Results 51 to 60 of about 145,931 (267)
International Journal of Electrochemical Science, 2019 DNA methylation is one of the most studied epigenetic modifications of DNA. The methylation at C-5 position of cytosine has been proved to be related to a wide range of biological phenomena. Therefore, it is very important to develop a simple, sensitive,
Ning Xia, Yuanqiang Hao
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Advanced Science, EarlyView.In this study, a MXene‐based V4C3 nanoplatform, VARH, was constructed, which achieved a photothermal conversion efficiency of 44.21% in the NIR‐II window. This platform integrated photothermal therapy, chemodynamic therapy, and thermodynamic therapy with CRISPR/Cas9‐mediated heat shock protein 90 knock‐out technology.
Zi‐Jian Huang +8 more
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International Journal of Electrochemical Science, 2019 In this contribution, Hpa II restriction endonuclease is used for the first time to design endonuclease based hairpin aptamer probe and develop background current eliminated electrochemical aptasensor for sensitive signal-on detection of protein ...
Songbai Zhang +4 more
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Advanced Science, EarlyView.In colorectal cancer, high expression of the DNA repair protein EEPD1 correlates with immune exclusion and poor prognosis. This study demonstrates that EEPD1 depletion induces genomic instability, leading to cytosolic DNA accumulation and subsequent activation of the cGAS‐STING‐type I interferon pathway. This cascade remodels the tumor microenvironment
Liyun Huo +8 more
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Programmable RNA N6,2´‐O‐Dimethyladenosine Editing
Advanced Science, EarlyView.ABSTRACT N6,2’‐O‐dimethyladenosine (m6Am) is a prevalent RNA modification located at the first transcribed nucleotide adjacent to the 5′ cap of mRNAs, where it has been implicated in gene regulation. However, the lack of methods for precise, transcript‐specific manipulation of m6Am has limited its functional dissection.
Yang Li +9 more
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Conserved Endonuclease Function of Hantavirus L Polymerase
Viruses, 2016 Hantaviruses are important emerging pathogens belonging to the Bunyaviridae family. Like other segmented negative strand RNA viruses, the RNA-dependent RNA polymerase (RdRp) also known as L protein of hantaviruses lacks an intrinsic “capping activity ...
Sylvia Rothenberger +4 more
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The Cap-Snatching SFTSV Endonuclease Domain Is an Antiviral Target
Cell Reports, 2020 Summary: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne virus with 12%–30% case mortality rates and is related to the Heartland virus (HRTV) identified in the United States.
Wenjie Wang +11 more
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Advanced Science, EarlyView.ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
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Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis
Advanced Science, EarlyView.In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
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Splicing Endonuclease Is an Important Player in rRNA and tRNA Maturation in Archaea
Frontiers in Microbiology, 2020 In all three domains of life, tRNA genes contain introns that must be removed to yield functional tRNA. In archaea and eukarya, the first step of this process is catalyzed by a splicing endonuclease.
Thandi S. Schwarz +17 more
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