Results 241 to 250 of about 64,056 (293)
Some of the next articles are maybe not open access.
A mammalian nicking endonuclease
Biochemistry, 1976Purification and properties are described for an endonuclease isolated from calf thymus which attacks double-stranded, unmodified DNA, primarily by making single-strand breaks. No detectable acid-soluble products arise from the reaction. Double-strand breaks may occasionally be produced by the introduction of single-strand breaks on opposite strands in
Lavin, Martin F. +5 more
openaire +4 more sources
Current Protocols in Molecular Biology, 2011
AbstractReaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also ...
openaire +2 more sources
AbstractReaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also ...
openaire +2 more sources
Characterization of homing endonucleases
Methods, 2002Homing endonucleases are a class of site-specific DNA endonucleases encoded by open reading frames within introns and inteins. They initiate the mobility of their host element by recognizing intronless or inteinless alleles of their host gene and making a double-strand break.
Joseph C, Kowalski, Victoria, Derbyshire
openaire +2 more sources
Characterization of mRNA Endonucleases
Methods, 1999Endonucleases are key effectors of mRNA degradation, particularly for mRNAs whose turnover rates are regulated by extracellular stimuli. The rapid clearance of mRNA degradation products in vivo and the need to selectively identify mRNA endonucleases in the presence of many other cellular ribonucleases make the study of these enzymes particularly ...
D R, Schoenberg, K S, Cunningham
openaire +2 more sources
The Journal of Biochemistry, 1978
It was confirmed by polyacrylamide gel electrophoresis that isolated 16S rRNA was cleaved by the active component (protein A) or the active fragment (T2A) of colicin E3. However, the degradation was random, in contrast with the specific cleavage observed in the interaction of colicin E3 with ribosomes.
S, Ohno, K, Imahori
openaire +2 more sources
It was confirmed by polyacrylamide gel electrophoresis that isolated 16S rRNA was cleaved by the active component (protein A) or the active fragment (T2A) of colicin E3. However, the degradation was random, in contrast with the specific cleavage observed in the interaction of colicin E3 with ribosomes.
S, Ohno, K, Imahori
openaire +2 more sources
Biochimica et biophysica acta-Molecular basis of disease, 2014
Mouse transgenesis has been instrumental in determining the function of genes in the pathophysiology of human diseases and modification of genes by homologous recombination in mouse embryonic stem cells remains a widely used technology. However, this approach harbors a number of disadvantages, as it is time-consuming and quite laborious.
Wijshake, Tobias +2 more
openaire +1 more source
Mouse transgenesis has been instrumental in determining the function of genes in the pathophysiology of human diseases and modification of genes by homologous recombination in mouse embryonic stem cells remains a widely used technology. However, this approach harbors a number of disadvantages, as it is time-consuming and quite laborious.
Wijshake, Tobias +2 more
openaire +1 more source
Endonucleases and apoptosis in animals
Biochemistry (Moscow), 2012Endonucleases are the main instruments of obligatory DNA degradation in apoptosis. Many endonucleases have marked processive action; initially they split DNA in chromatin into very large domains, and then they perform in it internucleosomal fragmentation of DNA followed by its hydrolysis to small fragments (oligonucleotides).
N I, Aleksandrushkina, B F, Vanyushin
openaire +2 more sources
Homing in on an endosymbiotic endonuclease
Current Biology, 1992There was a time when life as a molecular biologist was relatively simple: genes were genes easily recognizable by a beginning, an end and a coding sequence in between. The arrival of introns complicated things for a while: one had to get used to the idea that some genes were in pieces, perhaps always had been.
openaire +2 more sources
Restriction endonucleases in Azospirillum
Gene, 1985Azospirillum brasilense, A. amazonense, and A. lipoferum strains were screened for restriction endonucleases using phage lambda DNA. The extract of A. brasilense 29711 cleaved lambda DNA into specific fragments. It was concluded that this strain possesses a class II restriction endonuclease which was named AbrI.
G, Schwabe +2 more
openaire +2 more sources
Virology, 1977
Abstract An endonuclease was found to associate with purified T4 particles. Its activity was low with intact phages but became distinct when phage particles were disrupted by osmotic shock, and more distinct when they were disassembled by guanidine.
openaire +2 more sources
Abstract An endonuclease was found to associate with purified T4 particles. Its activity was low with intact phages but became distinct when phage particles were disrupted by osmotic shock, and more distinct when they were disassembled by guanidine.
openaire +2 more sources