Fluorescence-based incision assay for human XPF-ERCC1 activity identifies important elements of DNA junction recognition [PDF]
, 2012 The structure-specific endonuclease activity of the human XPF–ERCC1 complex is essential for a number of DNA processing mechanisms that help to maintain genomic integrity.
Bowles, M. +5 more
core +1 more source
Cell Reports, 2017 Iron metabolism is regulated by transcriptional and post-transcriptional mechanisms. The mRNA of the iron-controlling gene, transferrin receptor 1 (TfR1), has long been believed to be negatively regulated by a yet-unidentified endonuclease. Here, we show
Masanori Yoshinaga +8 more
doaj +1 more source
Acta Biochimica et Biophysica Sinica, 2022 Prokaryotic Argonaute (pAgo) nucleases with precise DNA cleavage activity show great potential for gene manipulation. Extensive biochemical studies have revealed that recognition of guides with different 5′ groups by Ago is important for biocatalysis ...
Sun Yingying +6 more
doaj +1 more source
Dna2 is a structure-specific nuclease, with affinity for 5'-flap intermediates [PDF]
, 2010 Dna2 is a nuclease/helicase with proposed roles in DNA replication, double-strand break repair and telomere maintenance. For each role Dna2 is proposed to process DNA substrates with a 5'-flap.
Aguilera +54 more
core +2 more sources
The Human Pregnancy-Specific Glycoprotein Genes are Tightly Linked on the Long Arm of Chromosome 19 and are Coordinately Expressed [PDF]
, 1990 The pregnancy-specific glycoprotein (PSG) genes encode a group of proteins which are found in large amounts in placenta and maternal serum. In situ hybridization analyses of metaphase chromosomes reveal that all the human pregnancy-specific glycoprotein (
Barnett +40 more
core +1 more source
A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
, 2015 Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
core +2 more sources
Human MutLγ, the MLH1–MLH3 heterodimer, is an endonuclease that promotes DNA expansion
Proceedings of the National Academy of Sciences of the United States of America, 2020 Significance Triplet repeat expansion causes multiple neurological disorders, but the mechanisms of triplet repeat expansion are not well understood.
Lyudmila Y Kadyrova +4 more
semanticscholar +1 more source
Human MUS81: A Fence-Sitter in Cancer
Frontiers in Cell and Developmental Biology, 2021 MUS81 complex, exhibiting endonuclease activity on specific DNA structures, plays an influential part in DNA repair. Research has proved that MUS81 is dispensable for embryonic development and cell viability in mammals.
Sisi Chen +7 more
doaj +1 more source
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
Nature, 2014 The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA–DNA base-pairing to target foreign DNA in bacteria.
S. H. Sternberg +4 more
semanticscholar +1 more source
Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]
, 1992 A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner +3 more
core +1 more source