Results 131 to 140 of about 60,825 (304)

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9

Nature Communications
Domain expansion contributes to diversification of RNA-guided-endonucleases including Cas9. However, it remains unclear how REC domain expansion could benefit Cas9.
Shuliang Gao, Benson Weng, Douglas Wich, Liam Power, Mengting Chen, Huiwen Guan, Zhongfeng Ye, Chutian Xu, Qiaobing Xu   +8 more
doaj   +1 more source

Mapping the Conformational Landscape of DNA Minicircles Through Atomic Force Microscopy and Shape Space Analysis

Small, EarlyView.
An automated framework is presented for quantifying DNA conformational variability from atomic force microscopy (AFM) images. DNA backbones are reduced to spline representations and analyzed using cyclic Procrustes analysis to measure shape similarity across ensembles.
Laura Wiggins, Tobias A. Firth, Max C. Gamill, Thomas E. Catley, Agnes Noy, Jonathan M. Fogg, Lynn Zechiedrich, Alice L. B. Pyne   +7 more
wiley   +1 more source

SURVEY AND SUMMARY Diversity of Type II restriction endonucleases that require two DNA recognition sites

, 2003
Orthodox Type IIP restriction endonucleases, which are commonly used in molecular biological work, recognize a single palindromic DNA recognition sequence and cleave within or near this sequence.
Detlev H. Kruger, Merlind Mucke, Monika Reuter   +2 more
core  

Rational Design and Development of Organic Afterglow Nanoparticles for Image‐Guided Interventions

Small Methods, EarlyView.
This review outlines the rational design, synthesis, and standardized characterization of organic afterglow nanoparticles for biomedical imaging and therapy. Mechanistic insights into chemically initiated electron exchange luminescence and reactive oxygen species‐mediated processes are connected to molecular engineering and nanoparticle fabrication ...
Asma Harun, Isabella Vasquez, Hannah Bowman, Robert Posey, Zhongkui Hong, Joshua Tropp, Indrajit Srivastava   +6 more
wiley   +1 more source

Human SLX4 Is a Holliday Junction Resolvase Subunit that Binds Multiple DNA Repair/Recombination Endonucleases

, 2009
SummaryStructure-specific endonucleases resolve DNA secondary structures generated during DNA repair and recombination. The yeast 5′ flap endonuclease Slx1-Slx4 has received particular attention with the finding that Slx4 has Slx1-independent key ...
Yates, John R., McGowan, Clare, Ruse, Cristian, Scaglione, Sarah, McGowan, Clare H., Yates, John, Taylor, Ewan R., Fuchs, Robert P., Gaillard, Pierre-Henri L., Fuchs, Robert,, Fekairi, Samira, Chahwan, Charly, Tissier, Agnès, Coulon, Stéphane, Dong, Meng-Qiu, gaillard, pierre-henri,, Russell, Paul, Taylor, Ewan   +17 more
core   +1 more source

Untreated Hair Dye Effluents Enter the Environment: Are They a Threat to Human Health?

Environmental Toxicology, EarlyView.
ABSTRACT The effluents generated during the process of hair dyeing exhibit a complex composition, comprising chemical compounds with varying toxicity levels. While the adverse impact of hair dyes on human health is acknowledged, there is a notable absence of studies addressing the toxicity associated with effluents produced during these activities. The
Letícia Cristina Gonçalves, Matheus Mantuanelli Roberto, Adriana Fabiana Corrêa da Silva, Maria Aparecida Marin‐Morales   +3 more
wiley   +1 more source

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes

, 2017
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for ...
Dryden, David T. F., Blumenthal, Robert M., Morgan, Richard D., Hornby, David P., Rao, Desirazu N., Janulaitis, Arvydas, Reich, Norbert, Wilson, Geoffrey G., Trautner, Thomas A., Halford, Stephen E., Josephsen, Jytte, Selker, Eric U., Vitor, Jorge M. B., Gromova, Elizaveta S., Piekarowicz, Andrzej, Hattman, Stanley, Marinus, Martin G., Roberts, Richard J., Heitman, Joseph, Bickle, Thomas A., Kiss, Antal, Pingoud, Alfred, Degtyarev, Sergey Kh, Kong, Huimin, Gumport, Richard I., Klaenhammer, Todd R., Lacks, Sanford, Xu, Shuang‐yong, Bhagwat, Ashok S., Krüger, Detlev H., Murray, Noreen E., Shaw, Pang‐Chui, Bestor, Timothy, Stein, Daniel C., Jeltsch, Albert, Kobayashi, Ichizo, Stoddard, Barry L., Bitinaite, Jurate, Szybalski, Waclaw, Dybvig, Kevin, Miyahara, Michiko, Belfort, Marlene, Raleigh, Elisabeth, Van Etten, James L., Firman, Keith, Nagaraja, Valakunja, Repin, Vladimir E.   +46 more
core  

Iflaviruses in arthropods: when small is mighty

Insect Science, EarlyView.
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia, Hannah‐Isadora Huditz, Monique M. van Oers, Vera I.D. Ros   +3 more
wiley   +1 more source

Iron Physiology and Its Impact on Atopic Diseases: An EAACI Taskforce Report

Allergy, EarlyView.
ABSTRACT Iron is essential for oxygen transport, energy metabolism, and immune regulation. Yet iron deficiency is the most common micronutrient disorder across all age groups, affecting nearly one quarter of the global population. Iron deficiency triggers nutritional immunity, a host defense mechanism that withholds and redistributes iron, contributing
Franziska Roth‐Walter, Ioana Agache, Beatriz Cabanillas, Roberto Berni Canani, Pasquale Comberiati, Holger Garn, Cristina Gomez‐Casado, Karin Hufnagl, Ekaterina Khaleva, Gregorio P. Milani, Daniel Munblit, Nikolaos Douladiris, Liam O'Mahony, Frank Redegeld, Carmen Riggioni, Peter K. Smith, Betty van Esch, Emilia Vassilopoulou, Carina Venter, Diego G. Peroni   +19 more
wiley   +1 more source