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2012
The embryonic stem cell test is an animal-free alternative test method for developmental toxicity. Mouse embryonic stem cells are cultured in a hanging drop method to form embryoid bodies. These embryoid bodies, when plated on tissue culture dishes, differentiate to form contracting myocardial cell foci within 10 days.
Sjors H.W. Schulpen, Aldert H. Piersma
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The embryonic stem cell test is an animal-free alternative test method for developmental toxicity. Mouse embryonic stem cells are cultured in a hanging drop method to form embryoid bodies. These embryoid bodies, when plated on tissue culture dishes, differentiate to form contracting myocardial cell foci within 10 days.
Sjors H.W. Schulpen, Aldert H. Piersma
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METTL3 regulates heterochromatin in mouse embryonic stem cells
Nature, 2021Wenqi Xu +13 more
semanticscholar +1 more source
2006
Bovine embryonic stem (bES) cell lines reported to date vary in morphology and marker expression, such as alkaline phosphatase (ALPL), stage-specific embryonic antigen 4 (SSEA4), and octamer-binding transcription factor-4 (OCT4), that normally are associated with the undifferentiated, pluripotent state.
Marsha L. Roach +3 more
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Bovine embryonic stem (bES) cell lines reported to date vary in morphology and marker expression, such as alkaline phosphatase (ALPL), stage-specific embryonic antigen 4 (SSEA4), and octamer-binding transcription factor-4 (OCT4), that normally are associated with the undifferentiated, pluripotent state.
Marsha L. Roach +3 more
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Mutation in Embryonic Stem Cells
Science, 2001A single human embryonic stem (es) cell might be modified in the laboratory to provide an unlimited supply of cells for therapy, according to Geron researcher Melissa Carpenter in the News Focus article “Stem cells: new excitement, persistent questions” (G. Vogel, 1 Dec., p.
Philip Rappa +2 more
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2006
Embryonic stem (ES) cells are derived from preimplantation stage mouse embryos at the time when they have reached the blastocyst stage. It is at this point that the first steps of differentiation take place during mammalian embryonic development.
Kristina Vintersten, Andras Nagy
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Embryonic stem (ES) cells are derived from preimplantation stage mouse embryos at the time when they have reached the blastocyst stage. It is at this point that the first steps of differentiation take place during mammalian embryonic development.
Kristina Vintersten, Andras Nagy
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Differentiation of Embryonic Stem Cells
Current Protocols in Neuroscience, 1997AbstractEmbryonic stem (ES) cells are pluripotent cells whose developmental state is equivalent to cells of the inner cell mass in the blastocyst‐stage embryo. This unit presents a method of producing differentiated ES cells in which the cells are first aggregated on a less adhesive surface to form embryoid bodies (EBs).
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2006
Embryonic development prior to implantation of the embryo in the uterus includes a stage of blastocyst formation, where the initial mass of cells is segregated into an inner cell mass destined to contribute to the developing embryo and an outer layer of cells that will contribute to the fetal portion of the placenta.
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Embryonic development prior to implantation of the embryo in the uterus includes a stage of blastocyst formation, where the initial mass of cells is segregated into an inner cell mass destined to contribute to the developing embryo and an outer layer of cells that will contribute to the fetal portion of the placenta.
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Epigenetics of Embryonic Stem Cells
2012Understanding the molecular mechanisms involved in the control of cell differentiation during embryonic development is currently one of the main objectives of developmental biology. This knowledge will provide a basis for the development of new strategies in the field of regenerative medicine, one of the most promising weapons to fight many human ...
Vincenzo Calvanese, Mario F. Fraga
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A Bivalent Chromatin Structure Marks Key Developmental Genes in Embryonic Stem Cells
Cell, 2006B. Bernstein +14 more
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2010
Stem cells can be isolated from a variety of sources and they are typically classified based on their tissue of origin. Embryonic stem cells are, as the name indicates, derived from the inner cell mass of pre-implantation stage blastocysts at day 5–7 post fertilisation.
Mikael C.O. Englund +2 more
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Stem cells can be isolated from a variety of sources and they are typically classified based on their tissue of origin. Embryonic stem cells are, as the name indicates, derived from the inner cell mass of pre-implantation stage blastocysts at day 5–7 post fertilisation.
Mikael C.O. Englund +2 more
openaire +2 more sources