Results 31 to 40 of about 1,417,832 (307)

Activity staining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis [PDF]

, 2005
A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were ...
Gohel, Vipul, Chhatpar, HS, Vyas, Pranav
core   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Understanding the molecular epidemiology of the footrot pathogen Dichelobacter nodosus to support control and eradication programs [PDF]

, 2010
The Gram-negative anaerobe Dichelobacter nodosus is the primary etiologic agent of ovine footrot. Few studies of the genetic diversity and epidemiology of D. nodosus have been done, despite the economic cost and welfare implications of the disease.
Pitman, D., D. J. Hampson, Palmer, M., Hampson, D.J., N. B. Buller, Ashley, P., D. Pitman, R. B. Richards, Buller, N. B., Richards, R. B., P. Ashley, M. Palmer   +11 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2). [PDF]

, 2009
BACKGROUND: Genotyping of Plasmodium falciparum based on PCR amplification of the polymorphic genes encoding the merozoite surface proteins 1 and 2 (msp1 and msp2) is well established in the field of malaria research to determine the number and types of ...
Färnert, Anna, Kweku Margaret, Mårtensson, Andreas, Margaret Kweku, Mårtensson, A., Felger Ingrid, Felger, Ingrid, Mårtensson Andreas, Kweku, M., Liljander Anne, Ingrid Felger, Falk, Nicole, Farnert, A., Kweku, Margaret, Lisa Wiklund, Andreas Mårtensson, Felger, I., Anna Färnert, Liljander, A., Wiklund Lisa, Liljander, Anne, Falk Nicole, Anne Liljander, Wiklund, L., Falk, N., Färnert Anna, Wiklund, Lisa, Nicole Falk   +27 more
core   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Prevalência de hemoglobinas anormais em recém-nascidos da cidade de Natal, Rio Grande do Norte, Brasil Prevalence of abnormal hemoglobins in newborns in Natal, Rio Grande do Norte, Brazil

Cadernos de Saúde Pública, 2004
As hemoglobinopatias estão incluídas dentre as doenças hereditárias mais freqüentes nas populações humanas. Estudos realizados em diferentes regiões do Brasil têm demonstrado que as hemoglobinas anormais S e C são as mais prevalentes.
Maria Cristina Pignataro Emerenciano de Araújo, Édvis Santos Soares Serafim, Wivel Antonio Pereira de Castro Jr., Tereza Maria Dantas de Medeiros   +3 more
doaj   +1 more source

Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat [PDF]

, 2010
Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough.
Branlard, Gerard, Rogers, William John, Liu, Li, Ikeda, Tatsuya M, Wang Aili, Yoshida, H., Branlard, G., Xia, X., Kolman, Maía de Los Angeles, Roberto J Peña, Ma, Wujun, Ma, W., Rogers, William J, Wang, A., Lerner, S.E., Gerard Branlard, Zhonghu He, Lerner, Silvia E., He, Zhonghu, Yan, Y., Wang Linhai, Linhai Wang, Lerner Silvia E, Wang, Aili, He, Z., Silvia E Lerner, Rogers William J, Wujun Ma, Branlard, Gerard,, Peña, R., Kolman María A, Xia Xianchun, William J Rogers, Appels, R., Kolman, María A, Yoshida Hisashi, Kolman, M.A., Peña, Roberto J, Yoshida, Hisashi, Rogers, W.J., Liu, L., Lerner, Silvia E, Tatsuya M Ikeda, Ma Wujun, Branlard, Gerard, G., Wang, L., Yan, Yueming, Wang, Linhai, Appels, Rudi, Kolman, Maria de Los Angeles, Hisashi Yoshida, Appels Rudi, He Zhonghu, Peña Roberto J, Liu Li, Aili Wang, Ikeda Tatsuya M, María A Kolman, Peña, R.J., Li Liu, Yan Yueming, Xia, Xianchun, Ikeda, T.M., Yueming Yan, Rogers, John William, Xianchun Xia, Branlard Gerard, Rudi Appels, Ikeda, Tatsuya M., Peña, Roberto J.   +69 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Detection of mecA Gene in the Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Sokoto Journal of Medical Laboratory Science, 2021
Resistance coding in Staphylococcus aureus could be phenotypic or genotypic. In genotypic coding, mecA gene codes for the production of the altered penicillin-binding protein responsible for methicillin resistance.
Raji, M.I.O.* , Haido, A.U. , Garba, I. and Shuaibu, A.B.
doaj