Industrial manufacturing processes have transformative effects on the components of food matrix. These transformations in food matrix and components have direct implications for quality assurance (QA), food quality (e.g., nutritional profile and texture), and food safety management (e.g., pathogen control and toxin mitigation). ABSTRACT This systematic
Chinaza Godswill Awuchi
wiley   +1 more source

Research on seaweeds and mollusks [PDF]

, 1995
Research on seaweeds focused on the carrageenan-producing Kappaphycus alvarezii and the agar-producing Gracilaria spp. Growth of K. alvarezii was better on horizontal lines than on vertical or cluster lines from bamboo rafts. All morphotypes (brown green,
Hurtado-Ponce, Anicia Q.
core  

Lactobacillus derived small RNAs protect against Klebsiella pneumoniae infection

iMeta, EarlyView.
This study reveals that plant‐derived natural products confer protection against carbapenem‐resistant Klebsiella pneumoniae (CRKP) infection via remodeling the gut microbiota, with Lactobacillus identified as the core beneficial genus. Mechanistically, Lactobacillus murinus (L.
Tingting Xu, Xiuying Tao, Dan Fang, Haijie Zhang, Zhiqiang Wang, Yuan Liu   +5 more
wiley   +1 more source