Results 231 to 240 of about 60,898 (304)

Asymmetrical recognition and processing of double-strand breaks formed during DNA replication. [PDF]

Proc Natl Acad Sci U S A
Johnson MJ, Kimble MT, Jeong S, Sane A, Symington LS.   +4 more
europepmc   +1 more source

Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila

Advanced Science, Volume 13, Issue 31, 4 June 2026.
Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu, Xuedi Zhang, Ying Zhou, Jie Zheng, Angyang Sun, Kailong Zhuang, Weiwei Bao, Guanjun Gao   +7 more
wiley   +1 more source

Phage-encoded homing endonucleases attenuate bacterial immunity

Mahata T, Kanarek K, Ragavan RM, Haldar A, Shur G, Yehia R, Burstein D, Haitin Y, Qimron U, Salomon D.   +9 more
europepmc   +1 more source

Development and optimization of host DNA depletion in blood cultures using a saponin and salt-activated nuclease-based method. [PDF]

Front Microbiol
Ali J, Bellankimath AB, Hira J, Chapagain C, Opgård ST, Simonsen GS, Ahmad R.   +6 more
europepmc   +1 more source

EEPD1 Inhibition Unleashes Antitumor Immunity in Colorectal Cancer by Activating the cGAS‐STING Pathway

Advanced Science, Volume 13, Issue 34, 19 June 2026.
In colorectal cancer, high expression of the DNA repair protein EEPD1 correlates with immune exclusion and poor prognosis. This study demonstrates that EEPD1 depletion induces genomic instability, leading to cytosolic DNA accumulation and subsequent activation of the cGAS‐STING‐type I interferon pathway. This cascade remodels the tumor microenvironment
Liyun Huo, Chong Wu, Xiaobo Li, Caina Ma, Jiamin Huang, Zishuo Chen, Weiling He, Tianyu Tao, Weijing Zhang   +8 more
wiley   +1 more source

The Biology of Nucleus-Forming Jumbo Phages. [PDF]

Annu Rev Genet
Birkholz EA, Armbruster EG, Pogliano J.
europepmc   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, Volume 13, Issue 32, 9 June 2026.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Accurate, comprehensive database of group I introns and their homing endonucleases. [PDF]

Bioinform Adv
Sellés Vidal L, Noma T, Yokobayashi Y.
europepmc   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, Volume 13, Issue 32, 9 June 2026.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

Emerging therapeutic strategies for mitochondrial DNA-related diseases. [PDF]

Cell Rep Med
Shi R, Falabella M, Aref J, Hanna MG, Minczuk M, Viscomi C, Pitceathly RDS.   +6 more
europepmc   +1 more source