Results 11 to 20 of about 705,442 (212)

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

Mass Spectrometry Reviews 28, 5 (2009) 816-43, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry. These techniques, dealing with proteins or peptides, can rely on chromatography or electrophoresis.
Aebersold, An, Andersen, Anderson, Anderson, Anderson, Anderson, Anderson, Appel, Artoni, Baczek, Baczek, Bell, Berggren, Bjellqvist, Bjellqvist, Bonner, Braun, Bravo, Bravo, Bravo, Bravo, Brown, Bunai, Bunger, Burre, Bury, Caglio, Caglio, Camacho-Carvajal, Cantin, Cargile, Cargile, Cargile, Castellanos-Serra, Cejka, Chen, Chevalier, Chevallet, Chiari, Chiari, Chick, Claverol, Cleveland, Cohen, Colinge, Corbett, Coughenour, Courchesne, Cox, Daban, Daban, Davis, Domon, Dukan, Duncan, Egen, Eng, Eravci, Essader, Fernandez-Patron, Figeys, Fritz, Garrels, Garrels, Gelfi, Gelfi, Giometti, Gordon, Gordon, Gorg, Gorg, Gorg, Groleau, Gronow, Han, Hardison, Hartinger, Heller, Henrich, Henzel, Hisabori, Hoffert, Hoffmann, Horowitz, Horth, Hunzinger, James, Johnson, Johnston, Jorgensen, Jovin, Jovin, Kasicka, Keller, Kobayashi, Krijgsveld, Krokhin, Kuroda, Kuster, Kyte, Laemmli, Lambert, Langen, Lasonder, Lengqvist, Liang, Link, Liu, Locke, Loo, Lopez, Lu, Lu, Lu, Luche, Lui, Luo, Luque-Garcia, Lyubimova, Macfarlane, Macfarlane, MacGillivray, Mackintosh, Malmstrom, Malone, Matsudaira, Mazzeo, Mets, Moritz, Nakamura, Navarre, Neuhoff, Nielsen, Novakova, O'Farrell, O'Farrell, Ogorzalek Loo, Orrick, Ortiz, Oses-Prieto, Pappin, Patton, Peng, Perkins, Petritis, Phelps, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Radola, Radola, Rais, Reid, Reynolds, Richert, Rigaut, Righetti, Righetti, Righetti, Righetti, Righetti, Ros, Rosenfeld, Sanchez, Santoni, Schagger, Schagger, Scherl, Schirle, Schluesener, Schmitt, Sevinsky, Sevinsky, Shang, Shapiro, Shen, Shimura, Shimura, Siemankowski, SimoAlfonso, Simpson, Steinberg, Steinberg, Switzer, Szponarski, Szponarski, Tabb, Tan, Tarroux, Tastet, Towbin, Tsugita, Tuszynski, Unlu, van Oostveen, Vonguyen, Voris, Wang, Wang, Washburn, Weber, Wen, West, White, Wilkins, Williams, Wiltfang, Winkler, Wise, Witze, Xiao, Xie, Xie, Yamaguchi, Yamaguchi, Yata, Yates, Young, Yu, Yuan, Zahedi, Zewert, Zischka, Zuo   +225 more
arxiv   +6 more sources

Electrophoretic Separation of the Soluble Protein of Sclerotinia sclerotiorum, S. trifoliorum and S. Minor [PDF]

Journal of Mathematical and Fundamental Sciences, 2019
. Soluble proteins extracted from 3 species of Sclerotinia sclerotiorum, S. trifoliorum and S. minor were separated on polyacrylamide gel electrophoresis. The three species of Sclerotinia could be distinguished by their protein patterns.
Buddy Dani Kosasih
doaj   +2 more sources

Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]

Journal of Proteomics 74, 10 (2011) 1829-41, 2011
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the
##Righetti, ##Righetti, ##Westermeier, #Celis, Afjehi-Sadat, Aicher, Anderson, Appel, Bravo, Bredemeyer, Burre, Cejka, Celis, Chevalier, Chevallet, Chevallet, Chevallet, Corbett, Cécile Lelong, Damerval, Eravci, Garrels, Ghaemmaghami, Gianazza, Gordon, Gorg, Gorg, Gravel, Gronow, Hackett, Harder, Hartinger, Ingolia, Ishigami, Ivanov, John, Karp, Klose, Kondo, Laemmli, Lelong, Lelong, Lovato, Lu, Luche, Luche, Maltese, Mechin, Nakamura, Olsson, Orrick, Perdew, Perluigi, Perrot, Pitarch, Pun, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rasmussen, Richert, Righetti, Sanchez-Campillo, Semple-Rowland, Seo, Sinha, Sobel, Tannu, Tarroux, Tarroux, Taylor, Thierry Rabilloud, Tuszynski, Viswanathan, Wen, Wildgruber, Wildgruber, Yang, Zhan, Zhou, ⁎Gorg, ⁎Gorg, ⁎Hecker, ⁎Miller, ⁎Miller, ⁎Minden, ⁎Patton, ⁎Petrak, ⁎Rabilloud, ⁎Rabilloud, ⁎Rabilloud, ⁎Righetti, ⁎Wang, ⁎Westermeier, ⁎⁎Aebersold, ⁎⁎Anderson, ⁎⁎Anderson, ⁎⁎Bravo, ⁎⁎Gorg, ⁎⁎James, ⁎⁎MacGillivray, ⁎⁎Matsudaira, ⁎⁎Neuhoff, ⁎⁎O'Farrell, ⁎⁎Rabilloud, ⁎⁎Rosenfeld, ⁎⁎Unlu, ⁎⁎Wilkins   +108 more
arxiv   +6 more sources

Blue-native PAGE in plants: a tool in analysis of protein-protein interactions [PDF]

Plant Methods, 2005
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey, Braun Hans-Peter, Eubel Holger   +2 more
doaj   +3 more sources

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő, Dudás, Brigitta, Erdei, Sára, Orosz, László   +3 more
core   +1 more source

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core   +2 more sources

Denaturing Polyacrylamide Gel Electrophoresis [PDF]

, 2000
Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (
Albright, Lisa M., Slatko, Barton E.
core   +1 more source

Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

Nucleic Acids Research, 1981
We describe the use of gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions. The method, which we call protein distribution analysis, is simple, sensitive and yields thermodynamically rigorous
M. Fried, D. Crothers
semanticscholar   +1 more source

Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis

Clinical Proteomics, 2014
Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to ...
Sameh Magdeldin, S. Enany, Y. Yoshida, Bo Xu, Ying Zhang, Zam Zureena, Ilambarthi Lokamani, Eishin Yaoita, Tadashi Yamamoto   +8 more
semanticscholar   +1 more source