Results 261 to 270 of about 65,125 (289)
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2000
Programmed cell death (PCD) involves hydrolysis of genomic DNA, which must be catalyzed by endonuclease(s) capable of digesting dsDNA. Plants have two major classes of endonucleases active towards dsDNA, Zn2+-dependent endonuclease and Ca2+-dependent endonuclease.
M, Sugiyama +3 more
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Programmed cell death (PCD) involves hydrolysis of genomic DNA, which must be catalyzed by endonuclease(s) capable of digesting dsDNA. Plants have two major classes of endonucleases active towards dsDNA, Zn2+-dependent endonuclease and Ca2+-dependent endonuclease.
M, Sugiyama +3 more
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Characterization of homing endonucleases
Methods, 2002Homing endonucleases are a class of site-specific DNA endonucleases encoded by open reading frames within introns and inteins. They initiate the mobility of their host element by recognizing intronless or inteinless alleles of their host gene and making a double-strand break.
Victoria Derbyshire, Joseph C. Kowalski
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Current Protocols in Molecular Biology, 2011
AbstractReaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also ...
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AbstractReaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also ...
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The Journal of Biochemistry, 1978
It was confirmed by polyacrylamide gel electrophoresis that isolated 16S rRNA was cleaved by the active component (protein A) or the active fragment (T2A) of colicin E3. However, the degradation was random, in contrast with the specific cleavage observed in the interaction of colicin E3 with ribosomes.
Kazutomo Imahori, Shigeo Ohno
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It was confirmed by polyacrylamide gel electrophoresis that isolated 16S rRNA was cleaved by the active component (protein A) or the active fragment (T2A) of colicin E3. However, the degradation was random, in contrast with the specific cleavage observed in the interaction of colicin E3 with ribosomes.
Kazutomo Imahori, Shigeo Ohno
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Angewandte Chemie International Edition in English, 1978
AbstractRestriction endonucleases are enzymes commonly found in bacteria which can fragment DNA. Cleavage of the DNA molecule takes place after recognition of a nucleotide sequence which is specific for each restriction endonuclease. Some of the enzymes split the DNA within the recognition sequence. This property allows these enzymes to be used for the
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AbstractRestriction endonucleases are enzymes commonly found in bacteria which can fragment DNA. Cleavage of the DNA molecule takes place after recognition of a nucleotide sequence which is specific for each restriction endonuclease. Some of the enzymes split the DNA within the recognition sequence. This property allows these enzymes to be used for the
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An Endonuclease Responsible for Apoptosis
1996Apoptosis is a form of cell death that plays important roles in physiological and pathological phenomena as diverse as embryogenesis, metamorphosis, hemopoiesis, and carcinogenesis (Kerr et al. 1972; Wyllie 1980; Berger 1985; Carson et al. 1986; Ucker 1987; Smith et al. 1989; Dive and Hickman 1991; Groux et al. 1992; Cohen et al.
Daisuke Shiokawa, Sei-ichi Tanuma
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Endonucleases and apoptosis in animals
Biochemistry (Moscow), 2012Endonucleases are the main instruments of obligatory DNA degradation in apoptosis. Many endonucleases have marked processive action; initially they split DNA in chromatin into very large domains, and then they perform in it internucleosomal fragmentation of DNA followed by its hydrolysis to small fragments (oligonucleotides).
N. I. Aleksandrushkina +1 more
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Characterization of mRNA Endonucleases
Methods, 1999Endonucleases are key effectors of mRNA degradation, particularly for mRNAs whose turnover rates are regulated by extracellular stimuli. The rapid clearance of mRNA degradation products in vivo and the need to selectively identify mRNA endonucleases in the presence of many other cellular ribonucleases make the study of these enzymes particularly ...
Daniel R. Schoenberg +1 more
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Digestion of DNA with Restriction Endonucleases [PDF]
Current Protocols in Neuroscience, 1992 AbstractRestriction endonucleases recognize short DNA sequences and cleave double‐stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology.
K D, Bloch, B, Grossmann
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Restriction endonucleases in Azospirillum
Gene, 1985Azospirillum brasilense, A. amazonense, and A. lipoferum strains were screened for restriction endonucleases using phage lambda DNA. The extract of A. brasilense 29711 cleaved lambda DNA into specific fragments. It was concluded that this strain possesses a class II restriction endonuclease which was named AbrI.
Gerhard Schwabe +2 more
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