Results 271 to 280 of about 65,125 (289)

Homing in on an endosymbiotic endonuclease

Current Biology, 1992
There was a time when life as a molecular biologist was relatively simple: genes were genes easily recognizable by a beginning, an end and a coding sequence in between. The arrival of introns complicated things for a while: one had to get used to the idea that some genes were in pieces, perhaps always had been.
openaire   +3 more sources

Endonuclease from Proteus mirabilis

Applied Biochemistry and Microbiology, 2001
Two isoforms of nuclease displaying DNase and RNase activities were found in the culture liquid and periplasm of Proteus mirabilis. The enzyme was isolated from the periplasm and then purified to a functionally homogeneous state. The nuclease was equally potent in cleaving denatured and native DNAs by the endonuclease mechanism and was designated Pm ...
Salikhova Z., Sokolova R., Ponomareva A., Yusupova D.   +3 more
openaire   +7 more sources

Characterization of the Escherichia coli x-ray endonuclease, endonuclease III

Biochemistry, 1983
The X-ray endonuclease endonuclease III of Escherichia coli has been purified to apparent homogeneity by using the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The most purified fraction shows endonucleolytic activity against apurinic and apyrimidinic (AP) sites and a dose-dependent response to DNA that has been X irradiated,
Susan S. Wallace, Harold L. Katcher
openaire   +3 more sources

Endonuclease of T4 ghosts

Virology, 1977
Abstract An endonuclease was found to associate with purified T4 particles. Its activity was low with intact phages but became distinct when phage particles were disrupted by osmotic shock, and more distinct when they were disassembled by guanidine.
openaire   +3 more sources

A homing endonuclease with a switch: Characterization of a twintron encoded homing endonuclease

Fungal Genetics and Biology, 2014
The small ribosomal subunit gene residing in the mitochondrial DNA of the thermophilic fungus Chaetomium thermophilum var. thermophilum La Touche DSM 1495 is interrupted by a twintron at position mS1247. The mS1247 twintron represents the first mixed twintron found in fungal mtDNA, composed of an external group I intron encoding a LAGLIDADG open ...
Georg Hausner, Tuhin Kumar Guha
openaire   +3 more sources

[2] Directory of restriction endonucleases

1979
Publisher Summary This chapter serves as a directory to the restriction endonucleases that have not been characterized. All endonucleases which cleave DNA at a specific sequence have been considered to be restriction enzymes, although in most cases there is no direct genetic evidence for the presence of a host-controlled restriction-modification ...
openaire   +4 more sources

Ultraviolet Damage Endonuclease (Uve1p):  A Structure and Strand-Specific DNA Endonuclease

Biochemistry, 2000
Schizosaccharomyces pombe ultraviolet damage endonuclease (UVDE or Uve1p) performs the initial step in an alternative excision repair pathway for UV-induced DNA damage. This DNA repair pathway was originally thought to be specific for UV damage. However, the broad substrate specificity of Uve1p suggests a more general role for this enzyme.
Paul W. Doetsch, Balveen Kaur
openaire   +3 more sources

An Endonuclease Tango

Science, 2008
BIOCHEMISTRY The degradation of mRNA transcripts is an important cellular mechanism contributing to the regulation of gene expression. In Escherichia coli , the first step of this process involves internal cleavage of mRNA into fragments by the endoribonuclease RNase E.
openaire   +2 more sources

An endonuclease at last

Nature, 1998
One of the main clues that apoptosis is a ‘programmed’ cell death is the production of ‘chromatin ladders’ — digestion of DNA into regularly sized fragments. The nuclease responsible for this cleavage has now been identified. Found as an inactive form within the cytoplasm, this nuclease is activated by caspase-3 digestion, earning it the name caspase ...
openaire   +2 more sources

The Type IIB restriction endonucleases

Biochemical Society Transactions, 2010
The endonucleases from the Type IIB restriction–modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder ...
Marshall, JJT, Halford, SE
openaire   +4 more sources