Results 21 to 30 of about 223,709 (309)

Burkholderia cepacia complex bacteremia outbreaks among non-cystic fibrosis patients in the pediatric unit of a university hospital

open access: yesThe Turkish Journal of Pediatrics, 2021
Background. Burkholderia cepacia complex (Bcc) comprises multi-drug resistant, Gram-negative, motile, and aerobic bacteria. Bcc causes severe nosocomial infections particularly in patients with intravascular catheters and in those with cystic ...
Sinan Tüfekci   +4 more
doaj   +1 more source

A Method To Prepare An Economic Substitute For Agarose Gel Along With A Low-Cost Electrolyte For Functional DNA Gel Electrophoresis

open access: yesSyrian Journal for Science and Innovation
The use of agarose in electrophoresis is indispensable in modern biotechnology research. However, agarose is relatively expensive and not available in limited-budget scientific institutions, labs and educational facilities. Therefore, the search for more
Kamar Shayah   +4 more
doaj   +1 more source

Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae.

open access: yesPLoS ONE, 2023
Although many protocols have been previously developed for genomic DNA (gDNA) extraction from S. cerevisiae, to take advantage of recent advances in laboratory automation and DNA-barcode sequencing, there is a need for automated methods that can provide ...
Nina Alperovich   +2 more
doaj   +1 more source

Evidence that the 36kb plasmid of Brachyspira hyodysenteriae contributes to virulence [PDF]

open access: yes, 2011
Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Recently the genome of virulent Australian B. hyodysenteriae strain WA1 was sequenced, and a 36. kilobase (kb)
Bellgard, M.I.   +5 more
core   +2 more sources

Evaluation of a Multiplex PCR Assay for the Identification of Campylobacter jejuni and Campylobacter coli [PDF]

open access: yes, 2017
Background: Campylobacter jejuni and Campylobacter coli are identified as the major causes of acute gastroenteritis in humans. Because of the fastidious nature of Campylobacters, many clinical laboratories fail to routinely culture them.
Ghorbanalizadgan, Mehdi   +3 more
core   +1 more source

Electrophoretic separation of proteins [PDF]

open access: yes, 1981
There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins.
Ravindranath, M H, Subhashini, M H
core  

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

open access: yesFEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca   +6 more
wiley   +1 more source

Prevalência de hemoglobinas anormais em recém-nascidos da cidade de Natal, Rio Grande do Norte, Brasil Prevalence of abnormal hemoglobins in newborns in Natal, Rio Grande do Norte, Brazil

open access: yesCadernos de Saúde Pública, 2004
As hemoglobinopatias estão incluídas dentre as doenças hereditárias mais freqüentes nas populações humanas. Estudos realizados em diferentes regiões do Brasil têm demonstrado que as hemoglobinas anormais S e C são as mais prevalentes.
Maria Cristina Pignataro Emerenciano de Araújo   +3 more
doaj   +1 more source

Manual for starch gel electrophoresis: A method for the detection of genetic variation [PDF]

open access: yes, 1987
The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts ...
Aebersold, Paul B.   +4 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

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