Results 1 to 10 of about 705,442 (212)

A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting. [PDF]

open access: yesACS Omega
Birtwistle MR   +7 more
europepmc   +2 more sources

Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]

open access: yesPesquisa Veterinária Brasileira, 2021
: Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo   +4 more
doaj   +1 more source

The guideline for western blotting assay

open access: yesFood Frontiers, 2022
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang   +5 more
doaj   +1 more source

A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels

open access: yesBioTechniques, 2023
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment.
Kristan M Gomez   +5 more
doaj   +1 more source

Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.

open access: yesAl-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis   (
Ammar Q. Muhammed   +2 more
doaj   +1 more source

Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling [PDF]

open access: yesArchives of Biological Sciences, 2011
An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange ...
Asaduzzaman A.K.M.   +2 more
doaj   +1 more source

Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins

open access: yesBioTechniques, 1998
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj   +1 more source

SDS Agarose Gels for Analysis of Proteins

open access: yesBioTechniques, 1998
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel strength ...
Minjie Wu, Noriko Kusukawa
doaj   +1 more source

Polyacrylamide gel electrophoresis of soybean seed proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj   +1 more source

Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

open access: yesJournal of Pharmacopuncture, 2006
Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi   +2 more
doaj   +1 more source

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