Results 61 to 70 of about 254,276 (267)

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification

eLife
The first lineage allocation in mouse and human embryos separates the inner cell mass (ICM) from the outer trophectoderm (TE). This symmetry-breaking event is executed through polarization of cells at the 8 cell stage and subsequent asymmetric divisions,
Adiyant Lamba, Meng Zhu, Maciej Meglicki, Sylwia Czukiewska, Lakshmi Balasubramaniam, Ron Hadas, Nina Weishaupt, Ekta M Patel, Yu Hua Kavanagh, Ran Wang, Naihe Jing, Magdalena Zernicka-Goetz   +11 more
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Discovery of differentially expressed lncRNAs in porcine ovaries with smaller and bigger litter size

Frontiers in Genetics
IntroductionThe number of litters is an important reproductive trait, which is one of the main indicators reflecting the production level and economic benefit of the pig farm.
Saixing Duan, Falei Li, Libing Meng, Shimei Cheng, Huangqi Shi, Yingjie Qu, Chaoyue He, Shengyu Gao, Jian Li, Shiduo Sun, Yong Liu, Gaoxiao Xu, Gaoxiao Xu   +12 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Birth of India’s first cloned cattle and analysis of its health and reproduction status: A case study

Scientific Reports
Somatic cell nuclear transfer (SCNT) allows the multiplication of elite livestock and conservation of endangered species. However, restrictions on cow slaughter limit the access to oocytes for SCNT applications in Indian cattle breeds.
Naresh L. Selokar, Manoj K. Singh, Gaurav Tripathi, Kartikey Patel, Ranjeet Verma, Priyanka Singh, Ajay Aswal, Manmohan Singh Chauhan   +7 more
doaj   +1 more source

Bisphenol P induces ovarian dysfunction by directly targeting PTGS2 and disrupting steroid hormone synthesis

Ecotoxicology and Environmental Safety
Emerging evidence suggests structural analogues of bisphenol A (BPA), including the increasingly used substitute bisphenol P (BPP), pose substantial reproductive risks. However, BPP's ovarian toxicity remains uncharacterized.
Dingmei Qin, Zijian Ma, Zhipeng Lin, Lei Wang, Jian Wang, Xing Zhang, Xiaoqing Wu, Jingya Li, Shujun Sun, Shangrong Zhang   +9 more
doaj   +1 more source

Developmental, Neuroanatomical and Cellular Expression of Genes Causing Dystonia

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Dystonia is one of the most common movement disorders, with variants in multiple genes identified as causative. However, an understanding of which developmental stages, brain regions, and cell types are most relevant is crucial for developing relevant disease models and therapeutics.
Darren Cameron, Nicholas E. Clifton, Daniel Cabezas de la Fuente, Peter Holmans, Nicholas J. Bray, Kathryn J. Peall   +5 more
wiley   +1 more source