Functions and applications of enzymes in nucleic acid nanotechnology. [PDF]
Rong Q +7 more
europepmc +1 more source
Maize regeneration via somatic embryogenesis by ectopic expression of morphogenic factors TaGRF4‐TaGIF1 and ZmBBM. Summary Transformation is an indispensable tool for plant genetics and functional genomics. Although stable transformation in maize is no longer a major obstacle, there remains a need for accessible and efficient methods for academic ...
Zongliang Chen +8 more
wiley +1 more source
Nitro Reduction‐Based RNA Control and Ultrafast Release
A chemical RNA caging–uncaging strategy based on nitro reduction is described. Nitroaryl groups introduced via acylation temporarily block the functions of diverse RNAs in vitro and in living cells. Treatment with a diboronic acid–bipyridine mixture rapidly reduces the nitro groups, releasing RNAs and restoring their activity.
Yiran Zhao +6 more
wiley +2 more sources
Comprehensive and comparative genomic analysis of a novel strain, Peredibacter starrii HCB2-198, to identify features of the genus. [PDF]
Tan S +4 more
europepmc +1 more source
Circadian Clock Gates Verticillium dahliae Defence in Cotton by Modulating JAZs Expression
ABSTRACT The circadian clock plays critical roles in orchestrating temporal regulation of diverse physiological processes. However, its role in mediating root‐associated immunity against soil‐borne pathogens in crops remains poorly understood. Here, we show that cotton circadian clock gates nighttime resistance to vascular fungal pathogen Verticillium ...
Ping Wang +17 more
wiley +1 more source
Nutrient disturbance in a shallow aquaculture pond impacts Microcystis gene expression but does not impact bacterial community function during bloom conditions. [PDF]
Gladfelter MF +3 more
europepmc +1 more source
RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee +3 more
wiley +1 more source
Modular determinants of cleavage preference in GIY-YIG nucleases revealed by block-based DNA shuffling and directed evolution. [PDF]
Loedige KW +4 more
europepmc +1 more source
Comprehensive genome analysis uncovers the diversity of jumbo phages in the pig gut microbiome. [PDF]
Wei C, Chen Z.
europepmc +1 more source
SIRT1, an NAD+‐dependent deacetylase, supports cognitive resilience by coordinating neuroprotection, synaptic plasticity, autophagy–lysosomal proteostasis, and mitochondrial energy homeostasis. Reduced or dysregulated SIRT1 is associated with protein aggregation, neuroinflammation, mitochondrial dysfunction, synaptic loss, and cognitive decline in ...
Jiabin Duan +6 more
wiley +1 more source

