Results 51 to 60 of about 705,442 (212)
Two-dimensional gel electrophoresis in proteomics: past, present and future [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist.
arxiv +1 more source
Formation and quantification of protein complexes between peroxisomal alcohol oxidase and GroEL [PDF]
, 1992 We have studied the use of yeast peroxisomal alcohol oxidase (AO) as a model protein for in vitro binding by GroEL. Dilution of denatured AO in neutral buffer leads to aggregation of the protein, which is prevented by the addition of GroEL.
Distel +25 more
core +3 more sources
Variations on a theme: Changes to electrophoretic separations that can make a difference [PDF]
Journal of proteomics (2010) epub ahead of print, 2010 Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
arxiv +1 more source
Study of total seed storage protein in indigenous Brassica species based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) [PDF]
, 2016 Genetic diversity was studied in 234 accessions of locally collected Brassica species for total seed protein content through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). These accessions were collected from different locations of
Akmal, M +6 more
core +2 more sources
BMC Research Notes, 2021 Objective The advancement of molecular techniques in an era in which high-throughput sequencing has revolutionized biology renders old-fashioned alternatives to high-throughput methods obsolete.
Julissa J. Sánchez-Velásquez +3 more
doaj +1 more source
Electrophoretic separation of proteins [PDF]
, 1981 There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins.
Ravindranath, M H, Subhashini, M H
core
Purification and characterization of putrescine synthase from cucumber seedlings. A multifunctional enzyme involved in putrescine biosynthesis [PDF]
, 1986 The multifunctional enzyme, putrescine synthase has been purified fromCucumis sativus and characterized. This enzyme harbours agmatine iminohydrolase, ornithine transcarbamylase, putrescine transcarbamylase and carbamate kinase activities, whose ...
Adiga, P. R., Prasad, G. L.
core +1 more source
Acta Societatis Botanicorum Poloniae, 2015 SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and ...
Aleksander F. Sikorski
doaj +1 more source
The Whereabouts of 2D Gels in Quantitative Proteomics [PDF]
Methods in Molecular Biology -Clifton then Totowa- 893 (2012)
25-35, 2012 Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series.
arxiv +1 more source
Activity staining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis [PDF]
, 2005 A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were ...
Chhatpar, HS, Gohel, Vipul, Vyas, Pranav
core +2 more sources