Results 271 to 280 of about 517,584 (294)

Gradient SDS Polyacrylamide Gel Electrophoresis

2003
The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 6. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate
openaire   +2 more sources

SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*

Annals of the New York Academy of Sciences, 1964
SummaryCross‐linked homogeneous 5 per cent polyacrylamide gel in rod form is a convenient medium for sharp analytical electrophoretic separation in microgram quantities of most serum proteins and of many biological ampholytes.
openaire   +3 more sources

Preparative electrophoresis on polyacrylamide gel

Analytical Biochemistry, 1964
Nickolas Calvanico, David Racusen
openaire   +3 more sources

Neutral Polyacrylamide Gel Electrophoresis

Cold Spring Harbor Protocols, 2006
Joseph Sambrook, David W. Russell
openaire   +3 more sources

SDS Polyacrylamide Gel Electrophoresis of Proteins

1996
Crosslinked polyacrylamide gels are formed from the polymerization of acrylamide monomer in the presence of smaller amounts of N,N'-methylene-bis-acrylamide (normally referred to as “bis-acrylamide”) (Fig. 1). Note that bis-acrylamide is essentially two acrylamide molecules linked by a methylene group and is used as a crosslinking agent.
openaire   +2 more sources

Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels

2003
For the majority of purposes, such as restriction mapping and sizing of cloned fragments, electrophoresis of DNA in horizontal agarose gels is perfectly adequate. As the size of the fragments of interest decreases to below 1000 base pairs (bp), however, the resolution deteriorates significantly, and an alternative is required.
openaire   +3 more sources

Preparative Polyacrylamide-Gel Electrophoresis [PDF]

Biochemical Society Transactions, 1973
openaire   +1 more source

Polyacrylamide gel electrophoresis of DNA

Analytical Biochemistry, 1972
openaire   +3 more sources

[16] Preparative polyacrylamide gel electrophoresis

1983
Irvin Isenberg, Steven Spiker
openaire   +3 more sources

SDS polyacrylamide gel electrophoresis

Trends in Biochemical Sciences, 2000
openaire   +3 more sources