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Bioengineering and stem cells for corneal endothelial cell therapy
Acta Ophthalmologica, 2013AbstractThe global planetary scarcity for corneal donation pushes research teams to develop substitution strategies. The worldwide success of endothelial keratoplasty (EK) has profoundly simplified the paradigm of corneal bioengineering since the technical and biological requirements for EK is far simpler than for full thickness corneas.
Fabien Forest +13 more
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Endothelial progenitor cells from embryonic stem cells
Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology, 2003Stem cell research has gained significant interest due to their potential to repopulate damaged or diseased cell tissues. Pluripotent embryonic stem (ES) cells, able to rapidly and stably proliferate in vitro, provide an excellent system for studying cellular differentiation events. Our lab is particularly interested in stem cell differentiation of the
Robert M. Nerem +4 more
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Growth and endothelial differentiation of adipose stem cells on polycaprolactone
Journal of Biomedical Materials Research Part A, 2011AbstractAdipose tissue is a readily available source of multipotent adult stem cells for use in tissue engineering/regenerative medicine. Various growth factors have been used to stimulate acquisition of endothelial characteristics by adipose‐derived stem cells (ADSC). Herein, we study the growth and endothelial differentiation potential of ADSC seeded
Marino Gerardo +7 more
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Stem Cells and Development, 2017
Wnts are secreted glycoproteins that regulate stem cell self-renewal, differentiation, and cell-to-cell communication during embryonic development and in adult tissues.
J. McBride +5 more
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Wnts are secreted glycoproteins that regulate stem cell self-renewal, differentiation, and cell-to-cell communication during embryonic development and in adult tissues.
J. McBride +5 more
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Biological considerations of mesenchymal stem cells and endothelial progenitor cells
Injury, 2008Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) have been demonstrated as an attractive cell source for tissue engineering applications because of their ability to be isolated and expanded. Even though MSCs and EPCs constitute a powerful candidate cell type for regenerative medicine, more knowledge in terms of their biological ...
Argiris Papathanasopoulos +1 more
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Hypoxia-induced apoptosis in endothelial cells and embryonic stem cells
Apoptosis, 2005To evaluate the influence of hypoxia and molecular events in endothelial and embryonic stem cells.Human umbilical vein endothelial cells (HUVECs) and mouse embryoid body (EB) cells were subjected to hypoxic conditions for different time courses. DNA fragmentation assay, quantification of apoptotic cells by TUNEL assay measured by flowcytometry, and ...
Fon-Jou Hsieh +5 more
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Mesenchymal Stem Cells: The Role of Endothelial Cells and the Vasculature
2012Mesenchymal stem cells/multipotent stromal cells (MSCs) are a population of stem cells that have been isolated from virtually every adult tissue. MSCs are able to differentiate into skeletal lineages, such as bone, cartilage and fat, and as such represent valuable research tools for unravelling the complex mechanisms that control fate determination and
Paul G. Genever +3 more
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Endothelial and Hematopoietic Cell Fate of Human Embryonic Stem Cells
Trends in Cardiovascular Medicine, 2006The endothelial cells, lining the inside of blood vessels, and the blood-forming hematopoietic cells play crucial roles in vasculogenesis. The establishment of human embryonic stem cells (hESCs) provides a unique tool to study the early development of endothelial and hematopoietic cells, opening new avenues of research to explore organ vascularization ...
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Isolation of tissue-resident vascular endothelial stem cells from mouse liver
Nature Protocols, 2020Hisamichi Naito +8 more
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Haematopoietic stem cell induction by somite-derived endothelial cells controlled by meox1
Nature, 2014Phong D. Nguyen +14 more
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