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Gradient SDS Polyacrylamide Gel Electrophoresis
2003The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 6. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate
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Polyacrylamide Temperature Gradient Gel Electrophoresis
2013Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins.
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Preparative electrophoresis on polyacrylamide gel
Analytical Biochemistry, 1964D, RACUSEN, N, CALVANICO
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SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*
Annals of the New York Academy of Sciences, 1964SummaryCross‐linked homogeneous 5 per cent polyacrylamide gel in rod form is a convenient medium for sharp analytical electrophoretic separation in microgram quantities of most serum proteins and of many biological ampholytes.
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Neutral Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
2003For the majority of purposes, such as restriction mapping and sizing of cloned fragments, electrophoresis of DNA in horizontal agarose gels is perfectly adequate. As the size of the fragments of interest decreases to below 1000 base pairs (bp), however, the resolution deteriorates significantly, and an alternative is required.
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SDS polyacrylamide gel electrophoresis
Trends in Biochemical Sciences, 2000openaire +2 more sources