Results 251 to 260 of about 8,672,266 (315)

Selective Dual Apical Surgery for Adjacent Immature and Mature Upper Incisors With a Large Periapical Lesion: A 4-Year Follow-Up Case Report. [PDF]

Clin Case Rep
Asgary S.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Evaluating Improvement in Pain and Sensory Function When Using High-Frequency (10-kHz) Spinal Cord Stimulation in Individuals With Painful Diabetic Neuropathy: A Multicenter Randomized Controlled Trial (PDN-Sensory). [PDF]

Diabetes Care
Hurley RW, Siraj ES, Sills S, Engle M, Johnson C, Pop-Busui R, Armstrong D, Boulton A, Levy B, Tesfaye S, Grunberger G, Petersen E, Azalde R, Edgar D, Nakhle S, Yu C, Khan K, Skaribas I, Amirdelfan K, Nguyen Q, Jameson J, Guirguis M, Bradley K, Patel JS, Bintrim DJ, Caraway D.   +25 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Assessing the role of plan complexity and target geometry through multi-institutional gel based end-to-end QA in multi-focal single isocenter stereotactic radiosurgery. [PDF]

J Appl Clin Med Phys
Stasinou D, Platoni K, Margaroni V, Zourari K, Zoros E, Papanikolaou N, Pappas E.   +6 more
europepmc   +1 more source

Shifting Public Discourse on Life-Sustaining Treatment in South Korea From 2014 to 2025: Longitudinal Social Media Analysis. [PDF]

JMIR Infodemiology
Kim M, Suh HS.
europepmc   +1 more source