Targeting cccDNA for Degradation: Endogenous- and Exogenous-Based Methods of Nuclear-Localized DNA Destruction in the Context of Chronic Hepatitis B Infection. [PDF]
VirusesThompson C, Meier-Stephenson V.
europepmc +1 more source
Arsenite methyltransferase 3 is required for mitochondrial function and hepatic lipid metabolism
The FEBS Journal, EarlyView.AS3MT is a protein that helps the body process arsenic, a naturally occurring toxin found in water and soil. We discovered it also helps cells make and use energy properly. Without AS3MT, cells' mitochondria do not work well, leading to fatty liver and reduced activity. Increasing AS3MT in liver cells restores energy and protects against arsenic damage,
Patrice Delaney +10 more
wiley +1 more source
Revisiting hydrolytic mechanisms of bacterial RNase HI: Towards clarification of single mobile Mg<sup>2+</sup> action in nuclease reactions. [PDF]
Biophys PhysicobiolMorikawa K, Oyama T, Oda M.
europepmc +1 more source
Hairpin probes for real-time assay of restriction endonucleases
, 2002 New fluorogenic probes for restriction endonucleases based on molecular beacons were developed. These hairpin probes were single-stranded oligonucleotides that had stem-and-loop structure and carried 5'-fluorophor moiety and 3'-quencher moiety.
Liang, JX +5 more
core
The FEBS Journal, EarlyView.Chromosomal DNA double‐strand breaks (DSBs) are repaired by homologous recombination and nonhomologous end joining (NHEJ). Recent work has additionally established theta‐mediated end‐joining (TMEJ) as a mechanism for DSB joining. Cells lacking NHEJ and TMEJ can repair DSBs in a homology‐dependent manner.
Shinta Saito +6 more
wiley +1 more source
Unlocking the power and potential of the Cas12 family members and their ancestors for crop genome editing. [PDF]
J Integr Plant BiolXia L, Lai J, Zhu JK.
europepmc +1 more source
TOP1MT rs2293925 is an enhancer‐active regulatory SNP that shapes mitochondrial R‐loop dynamics
The FEBS Journal, EarlyView.This study shows how a common genetic variant of mitochondrial topoisomerase 1 (TOP1MT rs2293925) can influence mitochondrial gene regulation, DNA topology, and formation of noncanonical nucleic acid structures such as R‐loops. By linking this enhancer‐active variant to mitochondrial nucleic acid stress in cellular contexts relevant to amyotrophic ...
Dóra Varga +15 more
wiley +1 more source
Tankyrase‐2 regulates adipocyte differentiation through AMPK/mTOR signaling
The FEBS Journal, EarlyView.Tankyrase 2 (TNKS2), a PARP family member, underpins adipocyte differentiation. TNKS2 controls LKB1 protein level, and consequently, the activity of the mTOR/AMPK system and adipocyte differentiation through a layered process. Pharmacological inhibition of TNKS2 leaves other PARPs and DNA repair active and therefore represents a novel target in ...
Boglarka Rauch +7 more
wiley +1 more source
The activity, divergence, and evolutionary degradation of modern-day homing endonucleases and behavior of their reconstructed ancestors. [PDF]
Nucleic Acids ResYoung JC +6 more
europepmc +1 more source
The FEBS Journal, EarlyView.A tandem fluorescent mCherry–EGFP–LC3 reporter enables live‐cell visualisation of autophagy–lysosome dynamics in naked mole‐rat skin fibroblasts. Chloroquine‐induced lysosomal stress promotes accumulation of neutral LC3‐positive compartments and triggers pronounced LC3‐associated vacuolation.
Fangze Tong +6 more
wiley +1 more source