Results 131 to 140 of about 5,190 (143)
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Purification of cytosolic, latent endoribonuclease from porcine thyroid
Archives of Biochemistry and Biophysics, 1982Abstract An alkaline endoribonuclease was purified 1800-fold from the cytosolic, latent ribonuclease fraction of porcine thyroids by gentle procedures specifically designed to exclude both heating and acidification steps. Polyacrylamide gel electrophoresis revealed a broad peak of enzyme activity that was coincident with the stained protein band.
E E, Button, R, Guggenheimer, F J, Kull
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The Cmr complex: an RNA-guided endoribonuclease
Biochemical Society Transactions, 2013The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) system protects prokaryotes from infection by viruses and other potential genome invaders. This system represents an inheritable and adaptable immune system that is mediated by large ribonucleoprotein complexes, the CRISPR–Cas effector complexes.
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Characterization of an Endoribonuclease in Rat-Liver Nucleoli
European Journal of Biochemistry, 1974Synthetic RNAs, i.e. [3H]poly(U) and [14C]poly(A), and natural RNAs, i.e. 16-S and 23-S Escherichia coli ribosomal [14C]RNA and MS2 coliphage [3H]RNA, were incompletely hydrolyzed by a rat liver nucleolar enzyme as measured by solubilization of radioactivity following ethanol precipitation.
A, Boctor, A, Grossman, W, Szer
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Cleavage site recognition by the tRNA splicing endoribonuclease
Gene, 1993A single tRNA-splicing endoribonuclease can cleave several precursors. In addition to the conserved nucleotides (nt), there are positions in the mature domain that, though not always occupied by the same nt, nevertheless play a fundamental role in intron excision reaction. The elements of the recognition set (invariant nt, nt at the cardinal positions)
G P, Tocchini-Valentini +3 more
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[Synthetic endoribonucleases].
Molekuliarnaia biologiia, 1993The data on the synthesis of RNA-fragments with endoribonuclease activity were reviewed. In the framework of the hammerhead model refined by Haselof and Gerlach the contribution of certain nucleotides or functional groups in ribozyme catalytic activity and the role of double helices are considered in detail.
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Endoribonuclease IV. 2. Further Investigation on the Specificity
European Journal of Biochemistry, 1976The poly(A)-specific endoribonuclease IV produces oligo(A) fragments of a chain length of 10 AMP nucleotides. One enzyme molecule performs 1700 cleavages per min; the cleavages occur randomly. The endoribonuclease IV is a nuclear enzyme which is present in the oviduct of quails in a concentration of 40 000 enzyme molecules per cell. Poly(A) segments on
W E, Müller +3 more
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Staphylococcus aureus endoribonuclease III purification and properties.
Methods in enzymology, 2008Staphylococcus aureus ribonuclease III (Sa-RNase III) belongs to the enzyme family known to process double-stranded RNAs consisting of two turns of the RNA helix. Although the enzyme is thought to play a role in ribosomal RNA processing and gene regulation, the deletion of the rnc gene in S. aureus does not affect cell growth in rich medium. S.
Chevalier, C. +6 more
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Recombinant proteasome alpha-type subunits exhibit endoribonuclease activity
Cell and Tissue Biology, 201126S proteasome is a multi-subunit protein complex that consists of the regulatory 19S and the catalytic 20S subcomplexes. The major cellular function of the proteasome is protein degradation. It has been found recently that the 20S particle, besides its proteolytic activity, also possesses endoribonuclease activity.
O A, Fedorova +3 more
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RNA Recognition and Cleavage by Sequence-Specific Endoribonucleases
Protein & Peptide Letters, 2007Inactivation of RNA molecules by sequence-specific endoribonucleolytic cleavage is a subtle mechanism by which cells regulate gene expression. Sequence-specific endoribonucleases can recognize and cleave particular phosphodiester bonds confined within hundreds/thousands of chemically similar bonds.
Fakhri, Saïda, Benoît, Odaert
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