Results 131 to 140 of about 64,056 (293)

Cleavage of non-tRNA substrates by eukaryal tRNA splicing endonucleases

, 2001
Eukaryal tRNA splicing endonucleases use the mature domains of pre-tRNAs as their primary recognition elements. However, they can also cleave in a mode that is independent of the mature domain, when substrates are able to ...
TocchiniValentini GP, Fruscoloni P, Baldi MI   +2 more
core  

Sodium Ions Affect GPR17 Conformational States and Functionality

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Sodium is an allosteric modulator of several class‐A GPCR regulating active versus inactive conformation state and may be a critical issue for the optimization of crystallization procedures. Herein, the role of Na+ in the regulation of GPR17 conformational states was studied through MD simulations of GPR17 wild‐type and mutated on two ...
Luca Palazzolo, Simona Daniele, Davide Bianchi, Lara Russo, Stefano Capaldi, Maria Letizia Trincavelli, Ivano Eberini   +6 more
wiley   +1 more source

Molecular Basis for Uracil‐DNA‐Glycosylase to Identify DNA Damage in the Nucleosome

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The DNA base‐excision repair (BER) pathway is initiated by a glycosylase, such as uracil‐DNA‐glycosylase (UDG), which identifies and removes a wrong base incorporated in the DNA sequence. The very early steps in the identification of the DNA damage are crucial to the correct initiation of the repair chain, and become even more complex when ...
Safwen Ghediri, Ralf Blossey, Fabrizio Cleri   +2 more
wiley   +1 more source

SURVEY AND SUMMARY Diversity of Type II restriction endonucleases that require two DNA recognition sites

, 2003
Orthodox Type IIP restriction endonucleases, which are commonly used in molecular biological work, recognize a single palindromic DNA recognition sequence and cleave within or near this sequence.
Detlev H. Kruger, Merlind Mucke, Monika Reuter   +2 more
core  

RNA degradomics and proteomics reveal the mechanism of dsProsβ1‐mediated proteasome targeting in Psylliodes chrysocephala

Pest Management Science, EarlyView.
RNA degradomics revealed dsProsβ1‐derived siRNA‐mediated mRNA cleavage events, mainly at uracil‐guanine and adenine‐adenine pairs. Proteasome inhibition via dsProsβ1 increased mitochondrial and cytoskeletal proteins while reducing translation‐related and mRNA‐binding proteins.
Doga Cedden, Gözde Güney, Michael Rostás, Stefan Scholten   +3 more
wiley   +1 more source

Target‐site mutations and phenotypic failure in Myzus persicae against pyrethroids and carbamates in central Europe

Pest Management Science, EarlyView.
Molecular screening of 140 field‐collected Myzus persicae aphids revealed target‐site mutations L1014F, M918L, M918T, and L932F in the sodium channel and S431F in Ace2. The M918L mutation was the primary driver of high pyrethroid (cypermethrin) resistance, while S431F served as a definitive marker for carbamate (pirimicarb) resistance.
Jitka Stara, Jan Hubert, Pavla Pabiskova, Frantisek Kocourek   +3 more
wiley   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Nature‐Inspired Histidine and Guanidine Modification of Magnetic Nanoparticles for Urinary DNA Liquid Biopsy

Small Methods, EarlyView.
Nature‐inspired bi‐functionalized magnetic nanoparticles are engineered for the rapid, non‐invasive extraction of cell‐free DNA from unprocessed urine for liquid biopsy. These cationic particles leverage electrostatic interactions to outperform conventional kits, enhancing the recovery of short DNA fragments while maintaining DNA integrity.
Dina Haddad, Ishtiaq Ahmed, Manthos Pitoulias, Richard Mair, Ljiljana Fruk   +4 more
wiley   +1 more source

A dual gating mechanism controls target-strand cleavage in Cas12j: Implications for engineering efficient nickases. [PDF]

Protein Sci
Abstract The rapid expansion of CRISPR technologies has unveiled a diverse repertoire of RNA‐guided endonucleases, among them the compact Cas12j, which has emerged as a promising genome‐editing tool. Cas12j cleaves the two DNA strands sequentially; however, the molecular mechanism that regulates this cleavage remains incompletely understood.
Karvounis IG, Daskalakis V.
europepmc   +2 more sources

Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi

Yeast, EarlyView.
Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng, Masahiro Tominaga, Chengqiang Wang, Robert E. Speight, Jun Ishii   +4 more
wiley   +1 more source