Denaturing Polyacrylamide Gel Electrophoresis [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (
Albright, Lisa M., Slatko, Barton E.
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Blue-native PAGE in plants: a tool in analysis of protein-protein interactions [PDF]
Plant Methods, 2005 Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey +2 more
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Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor [PDF]
Journal of Mathematical and Fundamental Sciences, 2019 . Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
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The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]
Biochemical Journal, 1980 The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin
Evans, RW, Williams, J
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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay [PDF]
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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Power and limitations of electrophoretic separations in proteomics strategies [PDF]
Mass Spectrometry Reviews 28, 5 (2009) 816-43, 2009 Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis.
Aebersold +225 more
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The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
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Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]
Pesquisa Veterinária Brasileira, 2021 : Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo +4 more
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A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels
BioTechniques, 2023 Sodium dodecyl sulfate–polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment.
Kristan M Gomez +5 more
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Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (
Ammar Q. Muhammed +2 more
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