Results 151 to 160 of about 60,825 (304)

Recurrent Modular Assembly Drives Independent Origins of Group II Introns Encoding LAGLIDADG Homing Endonucleases. [PDF]

Genome Biol Evol
Liu F, Jin S, Du Q.
europepmc   +1 more source

Hairpin probes for real-time assay of restriction endonucleases

, 2002
New fluorogenic probes for restriction endonucleases based on molecular beacons were developed. These hairpin probes were single-stranded oligonucleotides that had stem-and-loop structure and carried 5'-fluorophor moiety and 3'-quencher moiety.
Liang, JX, 张永有, 李庆阁, Zhang, YY, Zhu, YB, Li, QG   +5 more
core  

Gene Editing for Haemophilia—The Next Frontier

Haemophilia, EarlyView.
ABSTRACT The recently approved haemophilia A and B gene therapies via adeno‐associated virus (AAV) showed a promising therapeutic response after a single injection, but there are still limitations, including the potential loss of transgene expression and restriction in adults.
Mirko Pinotti, Gregory A. Newby, Sundar Selvaraj, Steven W. Pipe   +3 more
wiley   +1 more source

Toxic Mechanism of Norfloxacin on <i>Chlamydomonas reinhardtii</i> by Triggering Programmed Cell Death. [PDF]

Plants (Basel)
Du X, Huang L, Lai M, Xu H, Huang T, Hu R, Ma J, Wei Y, Zuo Z.   +8 more
europepmc   +1 more source

Intrinsic Cleavage Inhibition by Thermophilic Type II-S Restriction Endonucleases

, 2019
Restriction endonucleases (REs) are the cornerstone research tools used in molecular biology and biotechnology. These enzymes are essential to several ground-breaking achievements, including DNA cloning, gene editing, and genome mapping.
Ciccone, Dino Jake
core  

Emerging experimental and bioinformatic approaches in RNA interference‐based pest control research

Insect Molecular Biology, EarlyView.
Double‐stranded RNA (dsRNA) visualization, RNA‐induced silencing complex (RISC)‐bound sRNA‐seq and RNA degradomics allow investigating dsRNA uptake and processing, guide small interfering RNA selection and RISC‐mediated target mRNA cleavages. Transcriptomic and proteomic profiles of genome‐wide responses to dsRNA, including off‐target and compensatory ...
Doga Cedden
wiley   +1 more source

Nucleotide excision repair and poly(ADP-ribose) polymerase 1 cooperate in HBV cccDNA synthesis: A potential viral therapeutic target. [PDF]

JHEP Rep
Hung HC, Tsai HW, Li YT, Liu WC, Cheng KH, Yen CJ, Lee YP, Fang YH, Chuang CH, Wu HL, Chang TT, Huang W.   +11 more
europepmc   +1 more source

Transcriptomic and proteomic signatures underlying nymphal adaptation and foam production in the forage pest Mahanarva spectabilis

Insect Molecular Biology, EarlyView.
RNA‐seq and Batelli gland proteomics of fifth‐instar Mahanarva spectabilis nymphs reveal transcripts and proteins associated with xylem feeding, foam production and environmental interaction. Functional annotation identified genes involved in osmoregulation, detoxification, chemosensation and stress responses, while proteomic analysis confirmed ...
Monique da Silva Bonjour, Angelo José Rinaldi, Eulálio Gutemberg Dias dos Santos, Gabriely Teixeira Bhering Faria, Ana Márcia Escocard de Azevedo Manhães, Jorge Fernando Pereira, Alexander Machado Auad, Maria Goreti de Almeida Oliveira, Humberto Josué de Oliveira Ramos   +8 more
wiley   +1 more source

Diversity and functional characterization of HNH endonucleases encoded by lactococcal <i>Skunavirus</i> phages. [PDF]

Microb Genom
Yu JH, Cambillau C, Lugli GA, Ventura M, Nauta A, Mahony J, van Sinderen D.   +6 more
europepmc   +1 more source

Cytosolic delivery of bacterial metabolites by riboflavin transporters promotes MR1 antigen presentation and MAIT cell recognition

Immunology &Cell Biology, EarlyView.
VitBAg antigen presentation is promoted through a cytosolic pathway that reaches ligand‐receptive MR1 to present to MAIT cells, like the riboflavin transport pathway. Riboflavin solute carrier transporters can promote this MR1 presentation, but they are not essential for this role, suggesting redundant pathways to uptake VitBAg.
Sebastian Cruz‐Gomez, Hui Jing Lim, Brunda Nijagal, Yuting Yang, Nicole Lau, Jeffrey YW Mak, David P Fairlie, Hayley Newton, Mariolina Salio, Jose A Villadangos, Hamish EG McWilliam   +10 more
wiley   +1 more source