Power and limitations of electrophoretic separations in proteomics strategies [PDF]
, 2009 Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold +225 more
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Use of capillary electrophoresis as a method development tool for classical gel electrophoresis [PDF]
, 1998 Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan.
Gratz, Samuel R. +2 more
core +1 more source
Two-dimensional SDS-PAGE fractionation of biological samples for biomarker discovery [PDF]
Methods in Molecular Biology -Clifton then Totowa- 1002 (2013)
151-65, 2013 Two-dimensional electrophoresis is still a very valuable tool in proteomics, due to its reproducibility and its ability to analyze complete proteins. However, due to its sensitivity to dynamic range issues, its most suitable use in the frame of biomarker discovery is not on very complex fluids such as plasma, but rather on more proximal, simpler fluids
arxiv +1 more source
ELECTROPHORESIS OF PEPSIN [PDF]
Journal of General Physiology, 1940 1. A number of pepsin solutions containing several protein components have been studied by the electrophoresis method. All samples show a homogeneous boundary moving to the anode at pH 4.4. 2. The activity of this material may be higher than that of the original solution on the basis of total nitrogen but is the same as that of the ...
Roger M. Herriott +2 more
openaire +3 more sources
Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]
, 2008 QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core +2 more sources
Nonlinear electrophoresis of dielectric and metal spheres in a nematic liquid crystal [PDF]
Nature 467 (2010), 947-950, 2011 Electrophoresis is a motion of charged dispersed particles relative to a fluid in a uniform electric field. The effect is widely used to separate macromolecules, to assemble colloidal structures, to transport particles in nano- and micro-fluidic devices and displays.
arxiv +1 more source
Electromembrane extraction and capillary electrophoresis with capcitively coupled contactless conductivity detection: multi-extraction capabilities to analyse trace metals from saline samples [PDF]
, 2018 This is the pre-peer reviewed version of the following article: Silva, M., Mendiguchía, C., Moreno, C. and Kubáň, P. (2018), Electromembrane extraction and capillary electrophoresis with capacitively coupled contactless conductivity detection: Multi ...
Kuban, Pavel +3 more
core +1 more source
Microsecond electrophoresis [PDF]
Proceedings of the National Academy of Sciences, 2003 Although analysis strategies exist for probing a diverse array of molecular properties, most of these approaches are not amenable to the study of reaction intermediates and other transient species. Separations in particular can provide detailed information on attributes not readily measured by spectroscopy but typically are performed over ...
Matthew L. Plenert, Jason B. Shear
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An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]
IEEE Electro/Information Technology, 2007, 2016 Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv +1 more source
On the importance of hydrodynamic interactions in polyelectrolyte electrophoresis [PDF]
, 2008 The effect of hydrodynamic interactions on the free-solution electrophoresis of polyelectrolytes is investigated with coarse-grained molecular dynamics simulations. By comparing the results to simulations with switched-off hydrodynamic interactions, we demonstrate their importance in modelling the experimentally observed behaviour. In order to quantify
arxiv +1 more source