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Transcription elongation complex: structure and function
Current Opinion in Microbiology, 2001Our understanding of the mechanisms of transcription has been greatly advanced by recent determination of the X-ray structure of bacterial RNA polymerase. Using crosslinking approaches, extensive mapping of DNA and RNA contacts onto this structure allowed tracking of the path of nucleic acids through the transcription elongation complex.
N, Korzheva, A, Mustaev
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Structure of Transcription Elongation Complexes in Vivo
Science, 1992The opening of duplex DNA in the elongation phase of transcription by Escherichia coli RNA polymerase in vivo was detected at a regulatory site where a prolonged pause in transcription occurs. Single-stranded DNA in the transcription bubble was identified by its reactivity with potassium permanganate (KMnO
M, Kainz, J, Roberts
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Stereochemistry of the elongation factor Tu . GTP complex
European Journal of Biochemistry, 1983The geometry of the Me2+. GTP complex at the active site of EF-Tu from Bacillus stearothermophilus has been investigated using thiophosphate analogs of GTP to inhibit the kirromycin-induced GTPase reaction at 60 mM NH4Cl. There is no reversed selectivity for the diastereomers (Rp and Sp) of guanosine 5'-O-(1-thiotriphosphate) (GTP[alpha S]) on ...
Leupold, C., Goody, R., Wittinghofer, A.
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A coordinated codon-dependent regulation of translation by Elongator
Cell Cycle, 2012 More than a decade ago, the purification of the form of the RNA polymerase II (PolII) engaged in elongation led to the discovery of an associated, multi-subunit (Elp1-6) complex named “Elongator” by the Svejstrup lab.
Damien Hermand, Fanelie France Bauer
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Structure of human RNA polymerase III elongation complex
Cell Research, 2021RNA polymerase III (Pol III) transcribes essential structured small RNAs, such as tRNAs, 5S rRNA and U6 snRNA. The transcriptional activity of Pol III is tightly controlled and its dysregulation is associated with human diseases, such as cancer. Human Pol III has two isoforms with difference only in one of its subunits RPC7 (α and β).
Liang Li +5 more
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Crystallization of the yeast elongation factor complex eEF1A–eEF1Bα
Acta Crystallographica Section D Biological Crystallography, 2001Crystals of the Saccharomyces cerevisiae elongation factor eEF1A (formerly EF-1 alpha) in complex with a catalytic C-terminal fragment of the nucleotide-exchange factor eEF1B alpha (formerly EF-1 beta) were grown by the sitting-drop vapour-diffusion technique, using polyethylene glycol 2000 monomethyl ether as precipitant.
Pedersen, Lise +4 more
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Elongation factors complex in plant cells
Plant Science Letters, 1975Abstract By gel filtration and density gradient centrifugation the elongation factor 1 (EF1)-elongation factor 2(EF2) complex was detected in moistened wheat germ. The sedimentation coefficient of the complex was determined as 7.3 S. On the basis of its biological properties it is suggested that the EF1-EF2 complex represents the native form of the ...
T. Twardowski, A.B. Legocki
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Origin and Evolution of Transcription Elongation Complexes
Биофизика / BiophysicsIn the process of cellular transcription, short-lived intermediates called elongation complexes are formed. They contain matrix DNA, RNA, DNA-dependent RNA polymerase, and transcription factors that regulate transcription parameters. The accumulated biochemical and structural data on transcriptional elongation complexes allow us to consider them as ...
E. V. Osina +4 more
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Le complexe protéique Elongator
médecine/sciences, 2023Vicente José Planelles-Herrero +1 more
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