Results 11 to 20 of about 452,413 (312)

Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization

Scientific Reports, 2022
Somatic cell nuclear transfer technique (SCNT) has proved to be an outstanding method of multiplication of elite animals but accompanied with low efficiency and live birth rate of cloned animals. Epigenetic alterations of DNA has been one of the culprits
Shivani Malpotra, Pallavi Goel, Songyukta Shyam, Manoj Kumar Singh, Prabhat Palta   +4 more
doaj   +1 more source

Oxamflatin and ascorbic acid improves developmental competence and quality of buffalo (Bubalis bubalis) cloned embryos

Revista Científica, 2023
Buffalo cloning is a powerful assisted reproductive tool for multiplying elite buffalo germplasm. However, the live off-spring production efficiency is low due to aberrant epigenetic reprogramming.
Sonal Gupta, Gaurav Tripathi, Kartikey Patel, Ram Parsad, Tanya Gupta, Naresh L. Selokar, Manoj Kumar Singh   +6 more
doaj   +1 more source

CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3′ UTR induce graying in rabbit

Scientific Reports, 2017
The 3′ untranslated regions (UTRs), located at the end of mRNA molecules, are believed to play a role in RNA replication and/or protein translation. Mutations in the tyrosinase (Tyr) gene are known to cause recessive albinism in humans and other species.
Yuning Song, Yuxin Xu, Jichao Deng, Mao Chen, Yi Lu, Yong Wang, Haobin Yao, Lina Zhou, Zhiquan Liu, Liangxue Lai, Zhanjun Li   +10 more
doaj   +1 more source

Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells

Molecular Therapy: Methods & Clinical Development, 2016
Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing tools, but the ability to divulge functions of new genes have not increased correspondingly.
Abul Usmani, Nirmalya Ganguli, Subodh K Jain, Nilanjana Ganguli, Rajesh Kumar Sarkar, Mayank Choubey, Mansi Shukla, Hironmoy Sarkar, Subeer S Majumdar   +8 more
doaj   +1 more source

Prognostic Values From Integrated Analysis of the Nomogram Based on RNA-Binding Proteins and Clinical Factors in Endometrial Cancer

Clinical Medicine Insights: Oncology, 2022
Background: Endometrial cancer (EC) is a common gynecological malignancy, and the prognosis of advanced EC is unsatisfactory. The deregulated expression of RNA-binding proteins (RBPs) is closely associated with the occurrence and development of cancer ...
Shuang Yuan, Xiao Sun, Lihua Wang
doaj   +1 more source

Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation

Scientific Reports, 2017
Spermatogenesis can be affected by nutrition, which operates through normal physiological processes by changing the testicular mass and hormone levels profoundly.
Yanli Zhang, Hua Yang, Le Han, Fengzhe Li, Tingting Zhang, Jing Pang, Xu Feng, Caifang Ren, Shengyong Mao, Feng Wang   +9 more
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Semen quality and fertility effectiveness of cloned buffalo (Bubalus bubalis) bulls

Revista Científica, 2023
In India, due to a severe shortage of elite bulls, semen available from progeny-tested bulls covers a limited breedable population of buffalo. Animal cloning has proved beneficial in making identical genetic copies in the shortest possible time to meet ...
Manoj Kumar Singh, Smiriti Gupta, Kanika Gandhi, S.S. Lathwal, Naresh L. Selokar   +4 more
doaj   +1 more source

IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche

Nature Communications, 2020
The signals regulating the establishment and maintenance of the pluripotent epiblast in human embryos are unclear. Here, the authors use a bioinformatics approach to identify the role of IGF1 in human embryo development, and from this, propose a culture ...
Sissy E. Wamaitha, Katarzyna J. Grybel, Gregorio Alanis-Lobato, Claudia Gerri, Sugako Ogushi, Afshan McCarthy, Shantha K. Mahadevaiah, Lyn Healy, Rebecca A. Lea, Miriam Molina-Arcas, Liani G. Devito, Kay Elder, Phil Snell, Leila Christie, Julian Downward, James M. A. Turner, Kathy K. Niakan   +16 more
doaj   +1 more source

Fixed versus flexible antagonist protocol in women with predicted high ovarian response except PCOS: a randomized controlled trial

BMC Pregnancy and Childbirth, 2021
Background No previous study directly compares the fixed day-5 initiation versus the flexible initiation of GnRH antagonist administration in IVF/ICSI for those patients who are predicted as high ovarian responders without PCOS.
Xiu Luo, Li Pei, Fujie Li, Chunli Li, Guoning Huang, Hong Ye   +5 more
doaj   +1 more source

An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm [PDF]

Veterinary World, 2017
Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X-
Shiv Kumar Yadav, Dharmendra Kumar Gangwar, Jarnail Singh, Chiranjeev Kumar Tikadar, V. Vinoth Khanna, Sudha Saini, Sunny Dholpuria, Prabhat Palta, Radhey Shyam Manik, Manoj Kumar Singh, Suresh Kumar Singla   +10 more
doaj   +1 more source