Results 41 to 50 of about 192,083 (323)

A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development

STAR Protocols, 2021
Summary: In vivo electroporation has become a key technique to study genetic mechanisms of brain development. However, electroporation of the embryonic pallium in oviparous species, interesting for evolutionary studies but distinct from in utero ...
Adrián Cárdenas, Víctor Borrell
doaj   +1 more source

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts

The Journal of Reproduction and Development, 2019
The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is
Fuminori TANIHARA, Maki HIRATA, Shigeki MORIKAWA, Nhien Thi NGUYEN, Quynh Anh LE, Takayuki HIRANO, Yoshiyuki FUKUMI, Toshiaki ABE, Takeshige OTOI   +8 more
doaj   +1 more source

Single cell electroporation on chip [PDF]

, 2006
In this thesis the results of the development of microfluidic cell trap devices for single cell electroporation are described, which are to be used for gene transfection.
Valero, Ana
core   +2 more sources

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method

Journal of Experimental Life Science, 2017
Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation.
Diana Aisyah, Agoes Soeprijanto, Yuni Kilawati   +2 more
doaj   +1 more source

A Study on Nonthermal Irreversible Electroporation of the Thyroid. [PDF]

, 2019
BackgroundNonthermal irreversible electroporation is a minimally invasive surgery technology that employs high and brief electric fields to ablate undesirable tissues.
Huang, Jianwei, Lv, Yanpeng, Rubinsky, Boris, Wang, Yunlong, Zhang, Yanfang   +4 more
core  

Functional Materials for Environmental Energy Harvesting in Smart Agriculture via Triboelectric Nanogenerators

Advanced Functional Materials, EarlyView.
This review explores functional and responsive materials for triboelectric nanogenerators (TENGs) in sustainable smart agriculture. It examines how particulate contamination and dirt affect charge transfer and efficiency. Environmental challenges and strategies to enhance durability and responsiveness are outlined, including active functional layers ...
Rafael R. A. Silva, Giulio Fatti, Emanuel Carlos, Guilherme Ferreira, Sumita Goswami, Suman Nandy, Luiz H. C. Mattoso, Elvira Fortunato, Caio G. Otoni, Rodrigo Martins   +9 more
wiley   +1 more source

Expression kinetics and innate immune response after electroporation and LNP-mediated delivery of a self-amplifying mRNA in the skin [PDF]

, 2019
In this work, we studied the expression kinetics and innate immune response of a self-amplifying mRNA (sa-RNA) after electroporation and lipid-nanoparticle (LNP)-mediated delivery in the skin of mice. Intradermal electroporation of the sa-RNA resulted in
De Temmerman, Joyca, Gitsels, Arlieke, Huysmans, Hanne, Mc Cafferty, Séan, Mui, Barbara L, Sanders, Niek, Tam, Ying K, Vanrompay, Daisy, Zhong, Zifu   +8 more
core   +2 more sources