Results 51 to 60 of about 62,727 (249)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Morphological spectrum of gamma rays and ems induced viable mutants in cowpea (Vigna unguiculata (L.) Walp)

Electronic Journal of Plant Breeding, 2020
Seeds of cowpea (Vigna unguiculata (L.) Walp) variety CO 7 and Tirunelveli local were treated with gamma ray doses at 150, 200, 250, 300 and 350 Gy and EMS (Ethyl Methane Sulphonate) doses at 5, 10, 15, 20 and 25 mM.
M. Preethi and A. Muthuswamy
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Exploratory Analysis of ELP1 Expression in Whole Blood From Patients With Familial Dysautonomia

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Familial dysautonomia (FD) is a hereditary neurodevelopmental disorder caused by aberrant splicing of the ELP1 gene, leading to a tissue‐specific reduction in ELP1 protein expression. Preclinical models indicate that increasing ELP1 levels can mitigate disease manifestations.
Alejandra González‐Duarte, Lucy Norcliffe‐Kaufmann, Maria Luisa Cotrina, Zenith Khan, Kaia Dalamo, Patricio Millar Vernetti, Matthew Lawless, Elisabetta Morini, Monica Salani, Marla Weetall, Jana Narasimhan, Agostino G. Rocha, Susan A. Slaugenhaupt, Horacio Kaufmann   +13 more
wiley   +1 more source

Ambulance traffic accidents and their impact on prehospital personnel: a mixed-methods study

Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
Objective Prehospital personnel operate in environments with a significant risk of on-duty traffic accidents. We investigated the prevalence and characteristics of on-duty ambulance traffic accidents and explored how prehospital personnel perceive and ...
Marianne Winther, Oliver Beierholm Sørensen, Anne Craveiro Brøchner, Daniel Wittrock, Peter Martin Hansen, Jens Stubager Knudsen, Lisa Seest Nielsen, Signe Amalie Wolthers, Lars Bak Nielsen, Helle Collatz Christensen, Lotte Huniche, Søren Mikkelsen, Eva Laerkner   +12 more
doaj   +1 more source

In‐Depth Profiling Highlights the Effect of Efgartigimod on Peripheral Innate and Adaptive Immune Cells in Myasthenia Gravis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Myasthenia gravis (MG) is an autoimmune disorder characterized by antibody‐mediated complement activation. Efgartigimod, a neonatal Fc receptor (FcRn) antagonist, is approved for treating generalized MG (gMG). However, its modulatory effects on upstream innate and adaptive immune cells remain largely unexplored.
Lei Jin, Liu Dong, Ying Wang, Dingxian He, Chong Yan, Jianying Xi, Haoqin Jiang, Yu Shi, Ming Guan, Chongbo Zhao, Jie Song, Sushan Luo   +11 more
wiley   +1 more source

Sustainable Ceramics: Creating Effective Key Performance Indicators for Industry Monitoring [PDF]

E3S Web of Conferences
Sustainable development in the ceramic industry focuses on meeting present needs without compromising future resources. Key strategies include recycling waste, improving energy efficiency, and adopting green technologies.
Sharma Manni, Vani V. Divya, Helena Raj J. Vijilius, Dutt Amit, Kumar J. Sunil, Almusawi Muntather, Gupta Nakul, Goyal Rajesh   +7 more
doaj   +1 more source

Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz, Christopher H. Thompson, Linda C. Laux, Alfred L. George Jr.   +3 more
wiley   +1 more source

Evidence of Iron Accumulation in Cerebral Adrenoleukodystrophy: A Potential Novel Disease Mechanism

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT In this first application of Quantitative Susceptibility Mapping Source Separation to cerebral adrenoleukodystrophy, we uncovered alterations in iron and myelin within lesions and normal appearing white matter. As validation, we demonstrate abnormal iron accumulation in those same compartments within primary brain tissue.
Christina L. Nemeth, Mert Sisman, Jinwei Zhang, Hyeong‐geol Shin, Xu Li, Bela Turk, Ali Fatemi, Thanh Nguyen, Eric Mallack   +8 more
wiley   +1 more source

Socioeconomic Disparities in Prehospital Emergency Care in a Danish Tax-Financed Healthcare System: Nationwide Cohort Study

Clinical Epidemiology, 2022
Juliane Frydenlund,1 Julie Mackenhauer,1 Erika F Christensen,2– 4 Helle Collatz Christensen,5 Ulla Væggemose,6,7 Jacob Steinmetz,8,9 Søren Paaske Johnsen1 1Department of Clinical Medicine, Aalborg University, Aalborg East, 9220, Denmark; 2Department of ...
Frydenlund J, Mackenhauer J, Christensen EF, Christensen HC, Væggemose U, Steinmetz J, Johnsen SP   +6 more
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